Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins
Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) prese...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
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| Online Access: | https://hdl.handle.net/10356/105284 http://hdl.handle.net/10220/20681 |
| _version_ | 1826114998232940544 |
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| author | Tjin, Monica Suryana Chua, Alvin Wen Choong Ma, Dong Rui Lee, Seng Teik Fong, Eileen |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Tjin, Monica Suryana Chua, Alvin Wen Choong Ma, Dong Rui Lee, Seng Teik Fong, Eileen |
| author_sort | Tjin, Monica Suryana |
| collection | NTU |
| description | Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) present in the basement membrane in uninjured skin. To mimic these key integrin-ECM interactions, artificial ECM (aECM) proteins containing functional domains derived from laminin 5, type IV collagen, fibronectin, and elastin are prepared. Human skin keratinocyte cell responses on the aECM proteins are specific to the cell-binding domain present in each construct. Keratinocyte attachment to the aECM protein substrates is also mediated by specific integrin-material interactions. In addition, the aECM proteins are able to support the proliferation of keratinocyte stem cells, demonstrating their promise for use in skin tissue engineering. |
| first_indexed | 2024-10-01T03:48:21Z |
| format | Journal Article |
| id | ntu-10356/105284 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:48:21Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1052842020-06-01T10:13:43Z Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins Tjin, Monica Suryana Chua, Alvin Wen Choong Ma, Dong Rui Lee, Seng Teik Fong, Eileen School of Materials Science & Engineering DRNTU::Science::Biological sciences Cell-matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) present in the basement membrane in uninjured skin. To mimic these key integrin-ECM interactions, artificial ECM (aECM) proteins containing functional domains derived from laminin 5, type IV collagen, fibronectin, and elastin are prepared. Human skin keratinocyte cell responses on the aECM proteins are specific to the cell-binding domain present in each construct. Keratinocyte attachment to the aECM protein substrates is also mediated by specific integrin-material interactions. In addition, the aECM proteins are able to support the proliferation of keratinocyte stem cells, demonstrating their promise for use in skin tissue engineering. 2014-09-15T02:46:06Z 2019-12-06T21:48:46Z 2014-09-15T02:46:06Z 2019-12-06T21:48:46Z 2014 2014 Journal Article Tjin, M. S., Chua, A. W. C., Ma, D. R., Lee, S. T., & Fong, E. (2014). Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins. Macromolecular bioscience, 14(8), 1125-1134. 1616-5187 https://hdl.handle.net/10356/105284 http://hdl.handle.net/10220/20681 10.1002/mabi.201400015 en Macromolecular bioscience © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Science::Biological sciences Tjin, Monica Suryana Chua, Alvin Wen Choong Ma, Dong Rui Lee, Seng Teik Fong, Eileen Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins |
| title | Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins |
| title_full | Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins |
| title_fullStr | Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins |
| title_full_unstemmed | Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins |
| title_short | Human epidermal keratinocyte cell response on integrin-specific artificial extracellular matrix proteins |
| title_sort | human epidermal keratinocyte cell response on integrin specific artificial extracellular matrix proteins |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/105284 http://hdl.handle.net/10220/20681 |
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