Inorganic-organic hybrid nanoprobe for NIR-excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model
Hydrogen sulfide (H2S) is an important gaseous signaling agent mediated by many physiological processes and diseases. In order to explore its role in biological signaling, much effort has been focused on developing organic fluorescent probes to image H2S. However, these downconversion H2S probes are...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/105304 http://hdl.handle.net/10220/20511 |
| _version_ | 1826123547722907648 |
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| author | Zhou, Yi Chen, Wangqiao Zhu, Jixin Pei, Wenbo Wang, Chengyuan Huang, Ling Yao, Cheng Yan, Qinyu Huang, Wei Loo, Joachim Say Chye Zhang, Qichun |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Zhou, Yi Chen, Wangqiao Zhu, Jixin Pei, Wenbo Wang, Chengyuan Huang, Ling Yao, Cheng Yan, Qinyu Huang, Wei Loo, Joachim Say Chye Zhang, Qichun |
| author_sort | Zhou, Yi |
| collection | NTU |
| description | Hydrogen sulfide (H2S) is an important gaseous signaling agent mediated by many physiological processes and diseases. In order to explore its role in biological signaling, much effort has been focused on developing organic fluorescent probes to image H2S. However, these downconversion H2S probes are impractical for bio-imaging beyond a certain depth because of the short tissue penetration of UV/visible light (as an excitation source). In most circumstance, these probes are also not suitable for long-term assay due to photo-bleaching. Herein, a new design to detect H2S based on the coumarin-hemicyanine (CHC1)-modified upconversion nanophosphors is reported. This inorganic–organic integrated nanoprobe is demonstrated to display a fast response time with a large ratiometric upconversion luminescence (UCL) enhancement, and extraordinary photo-stability. CHC1-UCNPs not only can be used for ratiometric UCL monitoring of pseudo-enzymatic H2S production in living cells, but can also be used to identify the risk of endotoxic shock through ratiometric UCL imaging of tissue and measurement of endogenous H2S levels in plasma. The first ratiometric UCL H2S nanoprobe reported here may be further developed as the next-generation diagnostic tool for the detection of inflammatory-related diseases. |
| first_indexed | 2024-10-01T06:06:27Z |
| format | Journal Article |
| id | ntu-10356/105304 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:06:27Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1053042020-06-01T10:13:47Z Inorganic-organic hybrid nanoprobe for NIR-excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model Zhou, Yi Chen, Wangqiao Zhu, Jixin Pei, Wenbo Wang, Chengyuan Huang, Ling Yao, Cheng Yan, Qinyu Huang, Wei Loo, Joachim Say Chye Zhang, Qichun School of Chemical and Biomedical Engineering School of Materials Science & Engineering DRNTU::Engineering::Materials::Nanostructured materials Hydrogen sulfide (H2S) is an important gaseous signaling agent mediated by many physiological processes and diseases. In order to explore its role in biological signaling, much effort has been focused on developing organic fluorescent probes to image H2S. However, these downconversion H2S probes are impractical for bio-imaging beyond a certain depth because of the short tissue penetration of UV/visible light (as an excitation source). In most circumstance, these probes are also not suitable for long-term assay due to photo-bleaching. Herein, a new design to detect H2S based on the coumarin-hemicyanine (CHC1)-modified upconversion nanophosphors is reported. This inorganic–organic integrated nanoprobe is demonstrated to display a fast response time with a large ratiometric upconversion luminescence (UCL) enhancement, and extraordinary photo-stability. CHC1-UCNPs not only can be used for ratiometric UCL monitoring of pseudo-enzymatic H2S production in living cells, but can also be used to identify the risk of endotoxic shock through ratiometric UCL imaging of tissue and measurement of endogenous H2S levels in plasma. The first ratiometric UCL H2S nanoprobe reported here may be further developed as the next-generation diagnostic tool for the detection of inflammatory-related diseases. 2014-09-11T08:05:53Z 2019-12-06T21:48:58Z 2014-09-11T08:05:53Z 2019-12-06T21:48:58Z 2014 2014 Journal Article Zhou, Y., Chen, W., Zhu, J., Pei, W., Wang, C., Huang, L., et al. (2014). Inorganic-Organic Hybrid Nanoprobe for NIR-Excited Imaging of Hydrogen Sulfide in Cell Cultures and Inflammation in a Mouse Model. Small, in press. 1613-6810 https://hdl.handle.net/10356/105304 http://hdl.handle.net/10220/20511 10.1002/smll.201401867 en Small © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Engineering::Materials::Nanostructured materials Zhou, Yi Chen, Wangqiao Zhu, Jixin Pei, Wenbo Wang, Chengyuan Huang, Ling Yao, Cheng Yan, Qinyu Huang, Wei Loo, Joachim Say Chye Zhang, Qichun Inorganic-organic hybrid nanoprobe for NIR-excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model |
| title | Inorganic-organic hybrid nanoprobe for NIR-excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model |
| title_full | Inorganic-organic hybrid nanoprobe for NIR-excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model |
| title_fullStr | Inorganic-organic hybrid nanoprobe for NIR-excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model |
| title_full_unstemmed | Inorganic-organic hybrid nanoprobe for NIR-excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model |
| title_short | Inorganic-organic hybrid nanoprobe for NIR-excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model |
| title_sort | inorganic organic hybrid nanoprobe for nir excited imaging of hydrogen sulfide in cell cultures and inflammation in a mouse model |
| topic | DRNTU::Engineering::Materials::Nanostructured materials |
| url | https://hdl.handle.net/10356/105304 http://hdl.handle.net/10220/20511 |
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