3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces
Coulter counters have played an important role in biological cell assays since their introduction decades ago. Several types of high throughput micro-Coulter counters based on lab-on-chip devices have been commercialized recently. In this paper, we propose a highly integrated micro-Coulter counter a...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/105332 http://hdl.handle.net/10220/16577 http://dx.doi.org/10.1002/elps.201200418 |
| _version_ | 1824455732421984256 |
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| author | Pui, Tze Sian Guo, Jinhong Rahman, Abdur Rub Abdur Kang, Yuejun |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Pui, Tze Sian Guo, Jinhong Rahman, Abdur Rub Abdur Kang, Yuejun |
| author_sort | Pui, Tze Sian |
| collection | NTU |
| description | Coulter counters have played an important role in biological cell assays since their introduction decades ago. Several types of high throughput micro-Coulter counters based on lab-on-chip devices have been commercialized recently. In this paper, we propose a highly integrated micro-Coulter counter array working under low DC voltage. The real-time electrical current change, including the pulse amplitude and width, of the micro-Coulter counter with novel structure is systematically investigated numerically. The major types of forces exerted on the particle in the micro-Coulter counter, including hydrodynamic force and electrokinetic force are quantitatively analyzed. The simulation in this study shows the pulse profile, such as width and amplitude, is affected by both particle size and the flow condition. The special cases of multiple particle aggregation and cross-talk between neighboring channels are also considered for their effects on the electric current pulses. This simulation provides critical insight and guidance for developing next new generations of micro-Coulter counter. |
| first_indexed | 2025-02-19T03:42:53Z |
| format | Journal Article |
| id | ntu-10356/105332 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:42:53Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1053322019-12-06T21:49:17Z 3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces Pui, Tze Sian Guo, Jinhong Rahman, Abdur Rub Abdur Kang, Yuejun School of Chemical and Biomedical Engineering Coulter counters have played an important role in biological cell assays since their introduction decades ago. Several types of high throughput micro-Coulter counters based on lab-on-chip devices have been commercialized recently. In this paper, we propose a highly integrated micro-Coulter counter array working under low DC voltage. The real-time electrical current change, including the pulse amplitude and width, of the micro-Coulter counter with novel structure is systematically investigated numerically. The major types of forces exerted on the particle in the micro-Coulter counter, including hydrodynamic force and electrokinetic force are quantitatively analyzed. The simulation in this study shows the pulse profile, such as width and amplitude, is affected by both particle size and the flow condition. The special cases of multiple particle aggregation and cross-talk between neighboring channels are also considered for their effects on the electric current pulses. This simulation provides critical insight and guidance for developing next new generations of micro-Coulter counter. 2013-10-18T02:32:28Z 2019-12-06T21:49:17Z 2013-10-18T02:32:28Z 2019-12-06T21:49:17Z 2012 2012 Journal Article Guo, J., Pui, T. S., Rahman, A. R. A.,& Kang, Y. (2013). 3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces. ELECTROPHORESIS, 34(3), 417-424. 0173-0835 https://hdl.handle.net/10356/105332 http://hdl.handle.net/10220/16577 http://dx.doi.org/10.1002/elps.201200418 en ELECTROPHORESIS |
| spellingShingle | Pui, Tze Sian Guo, Jinhong Rahman, Abdur Rub Abdur Kang, Yuejun 3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces |
| title | 3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces |
| title_full | 3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces |
| title_fullStr | 3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces |
| title_full_unstemmed | 3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces |
| title_short | 3D numerical simulation of a Coulter counter array with analysis of electrokinetic forces |
| title_sort | 3d numerical simulation of a coulter counter array with analysis of electrokinetic forces |
| url | https://hdl.handle.net/10356/105332 http://hdl.handle.net/10220/16577 http://dx.doi.org/10.1002/elps.201200418 |
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