Structural model of the SARS coronavirus E channel in LMPG micelles
Coronaviruses (CoV) cause common colds in humans, but are also responsible for the recent Severe Acute, and Middle East, respiratory syndromes (SARS and MERS, respectively). A promising approach for prevention are live attenuated vaccines (LAVs), some of which target the envelope (E) protein, which...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/105338 http://hdl.handle.net/10220/48644 |
| _version_ | 1826118993677647872 |
|---|---|
| author | Surya, Wahyu Li, Yan Torres, Jaume |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Surya, Wahyu Li, Yan Torres, Jaume |
| author_sort | Surya, Wahyu |
| collection | NTU |
| description | Coronaviruses (CoV) cause common colds in humans, but are also responsible for the recent Severe Acute, and Middle East, respiratory syndromes (SARS and MERS, respectively). A promising approach for prevention are live attenuated vaccines (LAVs), some of which target the envelope (E) protein, which is a small membrane protein that forms ion channels. Unfortunately, detailed structural information is still limited for SARS-CoV E, and non-existent for other CoV E proteins. Herein, we report a structural model of a SARS-CoV E construct in LMPG micelles with, for the first time, unequivocal intermolecular NOEs. The model corresponding to the detergent-embedded region is consistent with previously obtained orientational restraints obtained in lipid bilayers and in vivo escape mutants. The C-terminal domain is mostly α-helical, and extramembrane intermolecular NOEs suggest interactions that may affect the TM channel conformation. |
| first_indexed | 2024-10-01T04:52:45Z |
| format | Journal Article |
| id | ntu-10356/105338 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:52:45Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1053382023-02-28T17:02:13Z Structural model of the SARS coronavirus E channel in LMPG micelles Surya, Wahyu Li, Yan Torres, Jaume School of Biological Sciences DRNTU::Science::Biological sciences Envelope Protein Solution NMR Coronaviruses (CoV) cause common colds in humans, but are also responsible for the recent Severe Acute, and Middle East, respiratory syndromes (SARS and MERS, respectively). A promising approach for prevention are live attenuated vaccines (LAVs), some of which target the envelope (E) protein, which is a small membrane protein that forms ion channels. Unfortunately, detailed structural information is still limited for SARS-CoV E, and non-existent for other CoV E proteins. Herein, we report a structural model of a SARS-CoV E construct in LMPG micelles with, for the first time, unequivocal intermolecular NOEs. The model corresponding to the detergent-embedded region is consistent with previously obtained orientational restraints obtained in lipid bilayers and in vivo escape mutants. The C-terminal domain is mostly α-helical, and extramembrane intermolecular NOEs suggest interactions that may affect the TM channel conformation. MOE (Min. of Education, S’pore) Accepted version 2019-06-11T09:15:41Z 2019-12-06T21:49:24Z 2019-06-11T09:15:41Z 2019-12-06T21:49:24Z 2018 Journal Article Surya, W., Li, Y., & Torres, J. (2018). Structural model of the SARS coronavirus E channel in LMPG micelles. Biochimica et Biophysica Acta - Biomembranes, 1860(6), 1309-1317. doi:10.1016/j.bbamem.2018.02.017 0005-2736 https://hdl.handle.net/10356/105338 http://hdl.handle.net/10220/48644 10.1016/j.bbamem.2018.02.017 en Biochimica et Biophysica Acta - Biomembranes © 2018 Elsevier B.V. All rights reserved. This paper was published in Biochimica et Biophysica Acta - Biomembranes and is made available with permission of Elsevier B.V. 22 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Envelope Protein Solution NMR Surya, Wahyu Li, Yan Torres, Jaume Structural model of the SARS coronavirus E channel in LMPG micelles |
| title | Structural model of the SARS coronavirus E channel in LMPG micelles |
| title_full | Structural model of the SARS coronavirus E channel in LMPG micelles |
| title_fullStr | Structural model of the SARS coronavirus E channel in LMPG micelles |
| title_full_unstemmed | Structural model of the SARS coronavirus E channel in LMPG micelles |
| title_short | Structural model of the SARS coronavirus E channel in LMPG micelles |
| title_sort | structural model of the sars coronavirus e channel in lmpg micelles |
| topic | DRNTU::Science::Biological sciences Envelope Protein Solution NMR |
| url | https://hdl.handle.net/10356/105338 http://hdl.handle.net/10220/48644 |
| work_keys_str_mv | AT suryawahyu structuralmodelofthesarscoronavirusechannelinlmpgmicelles AT liyan structuralmodelofthesarscoronavirusechannelinlmpgmicelles AT torresjaume structuralmodelofthesarscoronavirusechannelinlmpgmicelles |