C-di-GMP regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth
Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid te...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/105484 http://hdl.handle.net/10220/25959 |
| _version_ | 1826121004222513152 |
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| author | Chua, Song Lin Sivakumar, Krishnakumar Rybtke, Morten Yuan, Mingjun Andersen, Jens Bo Nielsen, Thomas Eiland Givskov, Michael Tolker-Nielsen, Tim Cao, Bin Kjelleberg, Staffan Yang, Liang |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Chua, Song Lin Sivakumar, Krishnakumar Rybtke, Morten Yuan, Mingjun Andersen, Jens Bo Nielsen, Thomas Eiland Givskov, Michael Tolker-Nielsen, Tim Cao, Bin Kjelleberg, Staffan Yang, Liang |
| author_sort | Chua, Song Lin |
| collection | NTU |
| description | Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO32–) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO32– further increased P. aeruginosa biofilm formation and resistance to TeO32–. P. aeruginosa ΔsadCΔsiaD and PAO1/plac-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO32– exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO32–. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth. |
| first_indexed | 2024-10-01T05:25:40Z |
| format | Journal Article |
| id | ntu-10356/105484 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:25:40Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1054842022-02-16T16:29:21Z C-di-GMP regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth Chua, Song Lin Sivakumar, Krishnakumar Rybtke, Morten Yuan, Mingjun Andersen, Jens Bo Nielsen, Thomas Eiland Givskov, Michael Tolker-Nielsen, Tim Cao, Bin Kjelleberg, Staffan Yang, Liang School of Civil and Environmental Engineering School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Microbiology::Microorganisms Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO32–) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO32– further increased P. aeruginosa biofilm formation and resistance to TeO32–. P. aeruginosa ΔsadCΔsiaD and PAO1/plac-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO32– exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO32–. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth. Published version 2015-06-18T04:09:34Z 2019-12-06T21:52:12Z 2015-06-18T04:09:34Z 2019-12-06T21:52:12Z 2015 2015 Journal Article Chua, S. L., Sivakumar, K., Rybtke, M., Yuan, M., Andersen, J. B., Nielsen, et al. (2015). C-di-GMP regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth. Scientific Reports, 5, 10052-. 2045-2322 https://hdl.handle.net/10356/105484 http://hdl.handle.net/10220/25959 10.1038/srep10052 25992876 en Scientific Reports This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Microorganisms Chua, Song Lin Sivakumar, Krishnakumar Rybtke, Morten Yuan, Mingjun Andersen, Jens Bo Nielsen, Thomas Eiland Givskov, Michael Tolker-Nielsen, Tim Cao, Bin Kjelleberg, Staffan Yang, Liang C-di-GMP regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth |
| title | C-di-GMP regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth |
| title_full | C-di-GMP regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth |
| title_fullStr | C-di-GMP regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth |
| title_full_unstemmed | C-di-GMP regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth |
| title_short | C-di-GMP regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth |
| title_sort | c di gmp regulates pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth |
| topic | DRNTU::Science::Biological sciences::Microbiology::Microorganisms |
| url | https://hdl.handle.net/10356/105484 http://hdl.handle.net/10220/25959 |
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