Contact guidance for cardiac tissue engineering using 3D bioprinted gelatin patterned hydrogel
Here, we have developed a 3D bioprinted microchanneled gelatin hydrogel that promotes human mesenchymal stem cell (hMSC) myocardial commitment and supports native cardiomyocytes(CMs) contractile functionality. Firstly, we studied the effect of bioprinted microchanneled hydrogel on the alignment,...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/105586 http://hdl.handle.net/10220/49539 |
| Summary: | Here, we have developed a 3D bioprinted microchanneled gelatin hydrogel that promotes human
mesenchymal stem cell (hMSC) myocardial commitment and supports native cardiomyocytes(CMs)
contractile functionality. Firstly, we studied the effect of bioprinted microchanneled hydrogel on the
alignment, elongation, and differentiation of hMSC. Notably, the cells displayed well defined F-actin
anisotropy and elongated morphology on the microchanneled hydrogel, hence showing the effects of topographical control over cell behavior. Furthermore, the aligned stem cells showed myocardial
lineage commitment, as detected using mature cardiac markers. The fluorescence-activated cell
sorting analysis also confirmed a significant increase in the commitment towards myocardial tissue
lineage. Moreover, seeded CMs were found to be more aligned and demonstrated synchronized
beating on microchanneled hydrogel as compared to the unpatterned hydrogel. Overall, our study
proved that microchanneled hydrogel scaffold produced by 3D bioprinting induces myocardial
differentiation of stem cells as well as supports CMs growth and contractility. Applications of this
approach may be beneficial for generating in vitro cardiac model systems to physiological and
cardiotoxicity studies as well asin vivo generating custom designed cell impregnated constructs for
tissue engineering and regenerative medicine applications. |
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