SERS biosensor using metallic nano-sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin
A biosensor chip is developed for the detection of a protein biomarker of endocrine disrupting compounds, vitellogenin (Vg) in aquatic environment. The sensor chip is fabricated by immobilizing anti-Vg antibody on 4-Aminothiophenol (4-ATP) coated nanosculptured thin films (nSTFs) of silver on Si sub...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/105773 http://hdl.handle.net/10220/20911 |
| _version_ | 1826111746292580352 |
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| author | Srivastava, Sachin K. Shalabney, Atef Khalaila, Isam Grüner, Christoph Rauschenbach, Bernd Abdulhalim, Ibrahim |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Srivastava, Sachin K. Shalabney, Atef Khalaila, Isam Grüner, Christoph Rauschenbach, Bernd Abdulhalim, Ibrahim |
| author_sort | Srivastava, Sachin K. |
| collection | NTU |
| description | A biosensor chip is developed for the detection of a protein biomarker of endocrine disrupting compounds, vitellogenin (Vg) in aquatic environment. The sensor chip is fabricated by immobilizing anti-Vg antibody on 4-Aminothiophenol (4-ATP) coated nanosculptured thin films (nSTFs) of silver on Si substrates. The biosensor is based on the SERS of 4-ATP, enhanced by the Ag nSTFs. Before the fabrication of the sensor, the performance of the enhancement is optimized with respect to the porosity of nSTFs. Further, the biosensor is developed on the nSTF with optimized enhancement. The SERS signals are recorded from the sensor chip for varying concentrations of Vg. A control experiment is performed on another similar protein Fetuin to confirm the specificity of the sensor. The repeatability and reusability of the sensor, along with its shelf life are also checked. The limit of detection of the sensor is found to be 5 pg mL-1 of Vg in PBS within our experimental window. Apart from high sensitivity, specificity and reusability, the present sensor provides additional advantages of miniaturization, requirement of very small volumes of the analyte solution (15 μL) and fast response as compared to conventional techniques e.g., ELISA, as its response time is less than 3 minutes. |
| first_indexed | 2024-10-01T02:55:31Z |
| format | Journal Article |
| id | ntu-10356/105773 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:55:31Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1057732020-06-01T10:21:29Z SERS biosensor using metallic nano-sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin Srivastava, Sachin K. Shalabney, Atef Khalaila, Isam Grüner, Christoph Rauschenbach, Bernd Abdulhalim, Ibrahim School of Materials Science & Engineering DRNTU::Science::Medicine::Biosensors DRNTU::Engineering::Materials::Biomaterials A biosensor chip is developed for the detection of a protein biomarker of endocrine disrupting compounds, vitellogenin (Vg) in aquatic environment. The sensor chip is fabricated by immobilizing anti-Vg antibody on 4-Aminothiophenol (4-ATP) coated nanosculptured thin films (nSTFs) of silver on Si substrates. The biosensor is based on the SERS of 4-ATP, enhanced by the Ag nSTFs. Before the fabrication of the sensor, the performance of the enhancement is optimized with respect to the porosity of nSTFs. Further, the biosensor is developed on the nSTF with optimized enhancement. The SERS signals are recorded from the sensor chip for varying concentrations of Vg. A control experiment is performed on another similar protein Fetuin to confirm the specificity of the sensor. The repeatability and reusability of the sensor, along with its shelf life are also checked. The limit of detection of the sensor is found to be 5 pg mL-1 of Vg in PBS within our experimental window. Apart from high sensitivity, specificity and reusability, the present sensor provides additional advantages of miniaturization, requirement of very small volumes of the analyte solution (15 μL) and fast response as compared to conventional techniques e.g., ELISA, as its response time is less than 3 minutes. 2014-09-19T03:21:08Z 2019-12-06T21:57:34Z 2014-09-19T03:21:08Z 2019-12-06T21:57:34Z 2014 2014 Journal Article Srivastava, S. K., Shalabney, A., Khalaila, I., Grüner, C., Rauschenbach, B., & Abdulhalim, I. (2014). SERS biosensor using metallic nano-sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin. Small, 10(17), 3579-3587. 1613-6810 https://hdl.handle.net/10356/105773 http://hdl.handle.net/10220/20911 10.1002/smll.201303218 en Small © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Science::Medicine::Biosensors DRNTU::Engineering::Materials::Biomaterials Srivastava, Sachin K. Shalabney, Atef Khalaila, Isam Grüner, Christoph Rauschenbach, Bernd Abdulhalim, Ibrahim SERS biosensor using metallic nano-sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin |
| title | SERS biosensor using metallic nano-sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin |
| title_full | SERS biosensor using metallic nano-sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin |
| title_fullStr | SERS biosensor using metallic nano-sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin |
| title_full_unstemmed | SERS biosensor using metallic nano-sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin |
| title_short | SERS biosensor using metallic nano-sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin |
| title_sort | sers biosensor using metallic nano sculptured thin films for the detection of endocrine disrupting compound biomarker vitellogenin |
| topic | DRNTU::Science::Medicine::Biosensors DRNTU::Engineering::Materials::Biomaterials |
| url | https://hdl.handle.net/10356/105773 http://hdl.handle.net/10220/20911 |
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