Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore
With 70% of the Earth’s surface covered in water, the marine ecosystem offers immense opportunities for drug discovery and development. Due to the decreasing rate of novel natural product discovery from terrestrial sources in recent years, many researchers are beginning to look seaward for breakthro...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/105967 http://hdl.handle.net/10220/48802 |
| _version_ | 1826121698698592256 |
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| author | Chin, Joyce Seow Fong Tan, Koh Siang Goh, Hui Chin Lim, Swee Cheng Pang, Li Mei Gerwick, William H. Tan, Lik Tong Liang, Zhao-Xun Yang, Liang Glukhov, Evgenia Ong, Marshall Ji Fa |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Chin, Joyce Seow Fong Tan, Koh Siang Goh, Hui Chin Lim, Swee Cheng Pang, Li Mei Gerwick, William H. Tan, Lik Tong Liang, Zhao-Xun Yang, Liang Glukhov, Evgenia Ong, Marshall Ji Fa |
| author_sort | Chin, Joyce Seow Fong |
| collection | NTU |
| description | With 70% of the Earth’s surface covered in water, the marine ecosystem offers immense opportunities for drug discovery and development. Due to the decreasing rate of novel natural product discovery from terrestrial sources in recent years, many researchers are beginning to look seaward for breakthroughs in new therapeutic agents. As part of an ongoing marine drug discovery programme in Singapore, an integrated approach of combining metabolomic and genomic techniques were initiated for uncovering novel anti-quorum sensing molecules from bacteria associated with subtidal samples collected in the Singapore Strait. Based on the culture-dependent method, a total of 102 marine bacteria strains were isolated and the identities of selected strains were established based on their 16S rRNA gene sequences. About 5% of the marine bacterial organic extracts showed quorum sensing inhibitory (QSI) activity in a dose-dependent manner based on the Pseudomonas aeruginosa QS reporter system. In addition, the extracts were subjected to mass spectrometry-based molecular networking and the genome of selected strains were analysed for known as well as new biosynthetic gene clusters. This study revealed that using integrated techniques, coupled with biological assays, can provide an effective and rapid prioritization of marine bacterial strains for downstream large-scale culturing for the purpose of isolation and structural elucidation of novel bioactive compounds. |
| first_indexed | 2024-10-01T05:36:46Z |
| format | Journal Article |
| id | ntu-10356/105967 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:36:46Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1059672020-09-21T11:34:03Z Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore Chin, Joyce Seow Fong Tan, Koh Siang Goh, Hui Chin Lim, Swee Cheng Pang, Li Mei Gerwick, William H. Tan, Lik Tong Liang, Zhao-Xun Yang, Liang Glukhov, Evgenia Ong, Marshall Ji Fa School of Biological Sciences National Institute of Education Singapore Centre for Environmental Life Sciences and Engineering Marine Bacteria DRNTU::Science::Biological sciences Marine Sponges With 70% of the Earth’s surface covered in water, the marine ecosystem offers immense opportunities for drug discovery and development. Due to the decreasing rate of novel natural product discovery from terrestrial sources in recent years, many researchers are beginning to look seaward for breakthroughs in new therapeutic agents. As part of an ongoing marine drug discovery programme in Singapore, an integrated approach of combining metabolomic and genomic techniques were initiated for uncovering novel anti-quorum sensing molecules from bacteria associated with subtidal samples collected in the Singapore Strait. Based on the culture-dependent method, a total of 102 marine bacteria strains were isolated and the identities of selected strains were established based on their 16S rRNA gene sequences. About 5% of the marine bacterial organic extracts showed quorum sensing inhibitory (QSI) activity in a dose-dependent manner based on the Pseudomonas aeruginosa QS reporter system. In addition, the extracts were subjected to mass spectrometry-based molecular networking and the genome of selected strains were analysed for known as well as new biosynthetic gene clusters. This study revealed that using integrated techniques, coupled with biological assays, can provide an effective and rapid prioritization of marine bacterial strains for downstream large-scale culturing for the purpose of isolation and structural elucidation of novel bioactive compounds. NRF (Natl Research Foundation, S’pore) Published version 2019-06-18T07:21:02Z 2019-12-06T22:01:49Z 2019-06-18T07:21:02Z 2019-12-06T22:01:49Z 2019 Journal Article Ong, M. J. F., Goh, H. C., Lim, S. C., Pang, L. M., Chin, J. S. F., Tan, K. S., . . . Tan, L. T. (2019). Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore. Marine Drugs, 17(1), 72-. doi:10.3390/md17010072 1660-3397 https://hdl.handle.net/10356/105967 http://hdl.handle.net/10220/48802 10.3390/md17010072 en Marine Drugs © 2019 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). 15 p. application/pdf |
| spellingShingle | Marine Bacteria DRNTU::Science::Biological sciences Marine Sponges Chin, Joyce Seow Fong Tan, Koh Siang Goh, Hui Chin Lim, Swee Cheng Pang, Li Mei Gerwick, William H. Tan, Lik Tong Liang, Zhao-Xun Yang, Liang Glukhov, Evgenia Ong, Marshall Ji Fa Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore |
| title | Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore |
| title_full | Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore |
| title_fullStr | Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore |
| title_full_unstemmed | Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore |
| title_short | Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore |
| title_sort | integrated genomic and metabolomic approach to the discovery of potential anti quorum sensing natural products from microbes associated with marine samples from singapore |
| topic | Marine Bacteria DRNTU::Science::Biological sciences Marine Sponges |
| url | https://hdl.handle.net/10356/105967 http://hdl.handle.net/10220/48802 |
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