Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip
A microfluidic chip integrated with pneumatically controlled valves was developed for multiplexed biomolecular detection via localized surface plasmonic resonance (LSPR) of single gold nanorod. The cost-effective microfluidic chip was assembled by polydimethylsiloxane (PDMS) layers and glass substra...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/106187 http://hdl.handle.net/10220/24981 http://dx.doi.org/10.1007/s11468-013-9661-4 |
| _version_ | 1811691978836934656 |
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| author | Wu, Bo Chen, Li-Chan Huang, Youju Zhang, Yiming Kang, Yuejun Kim, Dong-Hwan |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Wu, Bo Chen, Li-Chan Huang, Youju Zhang, Yiming Kang, Yuejun Kim, Dong-Hwan |
| author_sort | Wu, Bo |
| collection | NTU |
| description | A microfluidic chip integrated with pneumatically controlled valves was developed for multiplexed biomolecular detection via localized surface plasmonic resonance (LSPR) of single gold nanorod. The cost-effective microfluidic chip was assembled by polydimethylsiloxane (PDMS) layers and glass substrates with a precisely controlled thickness. The thin and flat microfluidic chip fitted the narrow space of dark-field microscopy and enabled the recording of single nanoparticle LSPR responses. Aptamer-antigen-antibody sandwiched detection scheme was utilized to enhance the LSPR responses for label-free biomolecular detection. This microfluidic chip successfully demonstrated the multiplexed detection of three independent analytes (PSA, IgE, and thrombin). |
| first_indexed | 2024-10-01T06:28:29Z |
| format | Journal Article |
| id | ntu-10356/106187 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:28:29Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1061872019-12-06T22:06:00Z Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip Wu, Bo Chen, Li-Chan Huang, Youju Zhang, Yiming Kang, Yuejun Kim, Dong-Hwan School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biochemical engineering A microfluidic chip integrated with pneumatically controlled valves was developed for multiplexed biomolecular detection via localized surface plasmonic resonance (LSPR) of single gold nanorod. The cost-effective microfluidic chip was assembled by polydimethylsiloxane (PDMS) layers and glass substrates with a precisely controlled thickness. The thin and flat microfluidic chip fitted the narrow space of dark-field microscopy and enabled the recording of single nanoparticle LSPR responses. Aptamer-antigen-antibody sandwiched detection scheme was utilized to enhance the LSPR responses for label-free biomolecular detection. This microfluidic chip successfully demonstrated the multiplexed detection of three independent analytes (PSA, IgE, and thrombin). Accepted version 2015-01-26T02:43:42Z 2019-12-06T22:06:00Z 2015-01-26T02:43:42Z 2019-12-06T22:06:00Z 2014 2014 Journal Article Wu, B., Chen, L. C., Huang, Y., Zhang, Y., Kang, Y., & Kim, D. H. (2014). Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip. Plasmonics, 9(4), 801-807. https://hdl.handle.net/10356/106187 http://hdl.handle.net/10220/24981 http://dx.doi.org/10.1007/s11468-013-9661-4 en Plasmonics © 2014 Springer Science+Business Media New York. This is the author created version of a work that has been peer reviewed and accepted for publication by Plasmonics, Springer Science+Business Media New York. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [Article DOI: http://dx.doi.org/10.1007/s11468-013-9661-4]. 17 p. application/msword application/msword |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biochemical engineering Wu, Bo Chen, Li-Chan Huang, Youju Zhang, Yiming Kang, Yuejun Kim, Dong-Hwan Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip |
| title | Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip |
| title_full | Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip |
| title_fullStr | Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip |
| title_full_unstemmed | Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip |
| title_short | Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip |
| title_sort | multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip |
| topic | DRNTU::Engineering::Chemical engineering::Biochemical engineering |
| url | https://hdl.handle.net/10356/106187 http://hdl.handle.net/10220/24981 http://dx.doi.org/10.1007/s11468-013-9661-4 |
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