Inhibitory effects of salviae miltiorrhizae radix (danshen) and puerariae lobatae radix (gegen) in carbachol-induced rat detrusor smooth muscle contractility
Both danshen (D) and gegen (G) have proven relaxant effects on vascular smooth muscle, thus their potential bladder inhibitory effects have impending interests in urology. The aim of this study was to demonstrate the novel effects of D and G on detrusor smooth muscle contractility. Urothelium-in...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/106238 http://hdl.handle.net/10220/23957 http://www.ijppp.org/1201001A.html |
| _version_ | 1826114583995088896 |
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| author | Teong, Ivy Wen Jia Koon, Johnny Chi Man Lau, Clara Bik San Liang, Willmann Leung, Ping Chung Fung, Kwok Pui |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Teong, Ivy Wen Jia Koon, Johnny Chi Man Lau, Clara Bik San Liang, Willmann Leung, Ping Chung Fung, Kwok Pui |
| author_sort | Teong, Ivy Wen Jia |
| collection | NTU |
| description | Both danshen (D) and gegen (G) have proven relaxant effects on vascular smooth muscle, thus their potential bladder
inhibitory effects have impending interests in urology. The aim of this study was to demonstrate the novel effects of D and G on detrusor
smooth muscle contractility. Urothelium-intact (+UE) and urothelium-denuded (–UE) detrusor strips were isolated from the rat.
Isometric tension was measured using a myograph system. Carbachol (CCh) was used to pre-contract the detrusor strips prior to
stepwise relaxation by adding extracts of D, G, and a DG (7:3) formulation. Tonic relaxation level and phasic contractile activity under the
herbal treatments were analyzed. There was no difference in the herbal effects between +UE and –UE strips. D alone induced a much
smaller relaxation than G alone or DG. G alone also suppressed phasic amplitude but not phasic frequency while DG suppressed both
parameters. D and G acted synergistically to yield the observed effects on detrusor smooth muscle. The findings showed that the DG
formulation were able to relax the detrusor as well as suppress phasic contractions, both actions important in maintaining normal
bladder filling and urine storage processes. Hence DG may have new application in the management of bladder disorders. |
| first_indexed | 2024-10-01T03:41:31Z |
| format | Journal Article |
| id | ntu-10356/106238 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:41:31Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1062382023-02-28T17:06:51Z Inhibitory effects of salviae miltiorrhizae radix (danshen) and puerariae lobatae radix (gegen) in carbachol-induced rat detrusor smooth muscle contractility Teong, Ivy Wen Jia Koon, Johnny Chi Man Lau, Clara Bik San Liang, Willmann Leung, Ping Chung Fung, Kwok Pui School of Biological Sciences DRNTU::Science::Biological sciences Both danshen (D) and gegen (G) have proven relaxant effects on vascular smooth muscle, thus their potential bladder inhibitory effects have impending interests in urology. The aim of this study was to demonstrate the novel effects of D and G on detrusor smooth muscle contractility. Urothelium-intact (+UE) and urothelium-denuded (–UE) detrusor strips were isolated from the rat. Isometric tension was measured using a myograph system. Carbachol (CCh) was used to pre-contract the detrusor strips prior to stepwise relaxation by adding extracts of D, G, and a DG (7:3) formulation. Tonic relaxation level and phasic contractile activity under the herbal treatments were analyzed. There was no difference in the herbal effects between +UE and –UE strips. D alone induced a much smaller relaxation than G alone or DG. G alone also suppressed phasic amplitude but not phasic frequency while DG suppressed both parameters. D and G acted synergistically to yield the observed effects on detrusor smooth muscle. The findings showed that the DG formulation were able to relax the detrusor as well as suppress phasic contractions, both actions important in maintaining normal bladder filling and urine storage processes. Hence DG may have new application in the management of bladder disorders. Published version 2014-10-03T08:36:02Z 2019-12-06T22:07:08Z 2014-10-03T08:36:02Z 2019-12-06T22:07:08Z 2012 2012 Journal Article Liang, W., Teong, I. W. J., Koon, J. C. M., Lau, C. B. S., Fung, K. P., & Leung, P. C. (2012). Inhibitory effects of salviae miltiorrhizae radix (danshen) and puerariae lobatae radix (gegen) in carbachol-induced rat detrusor smooth muscle contractility. International journal of physiology, pathophysiology and pharmacology, 4(1), 36-44. 1944-8171 https://hdl.handle.net/10356/106238 http://hdl.handle.net/10220/23957 http://www.ijppp.org/1201001A.html en International journal of physiology, pathophysiology and pharmacology © 2012 e-Century Publishing. This paper was published in International Journal of Physiology, Pathophysiology and Pharmacology and is made available as an electronic reprint (preprint) with permission of e-Century Publishing. The paper can be found at the following official URL: http://www.ijppp.org/1201001A.html. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 9 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Teong, Ivy Wen Jia Koon, Johnny Chi Man Lau, Clara Bik San Liang, Willmann Leung, Ping Chung Fung, Kwok Pui Inhibitory effects of salviae miltiorrhizae radix (danshen) and puerariae lobatae radix (gegen) in carbachol-induced rat detrusor smooth muscle contractility |
| title | Inhibitory effects of salviae miltiorrhizae radix (danshen) and puerariae lobatae radix (gegen) in carbachol-induced rat detrusor smooth muscle contractility |
| title_full | Inhibitory effects of salviae miltiorrhizae radix (danshen) and puerariae lobatae radix (gegen) in carbachol-induced rat detrusor smooth muscle contractility |
| title_fullStr | Inhibitory effects of salviae miltiorrhizae radix (danshen) and puerariae lobatae radix (gegen) in carbachol-induced rat detrusor smooth muscle contractility |
| title_full_unstemmed | Inhibitory effects of salviae miltiorrhizae radix (danshen) and puerariae lobatae radix (gegen) in carbachol-induced rat detrusor smooth muscle contractility |
| title_short | Inhibitory effects of salviae miltiorrhizae radix (danshen) and puerariae lobatae radix (gegen) in carbachol-induced rat detrusor smooth muscle contractility |
| title_sort | inhibitory effects of salviae miltiorrhizae radix danshen and puerariae lobatae radix gegen in carbachol induced rat detrusor smooth muscle contractility |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/106238 http://hdl.handle.net/10220/23957 http://www.ijppp.org/1201001A.html |
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