Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface
The heme protein cytochrome c adsorbed to a two-layer gold surface modified with a self-assembled monolayer of 2-mercaptoethanol was analyzed using a two-dimensional (2D) heterospectral correlation analysis that combined surface-enhanced infrared absorption spectroscopy (SEIRAS) and surface-enhanced...
| Κύριοι συγγραφείς: | , , , , , , , , |
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| Άλλοι συγγραφείς: | |
| Μορφή: | Journal Article |
| Γλώσσα: | English |
| Έκδοση: |
2013
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| Θέματα: | |
| Διαθέσιμο Online: | https://hdl.handle.net/10356/106243 http://hdl.handle.net/10220/17762 |
| _version_ | 1826130726353895424 |
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| author | Zou, Changji Larisika, Melanie Nagy, Gabor Srajer, Johannes Oostenbrink, Chris Chen, Xiaodong Knoll, Wolfgang Nowak, Christoph Bo, Liedberg |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Zou, Changji Larisika, Melanie Nagy, Gabor Srajer, Johannes Oostenbrink, Chris Chen, Xiaodong Knoll, Wolfgang Nowak, Christoph Bo, Liedberg |
| author_sort | Zou, Changji |
| collection | NTU |
| description | The heme protein cytochrome c adsorbed to a two-layer gold surface modified with a self-assembled monolayer of 2-mercaptoethanol was analyzed using a two-dimensional (2D) heterospectral correlation analysis that combined surface-enhanced infrared absorption spectroscopy (SEIRAS) and surface-enhanced Raman spectroscopy (SERS). Stepwise increasing electric potentials were applied to alter the redox state of the protein and to induce conformational changes within the protein backbone. We demonstrate herein that 2D heterospectral correlation analysis is a particularly suitable and useful technique for the study of heme-containing proteins as the two spectroscopies address different portions of the protein. Thus, by correlating SERS and SEIRAS data in a 2D plot, we can obtain a deeper understanding of the conformational changes occurring at the redox center and in the supporting protein backbone during the electron transfer process. The correlation analyses are complemented by molecular dynamics calculations to explore the intramolecular interactions. |
| first_indexed | 2024-10-01T08:00:36Z |
| format | Journal Article |
| id | ntu-10356/106243 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T08:00:36Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1062432023-07-14T15:58:00Z Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface Zou, Changji Larisika, Melanie Nagy, Gabor Srajer, Johannes Oostenbrink, Chris Chen, Xiaodong Knoll, Wolfgang Nowak, Christoph Bo, Liedberg School of Materials Science & Engineering surface-enhanced Raman spectroscopy redox protein Two-layer gold surface 2D heterospectral correlation spectroscopy cytochrome c surface-enhanced infrared absorption spectroscopy DRNTU::Engineering::Materials The heme protein cytochrome c adsorbed to a two-layer gold surface modified with a self-assembled monolayer of 2-mercaptoethanol was analyzed using a two-dimensional (2D) heterospectral correlation analysis that combined surface-enhanced infrared absorption spectroscopy (SEIRAS) and surface-enhanced Raman spectroscopy (SERS). Stepwise increasing electric potentials were applied to alter the redox state of the protein and to induce conformational changes within the protein backbone. We demonstrate herein that 2D heterospectral correlation analysis is a particularly suitable and useful technique for the study of heme-containing proteins as the two spectroscopies address different portions of the protein. Thus, by correlating SERS and SEIRAS data in a 2D plot, we can obtain a deeper understanding of the conformational changes occurring at the redox center and in the supporting protein backbone during the electron transfer process. The correlation analyses are complemented by molecular dynamics calculations to explore the intramolecular interactions. ASTAR (Agency for Sci., Tech. and Research, S’pore) Accepted version 2013-11-15T08:26:36Z 2019-12-06T22:07:13Z 2013-11-15T08:26:36Z 2019-12-06T22:07:13Z 2013 2013 Journal Article Zou, C., Larisika, M., Nagy, G., Srajer, J., Oostenbrink, C., Chen, X., et al. (2013). Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced raman and infrared absorption spectroscopies on a two-layer gold surface. The journal of physical chemistry B, 117(33), 9606-9614. https://hdl.handle.net/10356/106243 http://hdl.handle.net/10220/17762 10.1021/jp404573q 23930980 en The journal of physical chemistry B © 2013 American Chemical Society (ACS). This is the author created version of a work that has been peer reviewed and accepted for publication by The Journal of Physical Chemistry B, American Chemical Society (ACS). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/jp404573q]. application/pdf |
| spellingShingle | surface-enhanced Raman spectroscopy redox protein Two-layer gold surface 2D heterospectral correlation spectroscopy cytochrome c surface-enhanced infrared absorption spectroscopy DRNTU::Engineering::Materials Zou, Changji Larisika, Melanie Nagy, Gabor Srajer, Johannes Oostenbrink, Chris Chen, Xiaodong Knoll, Wolfgang Nowak, Christoph Bo, Liedberg Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface |
| title | Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface |
| title_full | Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface |
| title_fullStr | Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface |
| title_full_unstemmed | Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface |
| title_short | Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface |
| title_sort | two dimensional heterospectral correlation analysis of the redox induced conformational transition in cytochrome c using surface enhanced raman and infrared absorption spectroscopies on a two layer gold surface |
| topic | surface-enhanced Raman spectroscopy redox protein Two-layer gold surface 2D heterospectral correlation spectroscopy cytochrome c surface-enhanced infrared absorption spectroscopy DRNTU::Engineering::Materials |
| url | https://hdl.handle.net/10356/106243 http://hdl.handle.net/10220/17762 |
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