Dynamics of actin waves on patterned substrates : a quantitative analysis of circular dorsal ruffles
Circular Dorsal Ruffles (CDRs) have been known for decades, but the mechanism that organizes these actin waves remains unclear. In this article we systematically analyze the dynamics of CDRs on fibroblasts with respect to characteristics of current models of actin waves. We studied CDRs on heterogen...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/106533 http://hdl.handle.net/10220/24999 |
| _version_ | 1811680739229433856 |
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| author | Bernitt, Erik Koh, Cheng Gee Gov, Nir Döbereiner, Hans-Günther |
| author2 | Nabi, Ivan R |
| author_facet | Nabi, Ivan R Bernitt, Erik Koh, Cheng Gee Gov, Nir Döbereiner, Hans-Günther |
| author_sort | Bernitt, Erik |
| collection | NTU |
| description | Circular Dorsal Ruffles (CDRs) have been known for decades, but the mechanism that organizes these actin waves remains unclear. In this article we systematically analyze the dynamics of CDRs on fibroblasts with respect to characteristics of current models of actin waves. We studied CDRs on heterogeneously shaped cells and on cells that we forced into disk-like morphology. We show that CDRs exhibit phenomena such as periodic cycles of formation, spiral patterns, and mutual wave annihilations that are in accord with an active medium description of CDRs. On cells of controlled morphologies, CDRs exhibit extremely regular patterns of repeated wave formation and propagation, whereas on random-shaped cells the dynamics seem to be dominated by the limited availability of a reactive species. We show that theoretical models of reaction-diffusion type incorporating conserved species capture partially the behavior we observe in our data. |
| first_indexed | 2024-10-01T03:29:50Z |
| format | Journal Article |
| id | ntu-10356/106533 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:29:50Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1065332023-02-28T17:07:43Z Dynamics of actin waves on patterned substrates : a quantitative analysis of circular dorsal ruffles Bernitt, Erik Koh, Cheng Gee Gov, Nir Döbereiner, Hans-Günther Nabi, Ivan R School of Biological Sciences DRNTU::Science::Biological sciences Circular Dorsal Ruffles (CDRs) have been known for decades, but the mechanism that organizes these actin waves remains unclear. In this article we systematically analyze the dynamics of CDRs on fibroblasts with respect to characteristics of current models of actin waves. We studied CDRs on heterogeneously shaped cells and on cells that we forced into disk-like morphology. We show that CDRs exhibit phenomena such as periodic cycles of formation, spiral patterns, and mutual wave annihilations that are in accord with an active medium description of CDRs. On cells of controlled morphologies, CDRs exhibit extremely regular patterns of repeated wave formation and propagation, whereas on random-shaped cells the dynamics seem to be dominated by the limited availability of a reactive species. We show that theoretical models of reaction-diffusion type incorporating conserved species capture partially the behavior we observe in our data. Published version 2015-02-02T04:03:00Z 2019-12-06T22:13:35Z 2015-02-02T04:03:00Z 2019-12-06T22:13:35Z 2015 2015 Journal Article Bernitt, E., Koh, C. G., Gov, N., & Döbereiner, H.-G. (2015). Dynamics of actin waves on patterned substrates : a quantitative analysis of circular dorsal ruffles. PLOS One, 10(1), e0115857-. 1932-6203 https://hdl.handle.net/10356/106533 http://hdl.handle.net/10220/24999 10.1371/journal.pone.0115857 25574668 en PLOS One © 2015 Bernitt et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Bernitt, Erik Koh, Cheng Gee Gov, Nir Döbereiner, Hans-Günther Dynamics of actin waves on patterned substrates : a quantitative analysis of circular dorsal ruffles |
| title | Dynamics of actin waves on patterned substrates : a quantitative analysis of circular dorsal ruffles |
| title_full | Dynamics of actin waves on patterned substrates : a quantitative analysis of circular dorsal ruffles |
| title_fullStr | Dynamics of actin waves on patterned substrates : a quantitative analysis of circular dorsal ruffles |
| title_full_unstemmed | Dynamics of actin waves on patterned substrates : a quantitative analysis of circular dorsal ruffles |
| title_short | Dynamics of actin waves on patterned substrates : a quantitative analysis of circular dorsal ruffles |
| title_sort | dynamics of actin waves on patterned substrates a quantitative analysis of circular dorsal ruffles |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/106533 http://hdl.handle.net/10220/24999 |
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