POPX2 phosphatase regulates the KIF3 kinesin motor complex
The kinesin motors are important in the regulation of cellular functions such as protein trafficking, spindle organization and centrosome separation. In this study, we have identified POPX2, a serine-threonine phosphatase, as an interacting partner of the KAP3 subunit of the kinesin-2 motor. The kin...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/106722 http://hdl.handle.net/10220/25111 |
| _version_ | 1826125751957585920 |
|---|---|
| author | Phang, Hui-Qun Hoon, Jing-Ling Lai, Soak-Kuan Zeng, Yukai Chiam, Keng-Hwee Li, Hoi-Yeung Koh, Cheng-Gee |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Phang, Hui-Qun Hoon, Jing-Ling Lai, Soak-Kuan Zeng, Yukai Chiam, Keng-Hwee Li, Hoi-Yeung Koh, Cheng-Gee |
| author_sort | Phang, Hui-Qun |
| collection | NTU |
| description | The kinesin motors are important in the regulation of cellular functions such as protein trafficking, spindle organization and centrosome separation. In this study, we have identified POPX2, a serine-threonine phosphatase, as an interacting partner of the KAP3 subunit of the kinesin-2 motor. The kinesin-2 motor is a heterotrimeric complex composed of KIF3A, KIF3B motor subunits and KAP3, the non-motor subunit, which binds the cargo. Here we report that the phosphatase POPX2 is a negative regulator of the trafficking of N-cadherin and other cargoes; consequently, it markedly influences cell-cell adhesion. POPX2 affects trafficking by determining the phosphorylation status of KIF3A at serine 690. This is consistent with the observation that the KIF3A-S690A mutant is defective in cargo trafficking. Our studies also implicate CaMKII as the kinase that phosphorylates KIF3A at serine 690. These results strongly suggest that POPX2 and CaMKII are a phosphatase-kinase pair that regulates kinesin-mediated transport and cell-cell adhesion. |
| first_indexed | 2024-10-01T06:41:48Z |
| format | Journal Article |
| id | ntu-10356/106722 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:41:48Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1067222023-02-28T17:05:26Z POPX2 phosphatase regulates the KIF3 kinesin motor complex Phang, Hui-Qun Hoon, Jing-Ling Lai, Soak-Kuan Zeng, Yukai Chiam, Keng-Hwee Li, Hoi-Yeung Koh, Cheng-Gee School of Biological Sciences DRNTU::Science::Biological sciences The kinesin motors are important in the regulation of cellular functions such as protein trafficking, spindle organization and centrosome separation. In this study, we have identified POPX2, a serine-threonine phosphatase, as an interacting partner of the KAP3 subunit of the kinesin-2 motor. The kinesin-2 motor is a heterotrimeric complex composed of KIF3A, KIF3B motor subunits and KAP3, the non-motor subunit, which binds the cargo. Here we report that the phosphatase POPX2 is a negative regulator of the trafficking of N-cadherin and other cargoes; consequently, it markedly influences cell-cell adhesion. POPX2 affects trafficking by determining the phosphorylation status of KIF3A at serine 690. This is consistent with the observation that the KIF3A-S690A mutant is defective in cargo trafficking. Our studies also implicate CaMKII as the kinase that phosphorylates KIF3A at serine 690. These results strongly suggest that POPX2 and CaMKII are a phosphatase-kinase pair that regulates kinesin-mediated transport and cell-cell adhesion. Accepted version 2015-02-26T04:07:54Z 2019-12-06T22:16:57Z 2015-02-26T04:07:54Z 2019-12-06T22:16:57Z 2014 2014 Journal Article Phang, H.-Q., Hoon, J.-L., Lai, S.-K., Zeng, Y., Chiam, K.-H., Li, H.-Y., et al. (2014). POPX2 phosphatase regulates the KIF3 kinesin motor complex. Journal of cell science, 127(4), 727-739. https://hdl.handle.net/10356/106722 http://hdl.handle.net/10220/25111 10.1242/jcs.126482 en Journal of cell science © The Authors (published by The Company of Biologists Ltd.). This is the author created version of a work that has been peer reviewed and accepted for publication in Journal of Cell Science, published by The Company of Biologists Ltd. on behalf of The Authors. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [Article DOI: http://dx.doi.org/10.1242/jcs.126482]. 36 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Phang, Hui-Qun Hoon, Jing-Ling Lai, Soak-Kuan Zeng, Yukai Chiam, Keng-Hwee Li, Hoi-Yeung Koh, Cheng-Gee POPX2 phosphatase regulates the KIF3 kinesin motor complex |
| title | POPX2 phosphatase regulates the KIF3 kinesin motor complex |
| title_full | POPX2 phosphatase regulates the KIF3 kinesin motor complex |
| title_fullStr | POPX2 phosphatase regulates the KIF3 kinesin motor complex |
| title_full_unstemmed | POPX2 phosphatase regulates the KIF3 kinesin motor complex |
| title_short | POPX2 phosphatase regulates the KIF3 kinesin motor complex |
| title_sort | popx2 phosphatase regulates the kif3 kinesin motor complex |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/106722 http://hdl.handle.net/10220/25111 |
| work_keys_str_mv | AT phanghuiqun popx2phosphataseregulatesthekif3kinesinmotorcomplex AT hoonjingling popx2phosphataseregulatesthekif3kinesinmotorcomplex AT laisoakkuan popx2phosphataseregulatesthekif3kinesinmotorcomplex AT zengyukai popx2phosphataseregulatesthekif3kinesinmotorcomplex AT chiamkenghwee popx2phosphataseregulatesthekif3kinesinmotorcomplex AT lihoiyeung popx2phosphataseregulatesthekif3kinesinmotorcomplex AT kohchenggee popx2phosphataseregulatesthekif3kinesinmotorcomplex |