Metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases
Carotenoid production from three strains of Rhodosporidium toruloides grown on glycerol was studied. A time-dependent metabolomics approach was used to understand its metabolism on glycerol and mechanism for carotenoid production in three strains during different growth phases (1, 4, 7, and 12 days)...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Online Access: | https://hdl.handle.net/10356/106843 http://hdl.handle.net/10220/25205 |
| _version_ | 1826114421712224256 |
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| author | Lee, Jaslyn Jie Lin Chen, Liwei Shi, Jiahua Trzcinski, Antoine Chen, Wei Ning |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Lee, Jaslyn Jie Lin Chen, Liwei Shi, Jiahua Trzcinski, Antoine Chen, Wei Ning |
| author_sort | Lee, Jaslyn Jie Lin |
| collection | NTU |
| description | Carotenoid production from three strains of Rhodosporidium toruloides grown on glycerol was studied. A time-dependent metabolomics approach was used to understand its metabolism on glycerol and mechanism for carotenoid production in three strains during different growth phases (1, 4, 7, and 12 days). Strain CBS 5490 was the highest carotenoid producer (28.5 mg/L) and had a unique metabolic profile. In this strain, metabolites belonging to the TCA cycle and amino acids were produced in lower amounts, as compared to the other strains. On the other hand, it produced the highest amounts of carotenoid and fatty acid metabolites. This indicated that the lower production of the TCA cycle and amino acid metabolites promoted energy and metabolic flux toward the carotenoid and fatty acid synthesis metabolic pathways. This study shows that metabolomic profiling is a useful tool to gain insight into the metabolic pathways in the cell and to shed light on the different molecular mechanisms between strains. |
| first_indexed | 2024-10-01T03:38:54Z |
| format | Journal Article |
| id | ntu-10356/106843 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:38:54Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1068432020-09-26T21:58:45Z Metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases Lee, Jaslyn Jie Lin Chen, Liwei Shi, Jiahua Trzcinski, Antoine Chen, Wei Ning School of Chemical and Biomedical Engineering School of Civil and Environmental Engineering Nanyang Environment and Water Research Institute DRNTU::Engineering::Environmental engineering Carotenoid production from three strains of Rhodosporidium toruloides grown on glycerol was studied. A time-dependent metabolomics approach was used to understand its metabolism on glycerol and mechanism for carotenoid production in three strains during different growth phases (1, 4, 7, and 12 days). Strain CBS 5490 was the highest carotenoid producer (28.5 mg/L) and had a unique metabolic profile. In this strain, metabolites belonging to the TCA cycle and amino acids were produced in lower amounts, as compared to the other strains. On the other hand, it produced the highest amounts of carotenoid and fatty acid metabolites. This indicated that the lower production of the TCA cycle and amino acid metabolites promoted energy and metabolic flux toward the carotenoid and fatty acid synthesis metabolic pathways. This study shows that metabolomic profiling is a useful tool to gain insight into the metabolic pathways in the cell and to shed light on the different molecular mechanisms between strains. Accepted version 2015-03-10T00:54:16Z 2019-12-06T22:19:31Z 2015-03-10T00:54:16Z 2019-12-06T22:19:31Z 2014 2014 Journal Article Lee, J. J. L., Chen, L., Shi, J., Trzcinski, A., & Chen, W.-N. (2014). Metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases. Journal of agricultural and food chemistry, 62(41), 10203-10209. https://hdl.handle.net/10356/106843 http://hdl.handle.net/10220/25205 10.1021/jf502987q en Journal of agricultural and food chemistry © 2014 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Agricultural and Food Chemistry, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/jf502987q]. 11 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Environmental engineering Lee, Jaslyn Jie Lin Chen, Liwei Shi, Jiahua Trzcinski, Antoine Chen, Wei Ning Metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases |
| title | Metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases |
| title_full | Metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases |
| title_fullStr | Metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases |
| title_full_unstemmed | Metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases |
| title_short | Metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases |
| title_sort | metabolomic profiling of rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases |
| topic | DRNTU::Engineering::Environmental engineering |
| url | https://hdl.handle.net/10356/106843 http://hdl.handle.net/10220/25205 |
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