“Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules
A “turn-on” thiol-responsive fluorescence probe was synthesized and integrated into polymeric nanoparticles for sensing intracellular thiols. There is a photo-induced electron transfer process in the off state of the probe, and this process is terminated upon the reaction with thiol compounds. Confi...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Online Access: | https://hdl.handle.net/10356/106905 http://hdl.handle.net/10220/25237 |
| _version_ | 1826123644123742208 |
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| author | Li, Menghuan Li, Peizhou Zhang, Quan Selvan, Subramanian Tamil Zhao, Yanli Ang, Chung Yen Tan, Si Yu Lu, Yunpeng Bai, Linyi |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Li, Menghuan Li, Peizhou Zhang, Quan Selvan, Subramanian Tamil Zhao, Yanli Ang, Chung Yen Tan, Si Yu Lu, Yunpeng Bai, Linyi |
| author_sort | Li, Menghuan |
| collection | NTU |
| description | A “turn-on” thiol-responsive fluorescence probe was synthesized and integrated into polymeric nanoparticles for sensing intracellular thiols. There is a photo-induced electron transfer process in the off state of the probe, and this process is terminated upon the reaction with thiol compounds. Configuration interaction singles (CIS) calculation was performed to confirm the mechanism of this process. A series of sensing studies were carried out, showing that the probe-integrated nanoparticles were highly selective towards biological thiol compounds over non-thiolated amino acids. Kinetic studies were also performed to investigate the relative reaction rate between the probe and the thiolated amino acids. Subsequently, the Gibbs free energy of the reactions was explored by means of the electrochemical method. Finally, the detection system was employed for sensing intracellular thiols in cancer cells, and the sensing selectivity could be further enhanced with the use of a cancer cell-targeting ligand in the nanoparticles. This development paves a path for the sensing and detection of biological thiols, serving as a potential diagnostic tool in the future. |
| first_indexed | 2024-10-01T06:08:01Z |
| format | Journal Article |
| id | ntu-10356/106905 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:08:01Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1069052023-02-28T19:30:27Z “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules Li, Menghuan Li, Peizhou Zhang, Quan Selvan, Subramanian Tamil Zhao, Yanli Ang, Chung Yen Tan, Si Yu Lu, Yunpeng Bai, Linyi School of Materials Science & Engineering School of Physical and Mathematical Sciences DRNTU::Engineering::Nanotechnology A “turn-on” thiol-responsive fluorescence probe was synthesized and integrated into polymeric nanoparticles for sensing intracellular thiols. There is a photo-induced electron transfer process in the off state of the probe, and this process is terminated upon the reaction with thiol compounds. Configuration interaction singles (CIS) calculation was performed to confirm the mechanism of this process. A series of sensing studies were carried out, showing that the probe-integrated nanoparticles were highly selective towards biological thiol compounds over non-thiolated amino acids. Kinetic studies were also performed to investigate the relative reaction rate between the probe and the thiolated amino acids. Subsequently, the Gibbs free energy of the reactions was explored by means of the electrochemical method. Finally, the detection system was employed for sensing intracellular thiols in cancer cells, and the sensing selectivity could be further enhanced with the use of a cancer cell-targeting ligand in the nanoparticles. This development paves a path for the sensing and detection of biological thiols, serving as a potential diagnostic tool in the future. Published version 2015-03-11T07:48:28Z 2019-12-06T22:20:42Z 2015-03-11T07:48:28Z 2019-12-06T22:20:42Z 2014 2014 Journal Article Ang, C. Y., Tan, S. Y., Lu, Y., Bai, L., Li, M., Li, P., et al. (2014). “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules. Scientific reports, 4. 2045-2322 https://hdl.handle.net/10356/106905 http://hdl.handle.net/10220/25237 10.1038/srep07057 25394758 en Scientific reports This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ 8 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Nanotechnology Li, Menghuan Li, Peizhou Zhang, Quan Selvan, Subramanian Tamil Zhao, Yanli Ang, Chung Yen Tan, Si Yu Lu, Yunpeng Bai, Linyi “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules |
| title | “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules |
| title_full | “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules |
| title_fullStr | “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules |
| title_full_unstemmed | “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules |
| title_short | “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules |
| title_sort | turn on fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules |
| topic | DRNTU::Engineering::Nanotechnology |
| url | https://hdl.handle.net/10356/106905 http://hdl.handle.net/10220/25237 |
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