Chromatin compaction under mixed salt conditions : opposite effects of sodium and potassium ions on nucleosome array folding
It is well known that chromatin structure is highly sensitive to the ionic environment. However, the combined effects of a physiologically relevant mixed ionic environment of K+, Mg2+ and Na+, which are the main cations of the cell cytoplasm, has not been systematically investigated. We studied fold...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/106981 http://hdl.handle.net/10220/25236 |
| _version_ | 1811686934748069888 |
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| author | Allahverdi, Abdollah Chen, Qinming Korolev, Nikolay Nordenskiöld, Lars |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Allahverdi, Abdollah Chen, Qinming Korolev, Nikolay Nordenskiöld, Lars |
| author_sort | Allahverdi, Abdollah |
| collection | NTU |
| description | It is well known that chromatin structure is highly sensitive to the ionic environment. However, the combined effects of a physiologically relevant mixed ionic environment of K+, Mg2+ and Na+, which are the main cations of the cell cytoplasm, has not been systematically investigated. We studied folding and self-association (aggregation) of recombinant 12-mer nucleosome arrays with 177 bp DNA repeat length in solutions of mixtures of K+ and Mg2+ or Na+ and Mg2+. In the presence of Mg2+, the addition of sodium ions promotes folding of array into 30-nm fibres, whereas in mixtures of K+ and Mg2+, potassium ions abrogate folding. We found that self-association of nucleosome arrays in mixed salt solutions is synergistically promoted by Mg2+ and monovalent ions, with sodium being slightly more efficient than potassium in amplifying the self-association. The results highlight the importance of a mixed ionic environment for the compaction of chromatin under physiological conditions and demonstrate the complicated nature of the various factors that determine and regulate chromatin compaction in vivo. |
| first_indexed | 2024-10-01T05:08:19Z |
| format | Journal Article |
| id | ntu-10356/106981 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:08:19Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1069812023-02-28T17:03:42Z Chromatin compaction under mixed salt conditions : opposite effects of sodium and potassium ions on nucleosome array folding Allahverdi, Abdollah Chen, Qinming Korolev, Nikolay Nordenskiöld, Lars School of Biological Sciences DRNTU::Science::Biological sciences It is well known that chromatin structure is highly sensitive to the ionic environment. However, the combined effects of a physiologically relevant mixed ionic environment of K+, Mg2+ and Na+, which are the main cations of the cell cytoplasm, has not been systematically investigated. We studied folding and self-association (aggregation) of recombinant 12-mer nucleosome arrays with 177 bp DNA repeat length in solutions of mixtures of K+ and Mg2+ or Na+ and Mg2+. In the presence of Mg2+, the addition of sodium ions promotes folding of array into 30-nm fibres, whereas in mixtures of K+ and Mg2+, potassium ions abrogate folding. We found that self-association of nucleosome arrays in mixed salt solutions is synergistically promoted by Mg2+ and monovalent ions, with sodium being slightly more efficient than potassium in amplifying the self-association. The results highlight the importance of a mixed ionic environment for the compaction of chromatin under physiological conditions and demonstrate the complicated nature of the various factors that determine and regulate chromatin compaction in vivo. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version 2015-03-11T07:11:34Z 2019-12-06T22:22:22Z 2015-03-11T07:11:34Z 2019-12-06T22:22:22Z 2015 2015 Journal Article Allahverdi, A., Chen, Q., Korolev, N., & Nordenskiöld, L. (2015). Chromatin compaction under mixed salt conditions : opposite effects of sodium and potassium ions on nucleosome array folding. Scientific reports, 5. 2045-2322 https://hdl.handle.net/10356/106981 http://hdl.handle.net/10220/25236 10.1038/srep08512 25688036 en Scientific reports This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ 7 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Allahverdi, Abdollah Chen, Qinming Korolev, Nikolay Nordenskiöld, Lars Chromatin compaction under mixed salt conditions : opposite effects of sodium and potassium ions on nucleosome array folding |
| title | Chromatin compaction under mixed salt conditions : opposite effects of sodium and potassium ions on nucleosome array folding |
| title_full | Chromatin compaction under mixed salt conditions : opposite effects of sodium and potassium ions on nucleosome array folding |
| title_fullStr | Chromatin compaction under mixed salt conditions : opposite effects of sodium and potassium ions on nucleosome array folding |
| title_full_unstemmed | Chromatin compaction under mixed salt conditions : opposite effects of sodium and potassium ions on nucleosome array folding |
| title_short | Chromatin compaction under mixed salt conditions : opposite effects of sodium and potassium ions on nucleosome array folding |
| title_sort | chromatin compaction under mixed salt conditions opposite effects of sodium and potassium ions on nucleosome array folding |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/106981 http://hdl.handle.net/10220/25236 |
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