Structural features of NS3 of Dengue virus serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin
Dengue virus (DENV), which has four serotypes (DENV‐1 to DENV‐4), is the causative agent of the viral infection dengue. DENV nonstructural protein 3 (NS3) comprises a serine protease domain and an RNA helicase domain which has nucleotide triphosphatase activities that are essential for RNA replicati...
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| Format: | Journal Article |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/10356/106990 http://hdl.handle.net/10220/49385 |
| _version_ | 1811696437687222272 |
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| author | Manimekalai, Malathy Sony Subramanian Grüber, Ardina Joon, Shin Matsui, Tsutomu Weiss, Thomas M. Grüber, Gerhard Pan, Ankita Saw, Wuan Geok |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Manimekalai, Malathy Sony Subramanian Grüber, Ardina Joon, Shin Matsui, Tsutomu Weiss, Thomas M. Grüber, Gerhard Pan, Ankita Saw, Wuan Geok |
| author_sort | Manimekalai, Malathy Sony Subramanian |
| collection | NTU |
| description | Dengue virus (DENV), which has four serotypes (DENV‐1 to DENV‐4), is the causative agent of the viral infection dengue. DENV nonstructural protein 3 (NS3) comprises a serine protease domain and an RNA helicase domain which has nucleotide triphosphatase activities that are essential for RNA replication and viral assembly. Here, solution X‐ray scattering was used to provide insight into the overall structure and flexibility of the entire NS3 and its recombinant helicase and protease domains for Dengue virus serotypes 2 and 4 in solution. The DENV‐2 and DENV‐4 NS3 forms are elongated and flexible in solution. The importance of the linker residues in flexibility and domain–domain arrangement was shown by the compactness of the individual protease and helicase domains. Swapping of the 174PPAVP179 linker stretch of the related Hepatitis C virus (HCV) NS3 into DENV‐2 NS3 did not alter the elongated shape of the engineered mutant. Conformational alterations owing to RNA binding are described in the protease domain, which undergoes substantial conformational alterations that are required for the optimal catalysis of bound RNA. Finally, the effects of ATPase inhibitors on the enzymatically active DENV‐2 and DENV‐4 NS3 and the individual helicases are presented, and insight into the allosteric effect of the inhibitor quercetin is provided. |
| first_indexed | 2024-10-01T07:39:21Z |
| format | Journal Article |
| id | ntu-10356/106990 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:39:21Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1069902023-02-28T17:04:13Z Structural features of NS3 of Dengue virus serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin Manimekalai, Malathy Sony Subramanian Grüber, Ardina Joon, Shin Matsui, Tsutomu Weiss, Thomas M. Grüber, Gerhard Pan, Ankita Saw, Wuan Geok School of Biological Sciences Dengue Flavivirus DRNTU::Science::Biological sciences Dengue virus (DENV), which has four serotypes (DENV‐1 to DENV‐4), is the causative agent of the viral infection dengue. DENV nonstructural protein 3 (NS3) comprises a serine protease domain and an RNA helicase domain which has nucleotide triphosphatase activities that are essential for RNA replication and viral assembly. Here, solution X‐ray scattering was used to provide insight into the overall structure and flexibility of the entire NS3 and its recombinant helicase and protease domains for Dengue virus serotypes 2 and 4 in solution. The DENV‐2 and DENV‐4 NS3 forms are elongated and flexible in solution. The importance of the linker residues in flexibility and domain–domain arrangement was shown by the compactness of the individual protease and helicase domains. Swapping of the 174PPAVP179 linker stretch of the related Hepatitis C virus (HCV) NS3 into DENV‐2 NS3 did not alter the elongated shape of the engineered mutant. Conformational alterations owing to RNA binding are described in the protease domain, which undergoes substantial conformational alterations that are required for the optimal catalysis of bound RNA. Finally, the effects of ATPase inhibitors on the enzymatically active DENV‐2 and DENV‐4 NS3 and the individual helicases are presented, and insight into the allosteric effect of the inhibitor quercetin is provided. MOE (Min. of Education, S’pore) Published version 2019-07-17T01:05:50Z 2019-12-06T22:22:36Z 2019-07-17T01:05:50Z 2019-12-06T22:22:36Z 2017 Journal Article Pan, A., Saw, W. G., Manimekalai, M. S. S., Grüber, A., Joon, S., Matsui, T., . . . Grüber, G. (2017). Structural features of NS3 of Dengue virus serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin. Acta Crystallographica Section D Structural Biology, 73(5), 402-419. doi:10.1107/S2059798317003849 https://hdl.handle.net/10356/106990 http://hdl.handle.net/10220/49385 10.1107/S2059798317003849 en Acta Crystallographica Section D Structural Biology © 2017 International Union of Crystallography. All rights reserved. This paper was published in Acta Crystallographica Section D Structural Biology and is made available with permission of International Union of Crystallography. 19 p. application/pdf |
| spellingShingle | Dengue Flavivirus DRNTU::Science::Biological sciences Manimekalai, Malathy Sony Subramanian Grüber, Ardina Joon, Shin Matsui, Tsutomu Weiss, Thomas M. Grüber, Gerhard Pan, Ankita Saw, Wuan Geok Structural features of NS3 of Dengue virus serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin |
| title | Structural features of NS3 of
Dengue virus
serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin |
| title_full | Structural features of NS3 of
Dengue virus
serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin |
| title_fullStr | Structural features of NS3 of
Dengue virus
serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin |
| title_full_unstemmed | Structural features of NS3 of
Dengue virus
serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin |
| title_short | Structural features of NS3 of
Dengue virus
serotypes 2 and 4 in solution and insight into RNA binding and the inhibitory role of quercetin |
| title_sort | structural features of ns3 of dengue virus serotypes 2 and 4 in solution and insight into rna binding and the inhibitory role of quercetin |
| topic | Dengue Flavivirus DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/106990 http://hdl.handle.net/10220/49385 |
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