Dielectrophoresis of spermatozoa in viscoelastic medium
Knowledge of spermatozoa motility is important in selecting suitable spermatozoa for assisted reproduction procedures. By considering the internal sliding force within an active filament, its shape in a viscoelastic Oldroyd-B fluid subjected to nonuniform electric field is presented. The resulting v...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/107078 http://hdl.handle.net/10220/25376 http://dx.doi.org/10.1002/elps.201400326 |
| _version_ | 1811681269103198208 |
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| author | Koh, James Boon Yong Marcos |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Koh, James Boon Yong Marcos |
| author_sort | Koh, James Boon Yong |
| collection | NTU |
| description | Knowledge of spermatozoa motility is important in selecting suitable spermatozoa for assisted reproduction procedures. By considering the internal sliding force within an active filament, its shape in a viscoelastic Oldroyd-B fluid subjected to nonuniform electric field is presented. The resulting velocity is a function of the beating pattern and DEP force, which is dependent on the spermatozoon's morphology as well as the gradient of the mean square electric field. Finally, the velocities of the X- and Y-spermatozoa are compared under various conditions of nonuniform electric field and viscoelasticity of the medium. The presence of DEP force alters their velocities to different extents, giving an 84% level of confidence for selecting spermatozoon that contains the chromosome leading to a female. Therefore, a nonuniform electric field can be used not only to sort and select spermatozoa of desired morphology, but also for gender selection. Moreover, we found that sorting in a viscoelastic fluid medium is more effective as the effect of DEP on the spermatozoa velocity is enhanced by an order of magnitude. |
| first_indexed | 2024-10-01T03:38:15Z |
| format | Journal Article |
| id | ntu-10356/107078 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:38:15Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1070782019-12-06T22:24:15Z Dielectrophoresis of spermatozoa in viscoelastic medium Koh, James Boon Yong Marcos School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering::Fluid mechanics Knowledge of spermatozoa motility is important in selecting suitable spermatozoa for assisted reproduction procedures. By considering the internal sliding force within an active filament, its shape in a viscoelastic Oldroyd-B fluid subjected to nonuniform electric field is presented. The resulting velocity is a function of the beating pattern and DEP force, which is dependent on the spermatozoon's morphology as well as the gradient of the mean square electric field. Finally, the velocities of the X- and Y-spermatozoa are compared under various conditions of nonuniform electric field and viscoelasticity of the medium. The presence of DEP force alters their velocities to different extents, giving an 84% level of confidence for selecting spermatozoon that contains the chromosome leading to a female. Therefore, a nonuniform electric field can be used not only to sort and select spermatozoa of desired morphology, but also for gender selection. Moreover, we found that sorting in a viscoelastic fluid medium is more effective as the effect of DEP on the spermatozoa velocity is enhanced by an order of magnitude. 2015-04-13T02:38:31Z 2019-12-06T22:24:15Z 2015-04-13T02:38:31Z 2019-12-06T22:24:15Z 2015 2015 Journal Article Koh, J. B. Y., & Marcos. (2015). Dielectrophoresis of spermatozoa in viscoelastic medium. Electrophoresis, 36(13), 1514-1521. 0173-0835 https://hdl.handle.net/10356/107078 http://hdl.handle.net/10220/25376 http://dx.doi.org/10.1002/elps.201400326 en Electrophoresis © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Engineering::Mechanical engineering::Fluid mechanics Koh, James Boon Yong Marcos Dielectrophoresis of spermatozoa in viscoelastic medium |
| title | Dielectrophoresis of spermatozoa in viscoelastic medium |
| title_full | Dielectrophoresis of spermatozoa in viscoelastic medium |
| title_fullStr | Dielectrophoresis of spermatozoa in viscoelastic medium |
| title_full_unstemmed | Dielectrophoresis of spermatozoa in viscoelastic medium |
| title_short | Dielectrophoresis of spermatozoa in viscoelastic medium |
| title_sort | dielectrophoresis of spermatozoa in viscoelastic medium |
| topic | DRNTU::Engineering::Mechanical engineering::Fluid mechanics |
| url | https://hdl.handle.net/10356/107078 http://hdl.handle.net/10220/25376 http://dx.doi.org/10.1002/elps.201400326 |
| work_keys_str_mv | AT kohjamesboonyong dielectrophoresisofspermatozoainviscoelasticmedium AT marcos dielectrophoresisofspermatozoainviscoelasticmedium |