Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites
This study examined the relationship between the abundance of bacterial denitrifiers in groundwater at four sites, differing with respect to overlaying land management and peizometer depth. Groundwater was sourced from 36 multilevel piezometers, which were installed to target different groundwater z...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/107234 http://hdl.handle.net/10220/17095 http://dx.doi.org/10.1007/s11356-013-1729-3 |
| _version_ | 1811694686617731072 |
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| author | Barrett, Maria Lee, Changsoo Bhreathnach, Niamh Collins, Gavin O’Flaherty, Vincent Jahangir, Mohammad M. R. Smith, Cindy J. Richards, Karl G. |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Barrett, Maria Lee, Changsoo Bhreathnach, Niamh Collins, Gavin O’Flaherty, Vincent Jahangir, Mohammad M. R. Smith, Cindy J. Richards, Karl G. |
| author_sort | Barrett, Maria |
| collection | NTU |
| description | This study examined the relationship between the abundance of bacterial denitrifiers in groundwater at four sites, differing with respect to overlaying land management and peizometer depth. Groundwater was sourced from 36 multilevel piezometers, which were installed to target different groundwater zones: (1) subsoil, (2) subsoil to bedrock interface, and (3) bedrock. The gene copy concentrations (GCCs), as gene copies per liter, for bacterial 16S rRNA genes and the denitrifying functional genes, nirK, nirS, and nosZ, were determined using quantitative polymerase chain reaction assays. The results were related to gaseous nitrogen emissions and to the physicochemical properties of the four sites. Overall, nirK and nirS abundance appeared to show no significant correlation to N2O production (P = 0.9989; P = 0.3188); and no significant correlation was observed between nosZ and excess N2 concentrations (P = 0.0793). In the majority of piezometers investigated, the variation of nirK and nirS gene copy concentrations was considered significant (P < 0.0001). Dissolved organic carbon (DOC) decreased with aquifer depth and ranged from 1.0–4.0 mg l−1, 0.9–2.4 mg l−1, and 0.8–2.4 mg l−1 within piezometers located in the subsoil, subsoil/bedrock interface, and bedrock depths, respectively. The availability of increasing DOC and the depth of the water table were positively correlated with increasing nir and nosZ GCCs (P = 0.0012). A significant temporal correlation was noted between nirS and piezometer depth (P < 0.001). Interestingly, the nirK, nirS, and nosZ GCCs varied between piezometer depths within specific sites, while GCCs remained relatively constant from site to site, thus indicating no direct impact of agricultural land management strategies investigated on denitrifier abundance. |
| first_indexed | 2024-10-01T07:11:31Z |
| format | Journal Article |
| id | ntu-10356/107234 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:11:31Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1072342019-12-06T22:27:09Z Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites Barrett, Maria Lee, Changsoo Bhreathnach, Niamh Collins, Gavin O’Flaherty, Vincent Jahangir, Mohammad M. R. Smith, Cindy J. Richards, Karl G. School of Civil and Environmental Engineering DRNTU::Engineering::Environmental engineering::Environmental pollution This study examined the relationship between the abundance of bacterial denitrifiers in groundwater at four sites, differing with respect to overlaying land management and peizometer depth. Groundwater was sourced from 36 multilevel piezometers, which were installed to target different groundwater zones: (1) subsoil, (2) subsoil to bedrock interface, and (3) bedrock. The gene copy concentrations (GCCs), as gene copies per liter, for bacterial 16S rRNA genes and the denitrifying functional genes, nirK, nirS, and nosZ, were determined using quantitative polymerase chain reaction assays. The results were related to gaseous nitrogen emissions and to the physicochemical properties of the four sites. Overall, nirK and nirS abundance appeared to show no significant correlation to N2O production (P = 0.9989; P = 0.3188); and no significant correlation was observed between nosZ and excess N2 concentrations (P = 0.0793). In the majority of piezometers investigated, the variation of nirK and nirS gene copy concentrations was considered significant (P < 0.0001). Dissolved organic carbon (DOC) decreased with aquifer depth and ranged from 1.0–4.0 mg l−1, 0.9–2.4 mg l−1, and 0.8–2.4 mg l−1 within piezometers located in the subsoil, subsoil/bedrock interface, and bedrock depths, respectively. The availability of increasing DOC and the depth of the water table were positively correlated with increasing nir and nosZ GCCs (P = 0.0012). A significant temporal correlation was noted between nirS and piezometer depth (P < 0.001). Interestingly, the nirK, nirS, and nosZ GCCs varied between piezometer depths within specific sites, while GCCs remained relatively constant from site to site, thus indicating no direct impact of agricultural land management strategies investigated on denitrifier abundance. 2013-10-31T01:41:11Z 2019-12-06T22:27:09Z 2013-10-31T01:41:11Z 2019-12-06T22:27:09Z 2013 2013 Journal Article Barrett, M., Jahangir, M. M. R., Lee, C., Smith, C. J., Bhreathnach, N., Collins, G., et al. (2013). Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites. Environmental science and pollution research, 20(9), 6646-6657. 0944-1344 https://hdl.handle.net/10356/107234 http://hdl.handle.net/10220/17095 http://dx.doi.org/10.1007/s11356-013-1729-3 en Environmental science and pollution research |
| spellingShingle | DRNTU::Engineering::Environmental engineering::Environmental pollution Barrett, Maria Lee, Changsoo Bhreathnach, Niamh Collins, Gavin O’Flaherty, Vincent Jahangir, Mohammad M. R. Smith, Cindy J. Richards, Karl G. Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites |
| title | Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites |
| title_full | Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites |
| title_fullStr | Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites |
| title_full_unstemmed | Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites |
| title_short | Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites |
| title_sort | abundance of denitrification genes under different peizometer depths in four irish agricultural groundwater sites |
| topic | DRNTU::Engineering::Environmental engineering::Environmental pollution |
| url | https://hdl.handle.net/10356/107234 http://hdl.handle.net/10220/17095 http://dx.doi.org/10.1007/s11356-013-1729-3 |
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