Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model
One bottleneck in tissue regeneration with hydrogel scaffolds is the limited understanding of the crucial factors for controlling hydrogel's physical microenvironments to regulate cell fate. Here, the effects of permeability and living space of hydrogels on encapsulated cells' behavior wer...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Online Access: | https://hdl.handle.net/10356/107327 http://hdl.handle.net/10220/25541 http://dx.doi.org/10.1002/mabi.201400453 |
| _version_ | 1811685930710335488 |
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| author | Fan, Changjiang Wang, Dong-An |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Fan, Changjiang Wang, Dong-An |
| author_sort | Fan, Changjiang |
| collection | NTU |
| description | One bottleneck in tissue regeneration with hydrogel scaffolds is the limited understanding of the crucial factors for controlling hydrogel's physical microenvironments to regulate cell fate. Here, the effects of permeability and living space of hydrogels on encapsulated cells' behavior were evaluated, respectively. Three model hydrogel-based constructs are fabricated by using photo-crosslinkable hyaluronic acid as precursor and chondrocytes as model cell type. The better permeable hydrogels facilitate better cell viability and rapid proliferation, which lead to increased production of extracellular matrix (ECM), e.g. collagen, glycosaminoglycan. By prolonged culture, nano-sized hydrogel networks inhibit neo-tissue development, and the presence of macro-porous living spaces significantly enhance ECM deposition via forming larger cell clusters and eventually induce formation of scaffold-free neo-tissue islets. The results of this work demonstrate that the manipulation and optimization of hydrogel microenvironments, namely permeability and living space, are crucial to direct cell fate and neo-tissue formation. |
| first_indexed | 2024-10-01T04:52:21Z |
| format | Journal Article |
| id | ntu-10356/107327 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:52:21Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1073272019-12-06T22:28:57Z Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model Fan, Changjiang Wang, Dong-An School of Chemical and Biomedical Engineering DRNTU::Science::Biological sciences One bottleneck in tissue regeneration with hydrogel scaffolds is the limited understanding of the crucial factors for controlling hydrogel's physical microenvironments to regulate cell fate. Here, the effects of permeability and living space of hydrogels on encapsulated cells' behavior were evaluated, respectively. Three model hydrogel-based constructs are fabricated by using photo-crosslinkable hyaluronic acid as precursor and chondrocytes as model cell type. The better permeable hydrogels facilitate better cell viability and rapid proliferation, which lead to increased production of extracellular matrix (ECM), e.g. collagen, glycosaminoglycan. By prolonged culture, nano-sized hydrogel networks inhibit neo-tissue development, and the presence of macro-porous living spaces significantly enhance ECM deposition via forming larger cell clusters and eventually induce formation of scaffold-free neo-tissue islets. The results of this work demonstrate that the manipulation and optimization of hydrogel microenvironments, namely permeability and living space, are crucial to direct cell fate and neo-tissue formation. 2015-05-14T06:53:23Z 2019-12-06T22:28:57Z 2015-05-14T06:53:23Z 2019-12-06T22:28:57Z 2015 2015 Journal Article Fan, C., & Wang, D.-A. (2015). Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model. Macromolecular bioscience, 15(4), 535-545. 1616-5187 https://hdl.handle.net/10356/107327 http://hdl.handle.net/10220/25541 http://dx.doi.org/10.1002/mabi.201400453 en Macromolecular bioscience © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Science::Biological sciences Fan, Changjiang Wang, Dong-An Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model |
| title | Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model |
| title_full | Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model |
| title_fullStr | Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model |
| title_full_unstemmed | Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model |
| title_short | Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model |
| title_sort | effects of permeability and living space on cell fate and neo tissue development in hydrogel based scaffolds a study with cartilaginous model |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/107327 http://hdl.handle.net/10220/25541 http://dx.doi.org/10.1002/mabi.201400453 |
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