A unique Oct4 interface is crucial for reprogramming to pluripotency
Terminally differentiated cells can be reprogrammed to pluripotency by the forced expression of Oct4, Sox2, Klf4 and c-Myc1, 2. However, it remains unknown how this leads to the multitude of epigenetic changes observed during the reprogramming process. Interestingly, Oct4 is the only factor that can...
| Main Authors: | , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/107335 http://hdl.handle.net/10220/18028 http://dx.doi.org/10.1038/ncb2680 |
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| author | Esch, Daniel Pogenberg, Vivian Cojocaru, Vlad vom Bruch, Hermann Vahokoski, Juha Groves, Matthew R. Han, Dong Drexler, Hannes C. A. Araúzo-Bravo, Marcos J. Ng, Calista K. L. Jauch, Ralf Wilmanns, Matthias Schöler, Hans R. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Esch, Daniel Pogenberg, Vivian Cojocaru, Vlad vom Bruch, Hermann Vahokoski, Juha Groves, Matthew R. Han, Dong Drexler, Hannes C. A. Araúzo-Bravo, Marcos J. Ng, Calista K. L. Jauch, Ralf Wilmanns, Matthias Schöler, Hans R. |
| author_sort | Esch, Daniel |
| collection | NTU |
| description | Terminally differentiated cells can be reprogrammed to pluripotency by the forced expression of Oct4, Sox2, Klf4 and c-Myc1, 2. However, it remains unknown how this leads to the multitude of epigenetic changes observed during the reprogramming process. Interestingly, Oct4 is the only factor that cannot be replaced by other members of the same family to induce pluripotency3, 4, 5. To understand the unique role of Oct4 in reprogramming, we determined the structure of its POU domain bound to DNA. We show that the linker between the two DNA-binding domains is structured as an α-helix and exposed to the protein’s surface, in contrast to the unstructured linker of Oct1. Point mutations in this α-helix alter or abolish the reprogramming activity of Oct4, but do not affect its other fundamental properties. On the basis of mass spectrometry studies of the interactome of wild-type and mutant Oct4, we propose that the linker functions as a protein–protein interaction interface and plays a crucial role during reprogramming by recruiting key epigenetic players to Oct4 target genes. Thus, we provide molecular insights to explain how Oct4 contributes to the reprogramming process. |
| first_indexed | 2024-10-01T06:30:08Z |
| format | Journal Article |
| id | ntu-10356/107335 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:30:08Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1073352019-12-06T22:29:03Z A unique Oct4 interface is crucial for reprogramming to pluripotency Esch, Daniel Pogenberg, Vivian Cojocaru, Vlad vom Bruch, Hermann Vahokoski, Juha Groves, Matthew R. Han, Dong Drexler, Hannes C. A. Araúzo-Bravo, Marcos J. Ng, Calista K. L. Jauch, Ralf Wilmanns, Matthias Schöler, Hans R. School of Biological Sciences DRNTU::Science::Biological sciences::Cytology Terminally differentiated cells can be reprogrammed to pluripotency by the forced expression of Oct4, Sox2, Klf4 and c-Myc1, 2. However, it remains unknown how this leads to the multitude of epigenetic changes observed during the reprogramming process. Interestingly, Oct4 is the only factor that cannot be replaced by other members of the same family to induce pluripotency3, 4, 5. To understand the unique role of Oct4 in reprogramming, we determined the structure of its POU domain bound to DNA. We show that the linker between the two DNA-binding domains is structured as an α-helix and exposed to the protein’s surface, in contrast to the unstructured linker of Oct1. Point mutations in this α-helix alter or abolish the reprogramming activity of Oct4, but do not affect its other fundamental properties. On the basis of mass spectrometry studies of the interactome of wild-type and mutant Oct4, we propose that the linker functions as a protein–protein interaction interface and plays a crucial role during reprogramming by recruiting key epigenetic players to Oct4 target genes. Thus, we provide molecular insights to explain how Oct4 contributes to the reprogramming process. 2013-12-04T05:05:47Z 2019-12-06T22:29:03Z 2013-12-04T05:05:47Z 2019-12-06T22:29:03Z 2013 2013 Journal Article Esch, D., Vahokoski, J., Groves, M. R., Pogenberg, V., Cojocaru, V., vom Bruch, H. V., et al. (2013). A unique Oct4 interface is crucial for reprogramming to pluripotency. Nature cell biology, 15(3), 295-301. https://hdl.handle.net/10356/107335 http://hdl.handle.net/10220/18028 http://dx.doi.org/10.1038/ncb2680 en Nature cell biology |
| spellingShingle | DRNTU::Science::Biological sciences::Cytology Esch, Daniel Pogenberg, Vivian Cojocaru, Vlad vom Bruch, Hermann Vahokoski, Juha Groves, Matthew R. Han, Dong Drexler, Hannes C. A. Araúzo-Bravo, Marcos J. Ng, Calista K. L. Jauch, Ralf Wilmanns, Matthias Schöler, Hans R. A unique Oct4 interface is crucial for reprogramming to pluripotency |
| title | A unique Oct4 interface is crucial for reprogramming to pluripotency |
| title_full | A unique Oct4 interface is crucial for reprogramming to pluripotency |
| title_fullStr | A unique Oct4 interface is crucial for reprogramming to pluripotency |
| title_full_unstemmed | A unique Oct4 interface is crucial for reprogramming to pluripotency |
| title_short | A unique Oct4 interface is crucial for reprogramming to pluripotency |
| title_sort | unique oct4 interface is crucial for reprogramming to pluripotency |
| topic | DRNTU::Science::Biological sciences::Cytology |
| url | https://hdl.handle.net/10356/107335 http://hdl.handle.net/10220/18028 http://dx.doi.org/10.1038/ncb2680 |
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