Photocrosslinkable gelatin hydrogel for epidermal tissue engineering
Natural hydrogels are promising scaffolds to engineer epidermis. Currently, natural hydrogels used to support epidermal regeneration are mainly collagen- or gelatin-based, which mimic the natural dermal extracellular matrix but often suffer from insufficient and uncontrollable mechanical and degrada...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/107425 http://hdl.handle.net/10220/25527 |
| _version_ | 1826125137572790272 |
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| author | Zhao, Xin Lang, Qi Yildirimer, Lara Lin, Zhi Yuan Cui, Wenguo Annabi, Nasim Ng, Kee Woei Dokmeci, Mehmet R. Ghaemmaghami, Amir M. Khademhosseini, Ali |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Zhao, Xin Lang, Qi Yildirimer, Lara Lin, Zhi Yuan Cui, Wenguo Annabi, Nasim Ng, Kee Woei Dokmeci, Mehmet R. Ghaemmaghami, Amir M. Khademhosseini, Ali |
| author_sort | Zhao, Xin |
| collection | NTU |
| description | Natural hydrogels are promising scaffolds to engineer epidermis. Currently, natural hydrogels used to support epidermal regeneration are mainly collagen- or gelatin-based, which mimic the natural dermal extracellular matrix but often suffer from insufficient and uncontrollable mechanical and degradation properties. In this study, a photocrosslinkable gelatin (i.e., gelatin methacrylamide (GelMA)) with tunable mechanical, degradation, and biological properties is used to engineer the epidermis for skin tissue engineering applications. The results reveal that the mechanical and degradation properties of the developed hydrogels can be readily modified by varying the hydrogel concentration, with elastic and compressive moduli tuned from a few kPa to a few hundred kPa, and the degradation times varied from a few days to several months. Additionally, hydrogels of all concentrations displayed excellent cell viability (>90%) with increasing cell adhesion and proliferation corresponding to increases in hydrogel concentrations. Furthermore, the hydrogels are found to support keratinocyte growth, differentiation, and stratification into a reconstructed multilayered epidermis with adequate barrier functions. The robust and tunable properties of GelMA hydrogels suggest that the keratinocyte laden hydrogels can be used as epidermal substitutes, wound dressings, or substrates to construct various in vitro skin models. |
| first_indexed | 2024-10-01T06:31:46Z |
| format | Journal Article |
| id | ntu-10356/107425 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:31:46Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1074252022-02-16T16:29:46Z Photocrosslinkable gelatin hydrogel for epidermal tissue engineering Zhao, Xin Lang, Qi Yildirimer, Lara Lin, Zhi Yuan Cui, Wenguo Annabi, Nasim Ng, Kee Woei Dokmeci, Mehmet R. Ghaemmaghami, Amir M. Khademhosseini, Ali School of Materials Science & Engineering DRNTU::Science::Medicine::Tissue engineering Natural hydrogels are promising scaffolds to engineer epidermis. Currently, natural hydrogels used to support epidermal regeneration are mainly collagen- or gelatin-based, which mimic the natural dermal extracellular matrix but often suffer from insufficient and uncontrollable mechanical and degradation properties. In this study, a photocrosslinkable gelatin (i.e., gelatin methacrylamide (GelMA)) with tunable mechanical, degradation, and biological properties is used to engineer the epidermis for skin tissue engineering applications. The results reveal that the mechanical and degradation properties of the developed hydrogels can be readily modified by varying the hydrogel concentration, with elastic and compressive moduli tuned from a few kPa to a few hundred kPa, and the degradation times varied from a few days to several months. Additionally, hydrogels of all concentrations displayed excellent cell viability (>90%) with increasing cell adhesion and proliferation corresponding to increases in hydrogel concentrations. Furthermore, the hydrogels are found to support keratinocyte growth, differentiation, and stratification into a reconstructed multilayered epidermis with adequate barrier functions. The robust and tunable properties of GelMA hydrogels suggest that the keratinocyte laden hydrogels can be used as epidermal substitutes, wound dressings, or substrates to construct various in vitro skin models. 2015-05-14T02:53:48Z 2019-12-06T22:30:51Z 2015-05-14T02:53:48Z 2019-12-06T22:30:51Z 2015 2015 Journal Article Zhao, X., Lang, Q., Yildirimer, L., Lin, Z. Y., Cui, W., Annabi, N., et al. (2015). Photocrosslinkable gelatin hydrogel for epidermal tissue engineering. Advanced healthcare materials, 5(1), 108-118. 2192-2640 https://hdl.handle.net/10356/107425 http://hdl.handle.net/10220/25527 10.1002/adhm.201500005 25880725 en Advanced healthcare materials © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Science::Medicine::Tissue engineering Zhao, Xin Lang, Qi Yildirimer, Lara Lin, Zhi Yuan Cui, Wenguo Annabi, Nasim Ng, Kee Woei Dokmeci, Mehmet R. Ghaemmaghami, Amir M. Khademhosseini, Ali Photocrosslinkable gelatin hydrogel for epidermal tissue engineering |
| title | Photocrosslinkable gelatin hydrogel for epidermal tissue engineering |
| title_full | Photocrosslinkable gelatin hydrogel for epidermal tissue engineering |
| title_fullStr | Photocrosslinkable gelatin hydrogel for epidermal tissue engineering |
| title_full_unstemmed | Photocrosslinkable gelatin hydrogel for epidermal tissue engineering |
| title_short | Photocrosslinkable gelatin hydrogel for epidermal tissue engineering |
| title_sort | photocrosslinkable gelatin hydrogel for epidermal tissue engineering |
| topic | DRNTU::Science::Medicine::Tissue engineering |
| url | https://hdl.handle.net/10356/107425 http://hdl.handle.net/10220/25527 |
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