Acetic acid inhibition on methanogens in a two-phase anaerobic process
The inhibitory effect of acetic acid on methanogens in a two-phase anaerobic process was evaluated. The results in this study showed that some methanogens still existed in the acidogenic phase although their dominance in the total microbial community was only 1% compared to 9.6% in the methanogenic...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2013
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| Online Access: | https://hdl.handle.net/10356/107488 http://hdl.handle.net/10220/17906 |
| _version_ | 1824456367109308416 |
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| author | Ng, Wun Jern Guo, Chenghong Zhou, Yan Wang, J. Y. Maspolim, Yogananda Xiao, Keke |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Ng, Wun Jern Guo, Chenghong Zhou, Yan Wang, J. Y. Maspolim, Yogananda Xiao, Keke |
| author_sort | Ng, Wun Jern |
| collection | NTU |
| description | The inhibitory effect of acetic acid on methanogens in a two-phase anaerobic process was evaluated. The results in this study showed that some methanogens still existed in the acidogenic phase although their dominance in the total microbial community was only 1% compared to 9.6% in the methanogenic phase. The inhibition threshold of acetic acid on acidogenic phase methanogens was, however, higher than that on methanogenic phase methanogens. At pH 6.00, acetic acid inhibition on methanogenic phase methanogens was observed when acetic acid concentration was higher than 1619.47 mg HAc/L although there was no obvious inhibition on acidogenic phase methanogens in the range of 1646.47–2781.19 mg HAc/L. There was also no acetic acid inhibition on acidogenic phase methanogens at pH 5.50, 6.00 and 6.50 in the range of 565.29–2781.19 mg HAc/L. However, for methanogenic phase methanogens, the inhibition was obvious and a second order substrate inhibition model, qs = qmS/[Ks + S + (S2/Ki)], could be adapted to describe the inhibition kinetics and mechanism of undissociated acetic acid on methanogenic phase methanogens. The results showed substrate saturation constant Ks, substrate inhibition constant Ki, and maximum specific utilization rate of acetic acid qm, were 1.66 mg unHAc/L, 145.17 mg unHAc/L, and 3.53 mg HAc/L g MLVSS h, respectively. |
| first_indexed | 2025-02-19T03:52:58Z |
| format | Journal Article |
| id | ntu-10356/107488 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:52:58Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1074882020-09-26T22:03:37Z Acetic acid inhibition on methanogens in a two-phase anaerobic process Ng, Wun Jern Guo, Chenghong Zhou, Yan Wang, J. Y. Maspolim, Yogananda Xiao, Keke School of Civil and Environmental Engineering Advanced Environmental Biotechnology Centre (AEBC) Nanyang Environment and Water Research Institute Residues and Resource Reclamation Centre DRNTU::Engineering::Environmental engineering The inhibitory effect of acetic acid on methanogens in a two-phase anaerobic process was evaluated. The results in this study showed that some methanogens still existed in the acidogenic phase although their dominance in the total microbial community was only 1% compared to 9.6% in the methanogenic phase. The inhibition threshold of acetic acid on acidogenic phase methanogens was, however, higher than that on methanogenic phase methanogens. At pH 6.00, acetic acid inhibition on methanogenic phase methanogens was observed when acetic acid concentration was higher than 1619.47 mg HAc/L although there was no obvious inhibition on acidogenic phase methanogens in the range of 1646.47–2781.19 mg HAc/L. There was also no acetic acid inhibition on acidogenic phase methanogens at pH 5.50, 6.00 and 6.50 in the range of 565.29–2781.19 mg HAc/L. However, for methanogenic phase methanogens, the inhibition was obvious and a second order substrate inhibition model, qs = qmS/[Ks + S + (S2/Ki)], could be adapted to describe the inhibition kinetics and mechanism of undissociated acetic acid on methanogenic phase methanogens. The results showed substrate saturation constant Ks, substrate inhibition constant Ki, and maximum specific utilization rate of acetic acid qm, were 1.66 mg unHAc/L, 145.17 mg unHAc/L, and 3.53 mg HAc/L g MLVSS h, respectively. Accepted Version 2013-11-29T04:11:47Z 2019-12-06T22:32:16Z 2013-11-29T04:11:47Z 2019-12-06T22:32:16Z 2013 2013 Journal Article Xiao, K. K., Guo, C., Zhou, Y., Maspolim, Y., Wang, J. Y., & Ng, W. J. (2013). Acetic acid inhibition on methanogens in a two-phase anaerobic process. Biochemical engineering journal, 75,1-7. 1369-703X https://hdl.handle.net/10356/107488 http://hdl.handle.net/10220/17906 10.1016/j.bej.2013.03.011 en Biochemical engineering journal © 2013 Elsevier B.V. This is the author created version of a work that has been peer reviewed and accepted for publication by Biochemical Engineering Journal, Elsevier B.V. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bej.2013.03.011]. application/pdf |
| spellingShingle | DRNTU::Engineering::Environmental engineering Ng, Wun Jern Guo, Chenghong Zhou, Yan Wang, J. Y. Maspolim, Yogananda Xiao, Keke Acetic acid inhibition on methanogens in a two-phase anaerobic process |
| title | Acetic acid inhibition on methanogens in a two-phase anaerobic process |
| title_full | Acetic acid inhibition on methanogens in a two-phase anaerobic process |
| title_fullStr | Acetic acid inhibition on methanogens in a two-phase anaerobic process |
| title_full_unstemmed | Acetic acid inhibition on methanogens in a two-phase anaerobic process |
| title_short | Acetic acid inhibition on methanogens in a two-phase anaerobic process |
| title_sort | acetic acid inhibition on methanogens in a two phase anaerobic process |
| topic | DRNTU::Engineering::Environmental engineering |
| url | https://hdl.handle.net/10356/107488 http://hdl.handle.net/10220/17906 |
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