Fiber optic probe for region of interest (ROI) selective time averaged multi-fluorescence imaging
Time averaged imaging is one of the widely used methods to achieve improved image quality, used in different types of microscopic methods. Time averaged imaging refers to adjusting the exposure time of the imaging system to obtain optimal images. In state of the art microscopes, the region of intere...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/10356/107504 http://hdl.handle.net/10220/49687 |
| _version_ | 1826120926388813824 |
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| author | Shinde, Anant Perinchery, Sandeep Menon Murukeshan, Vadakke Matham |
| author2 | Asundi, Anand K. |
| author_facet | Asundi, Anand K. Shinde, Anant Perinchery, Sandeep Menon Murukeshan, Vadakke Matham |
| author_sort | Shinde, Anant |
| collection | NTU |
| description | Time averaged imaging is one of the widely used methods to achieve improved image quality, used in different types of microscopic methods. Time averaged imaging refers to adjusting the exposure time of the imaging system to obtain optimal images. In state of the art microscopes, the region of interest (ROI) of illumination beam for time averaged imaging can be selected to be of regular shapes such as circle or rectangle. This forces smallest possible ROI to be larger than the actual sample’s ROI which can be of a specific shape with complex contours. In this context, we present a flexible fiber bundle based illumination probe capable of illuminating samples of irregular shapes for time averaged imaging. Further, this probe is capable of multi-wavelength illumination, hence can be used for multi-fluorescence imaging. The fiber probe with features such as region selective and multi- wavelength illumination allows it to be used for optimal imaging of multi-fluorescence sample. |
| first_indexed | 2024-10-01T05:24:25Z |
| format | Journal Article |
| id | ntu-10356/107504 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:24:25Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1075042023-03-04T17:22:05Z Fiber optic probe for region of interest (ROI) selective time averaged multi-fluorescence imaging Shinde, Anant Perinchery, Sandeep Menon Murukeshan, Vadakke Matham Asundi, Anand K. School of Mechanical and Aerospace Engineering Fifth International Conference on Optical and Photonics Engineering Center for Optical and Laser Engineering Fiber Probe Engineering::Mechanical engineering Fiber Optic Imaging Bundle Time averaged imaging is one of the widely used methods to achieve improved image quality, used in different types of microscopic methods. Time averaged imaging refers to adjusting the exposure time of the imaging system to obtain optimal images. In state of the art microscopes, the region of interest (ROI) of illumination beam for time averaged imaging can be selected to be of regular shapes such as circle or rectangle. This forces smallest possible ROI to be larger than the actual sample’s ROI which can be of a specific shape with complex contours. In this context, we present a flexible fiber bundle based illumination probe capable of illuminating samples of irregular shapes for time averaged imaging. Further, this probe is capable of multi-wavelength illumination, hence can be used for multi-fluorescence imaging. The fiber probe with features such as region selective and multi- wavelength illumination allows it to be used for optimal imaging of multi-fluorescence sample. MOE (Min. of Education, S’pore) Published version 2019-08-20T03:27:55Z 2019-12-06T22:32:42Z 2019-08-20T03:27:55Z 2019-12-06T22:32:42Z 2017 Journal Article Shinde, A., Perinchery, S. M., & Vadakke Matham, M. (2017). Fiber optic probe for region of interest (ROI) selective time averaged multi-fluorescence imaging. Fifth International Conference on Optical and Photonics Engineering, 10449, 104490I-. doi:10.1117/12.2269565 0277-786X https://hdl.handle.net/10356/107504 http://hdl.handle.net/10220/49687 10.1117/12.2269565 en Proceedings of SPIE © 2017 SPIE. All rights reserved. This paper was published in Proceedings of SPIE - Fifth International Conference on Optical and Photonics Engineering and is made available with permission of SPIE. 6 p. application/pdf |
| spellingShingle | Fiber Probe Engineering::Mechanical engineering Fiber Optic Imaging Bundle Shinde, Anant Perinchery, Sandeep Menon Murukeshan, Vadakke Matham Fiber optic probe for region of interest (ROI) selective time averaged multi-fluorescence imaging |
| title | Fiber optic probe for region of interest (ROI) selective time averaged multi-fluorescence imaging |
| title_full | Fiber optic probe for region of interest (ROI) selective time averaged multi-fluorescence imaging |
| title_fullStr | Fiber optic probe for region of interest (ROI) selective time averaged multi-fluorescence imaging |
| title_full_unstemmed | Fiber optic probe for region of interest (ROI) selective time averaged multi-fluorescence imaging |
| title_short | Fiber optic probe for region of interest (ROI) selective time averaged multi-fluorescence imaging |
| title_sort | fiber optic probe for region of interest roi selective time averaged multi fluorescence imaging |
| topic | Fiber Probe Engineering::Mechanical engineering Fiber Optic Imaging Bundle |
| url | https://hdl.handle.net/10356/107504 http://hdl.handle.net/10220/49687 |
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