A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury
Despite its high morbidity and mortality, contrast‐induced acute kidney injury (CIAKI) remains a diagnostic dilemma because it relies on in vitro detection of insensitive late‐stage blood and urinary biomarkers. We report the synthesis of an activatable duplex reporter (ADR) for real‐time in vivo im...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/10356/136719 |
| _version_ | 1826113054860902400 |
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| author | Huang, Jiaguo Lyu, Yan Li, Jingchao Cheng, Penghui Jiang, Yuyan Pu, Kanyi |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Huang, Jiaguo Lyu, Yan Li, Jingchao Cheng, Penghui Jiang, Yuyan Pu, Kanyi |
| author_sort | Huang, Jiaguo |
| collection | NTU |
| description | Despite its high morbidity and mortality, contrast‐induced acute kidney injury (CIAKI) remains a diagnostic dilemma because it relies on in vitro detection of insensitive late‐stage blood and urinary biomarkers. We report the synthesis of an activatable duplex reporter (ADR) for real‐time in vivo imaging of CIAKI. ADR is equipped with chemiluminescence and near‐infrared fluorescence (NIRF) signaling channels that can be activated by oxidative stress (superoxide anion, O2.−) and lysosomal damage (N‐acetyl‐β‐d‐glucosaminidase, NAG), respectively. By virtue of its high renal clearance efficiency (80 % injected doses after 24 h injection), ADR detects sequential upregulation of O2.− and NAG in the kidneys of living mice prior to a significant decrease in glomerular filtration rate (GFR) and tissue damage in the course of CIAKI. ADR outperforms the typical clinical assays and detects CIAKI at least 8 h (NIRF) and up to 16 h (chemiluminescence) earlier. |
| first_indexed | 2024-10-01T03:16:54Z |
| format | Journal Article |
| id | ntu-10356/136719 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:16:54Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1367192023-12-29T06:46:24Z A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury Huang, Jiaguo Lyu, Yan Li, Jingchao Cheng, Penghui Jiang, Yuyan Pu, Kanyi School of Chemical and Biomedical Engineering Engineering::Chemical engineering Renal-Clearable Duplex Optical Reporter Acute Kidney Injury Despite its high morbidity and mortality, contrast‐induced acute kidney injury (CIAKI) remains a diagnostic dilemma because it relies on in vitro detection of insensitive late‐stage blood and urinary biomarkers. We report the synthesis of an activatable duplex reporter (ADR) for real‐time in vivo imaging of CIAKI. ADR is equipped with chemiluminescence and near‐infrared fluorescence (NIRF) signaling channels that can be activated by oxidative stress (superoxide anion, O2.−) and lysosomal damage (N‐acetyl‐β‐d‐glucosaminidase, NAG), respectively. By virtue of its high renal clearance efficiency (80 % injected doses after 24 h injection), ADR detects sequential upregulation of O2.− and NAG in the kidneys of living mice prior to a significant decrease in glomerular filtration rate (GFR) and tissue damage in the course of CIAKI. ADR outperforms the typical clinical assays and detects CIAKI at least 8 h (NIRF) and up to 16 h (chemiluminescence) earlier. MOE (Min. of Education, S’pore) Accepted version 2020-01-14T01:46:18Z 2020-01-14T01:46:18Z 2019 2019 Journal Article Huang, J., Lyu, Y., Li, J., Cheng, P., Jiang, Y., & Pu, K. (2019). A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury. Angewandte Chemie International Edition, 58(49), 17796-17804. doi:10.1002/anie.201910137 1433-7851 https://hdl.handle.net/10356/136719 10.1002/anie.201910137 49 58 17796 17804 214921 en Angewandte Chemie International Edition This is the peer reviewed version of the following article: Huang, J., Lyu, Y., Li, J., Cheng, P., Jiang, Y., & Pu, K. (2019). A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury. Angewandte Chemie International Edition, 58(49), 17796-17804. doi:10.1002/anie.201910137, which has been published in final form at https://doi.org/10.1002/anie.201910137. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. application/pdf |
| spellingShingle | Engineering::Chemical engineering Renal-Clearable Duplex Optical Reporter Acute Kidney Injury Huang, Jiaguo Lyu, Yan Li, Jingchao Cheng, Penghui Jiang, Yuyan Pu, Kanyi A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury |
| title | A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury |
| title_full | A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury |
| title_fullStr | A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury |
| title_full_unstemmed | A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury |
| title_short | A renal-clearable duplex optical reporter for real-time imaging of contrast-induced acute kidney injury |
| title_sort | renal clearable duplex optical reporter for real time imaging of contrast induced acute kidney injury |
| topic | Engineering::Chemical engineering Renal-Clearable Duplex Optical Reporter Acute Kidney Injury |
| url | https://hdl.handle.net/10356/136719 |
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