Generate vascularized kidney organoids for studying kidney development
Human pluripotent stem cells (hPSCs)-derived organoids recapitulate organ architecture and development processes. Here we employ a hPSCs-derived three-dimensional (3D) kidney organoid platform to study human kidney development in vitro, which cannot be realized otherwise due to ethical consideration...
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| Format: | Thesis-Master by Research |
| Language: | English |
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Nanyang Technological University
2020
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| Online Access: | https://hdl.handle.net/10356/137292 |
| _version_ | 1824455257145475072 |
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| author | Zhang, Tian |
| author2 | Liang Zhao-Xun |
| author_facet | Liang Zhao-Xun Zhang, Tian |
| author_sort | Zhang, Tian |
| collection | NTU |
| description | Human pluripotent stem cells (hPSCs)-derived organoids recapitulate organ architecture and development processes. Here we employ a hPSCs-derived three-dimensional (3D) kidney organoid platform to study human kidney development in vitro, which cannot be realized otherwise due to ethical considerations. Using this platform, we demonstrate that a subpopulation of nephron progenitor cells (NPCs) display similar property of vascular progenitors, which eventually differentiate to renal endothelial cells in both in vitro differentiation and in vivo development. Based on this platform, we further develop an approach to modulate the differentiation of nephron compartments via manipulating metabolic pathway. Overall, hPSCs-derived kidney organoids offer new avenues for studying renal lineage specification, as well as for disease modeling in an organ-specific manner. |
| first_indexed | 2025-02-19T03:35:20Z |
| format | Thesis-Master by Research |
| id | ntu-10356/137292 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:35:20Z |
| publishDate | 2020 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1372922023-02-28T18:49:55Z Generate vascularized kidney organoids for studying kidney development Zhang, Tian Liang Zhao-Xun Xia Yun School of Biological Sciences ZXLiang@ntu.edu.sg Science::Biological sciences Human pluripotent stem cells (hPSCs)-derived organoids recapitulate organ architecture and development processes. Here we employ a hPSCs-derived three-dimensional (3D) kidney organoid platform to study human kidney development in vitro, which cannot be realized otherwise due to ethical considerations. Using this platform, we demonstrate that a subpopulation of nephron progenitor cells (NPCs) display similar property of vascular progenitors, which eventually differentiate to renal endothelial cells in both in vitro differentiation and in vivo development. Based on this platform, we further develop an approach to modulate the differentiation of nephron compartments via manipulating metabolic pathway. Overall, hPSCs-derived kidney organoids offer new avenues for studying renal lineage specification, as well as for disease modeling in an organ-specific manner. Master of Science 2020-03-16T07:30:12Z 2020-03-16T07:30:12Z 2020 Thesis-Master by Research Zhang, T. (2020). Generate vascularized kidney organoids for studying kidney development. Master's thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/137292 10.32657/10356/137292 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Zhang, Tian Generate vascularized kidney organoids for studying kidney development |
| title | Generate vascularized kidney organoids for studying kidney development |
| title_full | Generate vascularized kidney organoids for studying kidney development |
| title_fullStr | Generate vascularized kidney organoids for studying kidney development |
| title_full_unstemmed | Generate vascularized kidney organoids for studying kidney development |
| title_short | Generate vascularized kidney organoids for studying kidney development |
| title_sort | generate vascularized kidney organoids for studying kidney development |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/137292 |
| work_keys_str_mv | AT zhangtian generatevascularizedkidneyorganoidsforstudyingkidneydevelopment |