Proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade
Primary open angle glaucoma (POAG) is a complex disease and a leading cause of irreversible blindness, and its underlying pathophysiology remains poorly understood. Proteomic characterization of the protein composition of aqueous humor (AH) may identify prognostic candidate proteins involved in path...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Online Access: | https://hdl.handle.net/10356/137389 |
| _version_ | 1811688487912472576 |
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| author | Adav, Sunil Shankar Wei, Jin Yap, Terence Ang, Bryan C. H. Yip, Leonard W. L. Sze, Siu Kwan |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Adav, Sunil Shankar Wei, Jin Yap, Terence Ang, Bryan C. H. Yip, Leonard W. L. Sze, Siu Kwan |
| author_sort | Adav, Sunil Shankar |
| collection | NTU |
| description | Primary open angle glaucoma (POAG) is a complex disease and a leading cause of irreversible blindness, and its underlying pathophysiology remains poorly understood. Proteomic characterization of the protein composition of aqueous humor (AH) may identify prognostic candidate proteins involved in pathogenesis and progression of the disease. To delineate the possible mechanisms that lead to POAG, this study adopted state-of-art mass spectrometric technique and analyzed AH of POAG and their respective controls. In total, more than 1000 proteins were identified with false discovery rate of less than 1%. Numerous proteins of complement cascade, immunoglobulin, neuronal and amyloidogenic proteins, which were part of processes like acute-phase and inflammatory response, humoral immune and acute inflammatory response, regulation of complement activation and protein processing were identified. Proteins of complement system underwent significant changes, which correlate to pathogenic events characterizing POAG, including altered complement cascade, astrocyte activation, neural degeneration, and apoptosis. Further, protein modification such as deamidation of complement subcomponent was noted, particularly in POAG. Proteomic analysis of AH allows a better understanding of the mechanism involved in the pathogenesis of POAG. |
| first_indexed | 2024-10-01T05:33:00Z |
| format | Journal Article |
| id | ntu-10356/137389 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:33:00Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1373892023-02-28T17:07:07Z Proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade Adav, Sunil Shankar Wei, Jin Yap, Terence Ang, Bryan C. H. Yip, Leonard W. L. Sze, Siu Kwan School of Biological Sciences Science::Biological sciences Aqueous Humor Glaucoma Primary open angle glaucoma (POAG) is a complex disease and a leading cause of irreversible blindness, and its underlying pathophysiology remains poorly understood. Proteomic characterization of the protein composition of aqueous humor (AH) may identify prognostic candidate proteins involved in pathogenesis and progression of the disease. To delineate the possible mechanisms that lead to POAG, this study adopted state-of-art mass spectrometric technique and analyzed AH of POAG and their respective controls. In total, more than 1000 proteins were identified with false discovery rate of less than 1%. Numerous proteins of complement cascade, immunoglobulin, neuronal and amyloidogenic proteins, which were part of processes like acute-phase and inflammatory response, humoral immune and acute inflammatory response, regulation of complement activation and protein processing were identified. Proteins of complement system underwent significant changes, which correlate to pathogenic events characterizing POAG, including altered complement cascade, astrocyte activation, neural degeneration, and apoptosis. Further, protein modification such as deamidation of complement subcomponent was noted, particularly in POAG. Proteomic analysis of AH allows a better understanding of the mechanism involved in the pathogenesis of POAG. MOE (Min. of Education, S’pore) NMRC (Natl Medical Research Council, S’pore) Accepted version 2020-03-20T05:03:07Z 2020-03-20T05:03:07Z 2018 Journal Article Adav, S. S., Wei, J., Yap, T., Ang, B. C. H, Yip, L. W. L, & Sze, S. K. (2018). Proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade. Journal of Proteome Research, 17(7), 2499-2510. doi:10.1021/acs.jproteome.8b00244 1535-3893 https://hdl.handle.net/10356/137389 10.1021/acs.jproteome.8b00244 29901396 2-s2.0-85048705561 7 17 2499 2510 en Journal of Proteome Research This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jproteome.8b00244 application/pdf |
| spellingShingle | Science::Biological sciences Aqueous Humor Glaucoma Adav, Sunil Shankar Wei, Jin Yap, Terence Ang, Bryan C. H. Yip, Leonard W. L. Sze, Siu Kwan Proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade |
| title | Proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade |
| title_full | Proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade |
| title_fullStr | Proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade |
| title_full_unstemmed | Proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade |
| title_short | Proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade |
| title_sort | proteomic analysis of aqueous humor from primary open angle glaucoma patients on drug treatment revealed altered complement activation cascade |
| topic | Science::Biological sciences Aqueous Humor Glaucoma |
| url | https://hdl.handle.net/10356/137389 |
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