Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing
Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been shown to possess many advantages such as good biocompatibility, support for cell growth, tunable mechanical properties, photocurable capability, and low material cost. Due to these superior properties, much research has been carri...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/10356/137428 |
| _version_ | 1826118540876316672 |
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| author | Zhou, Miaomiao Lee, Bae Hoon Tan, Yu Jun Tan, Lay Poh |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Zhou, Miaomiao Lee, Bae Hoon Tan, Yu Jun Tan, Lay Poh |
| author_sort | Zhou, Miaomiao |
| collection | NTU |
| description | Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been shown to possess many advantages such as good biocompatibility, support for cell growth, tunable mechanical properties, photocurable capability, and low material cost. Due to these superior properties, much research has been carried out to develop GelMA as a bioink for bioprinting. However, there are still many challenges, and one major challenge is the control of its rheological properties to yield good printability. Herein, this study presents a strategy to control the rheology of GelMA through partial enzymatic crosslinking. Unlike other enzymatic crosslinking strategies where the rheological properties could not be controlled once reaction takes place, we could, to a large extent, keep the rheological properties stable by introducing a deactivation step after obtaining the optimized rheological properties. Ca2+-independent microbial transglutaminase (MTGase) was introduced to partially catalyze covalent bond formation between chains of GelMA. The enzyme was then deactivated to prevent further uncontrolled crosslinking that would render the hydrogel not printable. After printing, a secondary post-printing crosslinking step (photo crosslinking) was then introduced to ensure long-term stability of the printed structure for subsequent cell studies. Biocompatibility studies carried out using cells encapsulated in the printed structure showed excellent cell viability for at least 7 d. This strategy for better control of rheological properties of GelMA could more significantly enhance the usability of this material as bioink for bioprinting of cell-laden structures for soft tissue engineering. |
| first_indexed | 2024-10-01T04:45:07Z |
| format | Journal Article |
| id | ntu-10356/137428 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:45:07Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1374282023-07-14T15:48:49Z Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing Zhou, Miaomiao Lee, Bae Hoon Tan, Yu Jun Tan, Lay Poh School of Materials Science & Engineering School of Mechanical and Aerospace Engineering Singapore Centre for 3D Printing Engineering::Materials Rheological Properties Gelatin Methacryloyl Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been shown to possess many advantages such as good biocompatibility, support for cell growth, tunable mechanical properties, photocurable capability, and low material cost. Due to these superior properties, much research has been carried out to develop GelMA as a bioink for bioprinting. However, there are still many challenges, and one major challenge is the control of its rheological properties to yield good printability. Herein, this study presents a strategy to control the rheology of GelMA through partial enzymatic crosslinking. Unlike other enzymatic crosslinking strategies where the rheological properties could not be controlled once reaction takes place, we could, to a large extent, keep the rheological properties stable by introducing a deactivation step after obtaining the optimized rheological properties. Ca2+-independent microbial transglutaminase (MTGase) was introduced to partially catalyze covalent bond formation between chains of GelMA. The enzyme was then deactivated to prevent further uncontrolled crosslinking that would render the hydrogel not printable. After printing, a secondary post-printing crosslinking step (photo crosslinking) was then introduced to ensure long-term stability of the printed structure for subsequent cell studies. Biocompatibility studies carried out using cells encapsulated in the printed structure showed excellent cell viability for at least 7 d. This strategy for better control of rheological properties of GelMA could more significantly enhance the usability of this material as bioink for bioprinting of cell-laden structures for soft tissue engineering. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Published version 2020-03-25T05:05:48Z 2020-03-25T05:05:48Z 2019 Journal Article Zhou, M., Lee, B. H., Tan, Y. J., & Tan, L. P. (2019). Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing. Biofabrication, 11(2), 025011-. doi:10.1088/1758-5090/ab063f 1758-5082 https://hdl.handle.net/10356/137428 10.1088/1758-5090/ab063f 30743259 2-s2.0-85063937936 2 11 en Biofabrication © 2019 IOP Publishing Ltd. Original content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. application/pdf |
| spellingShingle | Engineering::Materials Rheological Properties Gelatin Methacryloyl Zhou, Miaomiao Lee, Bae Hoon Tan, Yu Jun Tan, Lay Poh Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing |
| title | Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing |
| title_full | Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing |
| title_fullStr | Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing |
| title_full_unstemmed | Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing |
| title_short | Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing |
| title_sort | microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3d printing |
| topic | Engineering::Materials Rheological Properties Gelatin Methacryloyl |
| url | https://hdl.handle.net/10356/137428 |
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