Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”
Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems leads current studies to approximate it simply as an in‐plane stress. To evaluate possible cont...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/137874 |
| _version_ | 1826111085343670272 |
|---|---|
| author | Cai, Pingqiang Li, Zhuyun Keneth, Ela Sachyani Wang, Luying Wan, Changjin Jiang, Ying Hu, Benhui Wu, Yun-Long Wang, Shutao Lim, Chwee Teck Makeyev, Eugene V. Magdassi, Shlomo Chen, Xiaodong |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Cai, Pingqiang Li, Zhuyun Keneth, Ela Sachyani Wang, Luying Wan, Changjin Jiang, Ying Hu, Benhui Wu, Yun-Long Wang, Shutao Lim, Chwee Teck Makeyev, Eugene V. Magdassi, Shlomo Chen, Xiaodong |
| author_sort | Cai, Pingqiang |
| collection | NTU |
| description | Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems leads current studies to approximate it simply as an in‐plane stress. To evaluate possible contribution of force vectors acting in other directions, double epithelium in a 3D‐printed “GeminiChip” containing a sessile and a pendant channel is reconstituted. Intriguingly, the sessile epithelia is prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favors live cell delamination. Transcriptome analyses show upregulation of RhoA, BMP2, and hypoxia‐signaling genes in the pendant epithelium, consistent with the onset of an epithelial–mesenchymal transition program. HepG2 microtumor spheroids also display differential spreading patterns in the sessile and pendant configuration. Using this multilayered GeminiChip, these results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer. |
| first_indexed | 2024-10-01T02:44:51Z |
| format | Journal Article |
| id | ntu-10356/137874 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:44:51Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1378742023-07-14T15:57:22Z Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip” Cai, Pingqiang Li, Zhuyun Keneth, Ela Sachyani Wang, Luying Wan, Changjin Jiang, Ying Hu, Benhui Wu, Yun-Long Wang, Shutao Lim, Chwee Teck Makeyev, Eugene V. Magdassi, Shlomo Chen, Xiaodong School of Materials Science & Engineering Innovative Center for Flexible Devices (iFLEX) Science::Biological sciences 3D Printing Epithelial Homeostasis Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems leads current studies to approximate it simply as an in‐plane stress. To evaluate possible contribution of force vectors acting in other directions, double epithelium in a 3D‐printed “GeminiChip” containing a sessile and a pendant channel is reconstituted. Intriguingly, the sessile epithelia is prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favors live cell delamination. Transcriptome analyses show upregulation of RhoA, BMP2, and hypoxia‐signaling genes in the pendant epithelium, consistent with the onset of an epithelial–mesenchymal transition program. HepG2 microtumor spheroids also display differential spreading patterns in the sessile and pendant configuration. Using this multilayered GeminiChip, these results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer. NRF (Natl Research Foundation, S’pore) Accepted version 2020-04-17T02:21:47Z 2020-04-17T02:21:47Z 2019 Journal Article Cai, P., Li, Z., Keneth, E. S., Wang, L., Wan, C., Jiang, Y., . . . Chen X. (2019). Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”. Advanced Materials, 31(28), 1900514-. doi:10.1002/adma.201900514 0935-9648 https://hdl.handle.net/10356/137874 10.1002/adma.201900514 31081206 2-s2.0-85065706324 28 31 en Advanced materials This is the peer reviewed version of the following article: Cai, P., Li, Z., Keneth, E. S., Wang, L., Wan, C., Jiang, Y., . . . Chen X. (2019). Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”. Advanced Materials, 31(28), 1900514-. doi:10.1002/adma.201900514, which has been published in final form at https://doi.org/10.1002/adma.201900514. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. application/pdf |
| spellingShingle | Science::Biological sciences 3D Printing Epithelial Homeostasis Cai, Pingqiang Li, Zhuyun Keneth, Ela Sachyani Wang, Luying Wan, Changjin Jiang, Ying Hu, Benhui Wu, Yun-Long Wang, Shutao Lim, Chwee Teck Makeyev, Eugene V. Magdassi, Shlomo Chen, Xiaodong Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip” |
| title | Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip” |
| title_full | Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip” |
| title_fullStr | Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip” |
| title_full_unstemmed | Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip” |
| title_short | Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip” |
| title_sort | differential homeostasis of sessile and pendant epithelium reconstituted in a 3d printed geminichip |
| topic | Science::Biological sciences 3D Printing Epithelial Homeostasis |
| url | https://hdl.handle.net/10356/137874 |
| work_keys_str_mv | AT caipingqiang differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT lizhuyun differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT kenethelasachyani differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT wangluying differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT wanchangjin differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT jiangying differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT hubenhui differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT wuyunlong differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT wangshutao differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT limchweeteck differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT makeyeveugenev differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT magdassishlomo differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip AT chenxiaodong differentialhomeostasisofsessileandpendantepitheliumreconstitutedina3dprintedgeminichip |