Development of algae-based living sensors for water quality monitoring

Water pollution is a common problem in developing countries. With nearly 2 billion people not having access to clean water, water quality testing is essential for developing countries to ensure the health of its people. Commercialized water test kits and laboratory testing may not be the most feasib...

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Main Author: Pang, Jerica Shi Hui
Other Authors: Dalton Tay Chor Yong
Format: Final Year Project (FYP)
Language:English
Published: Nanyang Technological University 2020
Subjects:
Online Access:https://hdl.handle.net/10356/138410
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author Pang, Jerica Shi Hui
author2 Dalton Tay Chor Yong
author_facet Dalton Tay Chor Yong
Pang, Jerica Shi Hui
author_sort Pang, Jerica Shi Hui
collection NTU
description Water pollution is a common problem in developing countries. With nearly 2 billion people not having access to clean water, water quality testing is essential for developing countries to ensure the health of its people. Commercialized water test kits and laboratory testing may not be the most feasible and cost-efficient methods for developing countries to use for water testing. Therefore, there is a need to develop an algae-based living sensor for water quality monitoring that is inexpensive and portable. In this project, Phaeodactylum tricornutm (P. tricornutum) is the choice of microorganism for experiments. Phaeodactylum tricornutm is a marine alga that is sensitive to its environmental conditions and can be a good indicator of the health of seawater. In this project, studies of encapsulated P. tricornutm were conducted over a period of 7 days. Results have shown that encapsulated P. tricornutm can stay alive in seawater conditions for up to 14 days. A minimum of 50 beads was required to provide a good correlation between autofluorescence and cell densities of diatoms. Toxicological assays were conducted and it was discovered that encapsulated diatoms can accurately detect a change in the environment within 24 hours.
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spelling ntu-10356/1384102023-03-04T15:45:37Z Development of algae-based living sensors for water quality monitoring Pang, Jerica Shi Hui Dalton Tay Chor Yong School of Materials Science and Engineering cytay@ntu.edu.sg Engineering::Materials Water pollution is a common problem in developing countries. With nearly 2 billion people not having access to clean water, water quality testing is essential for developing countries to ensure the health of its people. Commercialized water test kits and laboratory testing may not be the most feasible and cost-efficient methods for developing countries to use for water testing. Therefore, there is a need to develop an algae-based living sensor for water quality monitoring that is inexpensive and portable. In this project, Phaeodactylum tricornutm (P. tricornutum) is the choice of microorganism for experiments. Phaeodactylum tricornutm is a marine alga that is sensitive to its environmental conditions and can be a good indicator of the health of seawater. In this project, studies of encapsulated P. tricornutm were conducted over a period of 7 days. Results have shown that encapsulated P. tricornutm can stay alive in seawater conditions for up to 14 days. A minimum of 50 beads was required to provide a good correlation between autofluorescence and cell densities of diatoms. Toxicological assays were conducted and it was discovered that encapsulated diatoms can accurately detect a change in the environment within 24 hours. Bachelor of Engineering (Materials Engineering) 2020-05-06T00:26:57Z 2020-05-06T00:26:57Z 2020 Final Year Project (FYP) https://hdl.handle.net/10356/138410 en application/pdf Nanyang Technological University
spellingShingle Engineering::Materials
Pang, Jerica Shi Hui
Development of algae-based living sensors for water quality monitoring
title Development of algae-based living sensors for water quality monitoring
title_full Development of algae-based living sensors for water quality monitoring
title_fullStr Development of algae-based living sensors for water quality monitoring
title_full_unstemmed Development of algae-based living sensors for water quality monitoring
title_short Development of algae-based living sensors for water quality monitoring
title_sort development of algae based living sensors for water quality monitoring
topic Engineering::Materials
url https://hdl.handle.net/10356/138410
work_keys_str_mv AT pangjericashihui developmentofalgaebasedlivingsensorsforwaterqualitymonitoring