Real‐time imaging of dynamic cell reprogramming with nanosensors
Cellular reprogramming, the process by which somatic cells regain pluripotency, is relevant in many disease modeling, therapeutic, and drug discovery applications. Molecular evaluation of reprogramming (e.g., polymerase chain reaction, immunostaining) is typically disruptive, and only provides snaps...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Online Access: | https://hdl.handle.net/10356/139022 |
| _version_ | 1824455092615512064 |
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| author | Wiraja, Christian Yeo, David Chenloong Tham, Khek-Chian Chew, Sharon Wan Ting Lim, Xinhong Xu, Chenjie |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Wiraja, Christian Yeo, David Chenloong Tham, Khek-Chian Chew, Sharon Wan Ting Lim, Xinhong Xu, Chenjie |
| author_sort | Wiraja, Christian |
| collection | NTU |
| description | Cellular reprogramming, the process by which somatic cells regain pluripotency, is relevant in many disease modeling, therapeutic, and drug discovery applications. Molecular evaluation of reprogramming (e.g., polymerase chain reaction, immunostaining) is typically disruptive, and only provides snapshots of phenotypic traits. Gene reporter constructs facilitate live-cell evaluation but is labor intensive and may risk insertional mutagenesis during viral transfection. Herein, the utilization of a non-integrative nanosensor is demonstrated to visualize key reprogramming events in situ within live cells. Principally based on sustained intracellular release of encapsulated molecular probes, nanosensors successfully monitored mesenchymal-epithelial transition, pluripotency acquisition, and transdifferentiation events. Tracking the dynamic expression of four pivotal biomarkers (i.e., THY1, E-CADHERIN, OCT4, and GATA4 mRNA), nanosensor signal showed great agreement with polymerase chain reaction and gene reporter imaging (R2 > 0.9). Overall, such facile, versatile nanosensor enables real-time monitoring of low-frequency reprogramming events, thereby useful for high-throughput assessment, optimization, and biomarker-specific cell enrichment. |
| first_indexed | 2025-02-19T03:32:43Z |
| format | Journal Article |
| id | ntu-10356/139022 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:32:43Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1390222020-05-15T00:56:18Z Real‐time imaging of dynamic cell reprogramming with nanosensors Wiraja, Christian Yeo, David Chenloong Tham, Khek-Chian Chew, Sharon Wan Ting Lim, Xinhong Xu, Chenjie School of Chemical and Biomedical Engineering Interdisciplinary Graduate School (IGS) Lee Kong Chian School of Medicine (LKCMedicine) NTU Institute for Health Technologies NTU-Northwestern Institute for Nanomedicine Engineering::Chemical engineering Cell Reprogramming Molecular Beacons Cellular reprogramming, the process by which somatic cells regain pluripotency, is relevant in many disease modeling, therapeutic, and drug discovery applications. Molecular evaluation of reprogramming (e.g., polymerase chain reaction, immunostaining) is typically disruptive, and only provides snapshots of phenotypic traits. Gene reporter constructs facilitate live-cell evaluation but is labor intensive and may risk insertional mutagenesis during viral transfection. Herein, the utilization of a non-integrative nanosensor is demonstrated to visualize key reprogramming events in situ within live cells. Principally based on sustained intracellular release of encapsulated molecular probes, nanosensors successfully monitored mesenchymal-epithelial transition, pluripotency acquisition, and transdifferentiation events. Tracking the dynamic expression of four pivotal biomarkers (i.e., THY1, E-CADHERIN, OCT4, and GATA4 mRNA), nanosensor signal showed great agreement with polymerase chain reaction and gene reporter imaging (R2 > 0.9). Overall, such facile, versatile nanosensor enables real-time monitoring of low-frequency reprogramming events, thereby useful for high-throughput assessment, optimization, and biomarker-specific cell enrichment. 2020-05-15T00:56:18Z 2020-05-15T00:56:18Z 2018 Journal Article Wiraja, C., Yeo, D. C., Tham, K.-C., Chew, S. W. T., Lim, X., & Xu, C. (2018). Real‐time imaging of dynamic cell reprogramming with nanosensors. Small, 14(17), 1703440-. doi:10.1002/smll.201703440 1613-6810 https://hdl.handle.net/10356/139022 10.1002/smll.201703440 29611333 2-s2.0-85044713563 17 14 en Small © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved. |
| spellingShingle | Engineering::Chemical engineering Cell Reprogramming Molecular Beacons Wiraja, Christian Yeo, David Chenloong Tham, Khek-Chian Chew, Sharon Wan Ting Lim, Xinhong Xu, Chenjie Real‐time imaging of dynamic cell reprogramming with nanosensors |
| title | Real‐time imaging of dynamic cell reprogramming with nanosensors |
| title_full | Real‐time imaging of dynamic cell reprogramming with nanosensors |
| title_fullStr | Real‐time imaging of dynamic cell reprogramming with nanosensors |
| title_full_unstemmed | Real‐time imaging of dynamic cell reprogramming with nanosensors |
| title_short | Real‐time imaging of dynamic cell reprogramming with nanosensors |
| title_sort | real time imaging of dynamic cell reprogramming with nanosensors |
| topic | Engineering::Chemical engineering Cell Reprogramming Molecular Beacons |
| url | https://hdl.handle.net/10356/139022 |
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