CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae

CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae

In this study, we focused on the applicability of CRISPR/Cpf1 in genome simplification of Saccharomyces cerevisiae and established a CRISPR/Cpf1 assisted method for rapid markerless large fragment deletion to facilitate laboratory evolution of geome of S. cerevisiae by rational genetic engineering....

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Bibliographic Details
Main Authors: Li, Zhen-Hai, Liu, Min, Lyu, Xiao-Mei, Wang, Feng-Qing, Wei, Dong-Zhi
Other Authors: School of Chemical and Biomedical Engineering
Format: Journal Article
Language:English
Published: 2020
Subjects:
Engineering::Bioengineering
CRISPR/Cpf1
Large Fragment Deletion
Online Access:https://hdl.handle.net/10356/139529

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