Polyamine regulator AMD1 promotes cell migration in epidermal wound healing
Wound healing is a dynamic process involving gene-expression changes that drive re-epithelialization. Here, we describe an essential role for polyamine regulator AMD1 in driving cell migration at the wound edge. The polyamines, putrescine, spermidine, and spermine are small cationic molecules that p...
| 主要な著者: | , , , , , , , , |
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| その他の著者: | |
| フォーマット: | Journal Article |
| 言語: | English |
| 出版事項: |
2020
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| 主題: | |
| オンライン･アクセス: | https://hdl.handle.net/10356/139584 |
| _version_ | 1826115718760890368 |
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| author | Lim, Hui Kheng Anisa Rahim Leo, Vonny Ivon Das, Shatarupa Lim, Thiam Chye Uemura, Takeshi Igarashi, Kazuei Common, John Vardy, Leah Anne |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Lim, Hui Kheng Anisa Rahim Leo, Vonny Ivon Das, Shatarupa Lim, Thiam Chye Uemura, Takeshi Igarashi, Kazuei Common, John Vardy, Leah Anne |
| author_sort | Lim, Hui Kheng |
| collection | NTU |
| description | Wound healing is a dynamic process involving gene-expression changes that drive re-epithelialization. Here, we describe an essential role for polyamine regulator AMD1 in driving cell migration at the wound edge. The polyamines, putrescine, spermidine, and spermine are small cationic molecules that play essential roles in many cellular processes. We demonstrate that AMD1 is rapidly upregulated following wounding in human skin biopsies. Knockdown of AMD1 with small hairpin RNAs causes a delay in cell migration that is rescued by the addition of spermine. We further show that spermine can promote cell migration in keratinocytes and in human ex vivo wounds, where it significantly increases epithelial tongue migration. Knockdown of AMD1 prevents the upregulation of urokinase-type plasminogen activator/urokinase-type plasminogen activator receptor on wounding and results in a failure in actin cytoskeletal reorganization at the wound edge. We demonstrate that keratinocytes respond to wounding by modulating polyamine regulator AMD1 in order to regulate downstream gene expression and promote cell migration. This article highlights a previously unreported role for the regulation of polyamine levels and ratios in cellular behavior and fate. |
| first_indexed | 2024-10-01T03:59:44Z |
| format | Journal Article |
| id | ntu-10356/139584 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:59:44Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1395842020-05-20T07:06:18Z Polyamine regulator AMD1 promotes cell migration in epidermal wound healing Lim, Hui Kheng Anisa Rahim Leo, Vonny Ivon Das, Shatarupa Lim, Thiam Chye Uemura, Takeshi Igarashi, Kazuei Common, John Vardy, Leah Anne School of Biological Sciences Science::Medicine AMD1 Cell Migration Wound healing is a dynamic process involving gene-expression changes that drive re-epithelialization. Here, we describe an essential role for polyamine regulator AMD1 in driving cell migration at the wound edge. The polyamines, putrescine, spermidine, and spermine are small cationic molecules that play essential roles in many cellular processes. We demonstrate that AMD1 is rapidly upregulated following wounding in human skin biopsies. Knockdown of AMD1 with small hairpin RNAs causes a delay in cell migration that is rescued by the addition of spermine. We further show that spermine can promote cell migration in keratinocytes and in human ex vivo wounds, where it significantly increases epithelial tongue migration. Knockdown of AMD1 prevents the upregulation of urokinase-type plasminogen activator/urokinase-type plasminogen activator receptor on wounding and results in a failure in actin cytoskeletal reorganization at the wound edge. We demonstrate that keratinocytes respond to wounding by modulating polyamine regulator AMD1 in order to regulate downstream gene expression and promote cell migration. This article highlights a previously unreported role for the regulation of polyamine levels and ratios in cellular behavior and fate. ASTAR (Agency for Sci., Tech. and Research, S’pore) 2020-05-20T07:06:18Z 2020-05-20T07:06:18Z 2018 Journal Article Lim, H. K., Anisa Rahim, Leo, V. I., Das, S., Lim, T. C., Uemura, T., . . . Vardy, L. A. (2018). Polyamine regulator AMD1 promotes cell migration in epidermal wound healing. Journal of Investigative Dermatology, 138(12), 2653-2665. doi:10.1016/j.jid.2018.05.029 0022-202X https://hdl.handle.net/10356/139584 10.1016/j.jid.2018.05.029 29906410 2-s2.0-85051485665 12 138 2653 2665 en Journal of Investigative Dermatology © 2018 The Authors (published by Elsevier, Inc. on behalf of the Society for Investigative Dermatology). All rights reserved. |
| spellingShingle | Science::Medicine AMD1 Cell Migration Lim, Hui Kheng Anisa Rahim Leo, Vonny Ivon Das, Shatarupa Lim, Thiam Chye Uemura, Takeshi Igarashi, Kazuei Common, John Vardy, Leah Anne Polyamine regulator AMD1 promotes cell migration in epidermal wound healing |
| title | Polyamine regulator AMD1 promotes cell migration in epidermal wound healing |
| title_full | Polyamine regulator AMD1 promotes cell migration in epidermal wound healing |
| title_fullStr | Polyamine regulator AMD1 promotes cell migration in epidermal wound healing |
| title_full_unstemmed | Polyamine regulator AMD1 promotes cell migration in epidermal wound healing |
| title_short | Polyamine regulator AMD1 promotes cell migration in epidermal wound healing |
| title_sort | polyamine regulator amd1 promotes cell migration in epidermal wound healing |
| topic | Science::Medicine AMD1 Cell Migration |
| url | https://hdl.handle.net/10356/139584 |
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