Sheath-assisted hydrodynamic particle focusing in higher Reynolds number flows
Focusing of sample cells or particles to a single-particle stream in miniature flow cytometers is achieved using pressure-driven hydrodynamic focusing in microfluidic channels. Hydrodynamic focusing models predict the focused sample stream width in the low Reynolds number regime (Re 1) of the Navier...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/10356/139927 |
| _version_ | 1811689026596372480 |
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| author | Panwar, Nishtha Song, Peiyi Tjin, Swee Chuan Yong, Ken-Tye |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Panwar, Nishtha Song, Peiyi Tjin, Swee Chuan Yong, Ken-Tye |
| author_sort | Panwar, Nishtha |
| collection | NTU |
| description | Focusing of sample cells or particles to a single-particle stream in miniature flow cytometers is achieved using pressure-driven hydrodynamic focusing in microfluidic channels. Hydrodynamic focusing models predict the focused sample stream width in the low Reynolds number regime (Re 1) of the Navier-Stokes equations, wherein the viscous forces dominate the inertial forces. Nonetheless, operating in the viscous regime of the laminar microfluidic flow results in high relative focused stream width, and also limits the efficiency of microfluidic flow cytometers as sample throughput is low due to extremely low flow rates. Hence, to enhance the power of microfluidic cell focusing, study of the hydrodynamic focusing mechanism at high Re, and thus, the effect of inertial forces in sheath-assisted flows is required. This work presents a comparative analysis of sheath-assisted hydrodynamic particle focusing in both the viscous and inertial regimes. Experimental results for pressure-driven hydrodynamic focusing inside microchannels in the higher Re (60 < Re < 130) laminar regime are presented along with particle trajectory simulations. Furthermore, we present a comparison of the focusing performance of sheath-assisted hydrodynamic focusing at lower and higher Re. These studies underline the conditions for single-particle focusing for a range of flow parameters and relative particle sizes, particularly for microfluidic flow cytometry. Such analyses constitute an essential aspect of engineering miniature flow cytometers or other cell manipulation techniques. |
| first_indexed | 2024-10-01T05:41:33Z |
| format | Journal Article |
| id | ntu-10356/139927 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:41:33Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1399272020-05-22T08:49:43Z Sheath-assisted hydrodynamic particle focusing in higher Reynolds number flows Panwar, Nishtha Song, Peiyi Tjin, Swee Chuan Yong, Ken-Tye School of Electrical and Electronic Engineering Engineering::Electrical and electronic engineering Hydrodynamic Focusing Sheath Flow Focusing of sample cells or particles to a single-particle stream in miniature flow cytometers is achieved using pressure-driven hydrodynamic focusing in microfluidic channels. Hydrodynamic focusing models predict the focused sample stream width in the low Reynolds number regime (Re 1) of the Navier-Stokes equations, wherein the viscous forces dominate the inertial forces. Nonetheless, operating in the viscous regime of the laminar microfluidic flow results in high relative focused stream width, and also limits the efficiency of microfluidic flow cytometers as sample throughput is low due to extremely low flow rates. Hence, to enhance the power of microfluidic cell focusing, study of the hydrodynamic focusing mechanism at high Re, and thus, the effect of inertial forces in sheath-assisted flows is required. This work presents a comparative analysis of sheath-assisted hydrodynamic particle focusing in both the viscous and inertial regimes. Experimental results for pressure-driven hydrodynamic focusing inside microchannels in the higher Re (60 < Re < 130) laminar regime are presented along with particle trajectory simulations. Furthermore, we present a comparison of the focusing performance of sheath-assisted hydrodynamic focusing at lower and higher Re. These studies underline the conditions for single-particle focusing for a range of flow parameters and relative particle sizes, particularly for microfluidic flow cytometry. Such analyses constitute an essential aspect of engineering miniature flow cytometers or other cell manipulation techniques. MOE (Min. of Education, S’pore) 2020-05-22T08:49:42Z 2020-05-22T08:49:42Z 2018 Journal Article Panwar, N., Song, P., Tjin, S. C., & Yong, K.-T. (2018). Sheath-assisted hydrodynamic particle focusing in higher Reynolds number flows. Journal of Micromechanics and Microengineering, 28, 105018-. doi:10.1088/1361-6439/aad493 0960-1317 https://hdl.handle.net/10356/139927 10.1088/1361-6439/aad493 2-s2.0-85052501217 28 en Journal of Micromechanics and Microengineering © 2018 IOP Publishing Ltd. All rights reserved. This is an author-created, un-copyedited version of an article accepted for publication in Journal of Micromechanics and Microengineering. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The definitive publisher authenticated version is available online at https://doi.org/10.1088/1361-6439/aad493 |
| spellingShingle | Engineering::Electrical and electronic engineering Hydrodynamic Focusing Sheath Flow Panwar, Nishtha Song, Peiyi Tjin, Swee Chuan Yong, Ken-Tye Sheath-assisted hydrodynamic particle focusing in higher Reynolds number flows |
| title | Sheath-assisted hydrodynamic particle focusing in higher Reynolds number flows |
| title_full | Sheath-assisted hydrodynamic particle focusing in higher Reynolds number flows |
| title_fullStr | Sheath-assisted hydrodynamic particle focusing in higher Reynolds number flows |
| title_full_unstemmed | Sheath-assisted hydrodynamic particle focusing in higher Reynolds number flows |
| title_short | Sheath-assisted hydrodynamic particle focusing in higher Reynolds number flows |
| title_sort | sheath assisted hydrodynamic particle focusing in higher reynolds number flows |
| topic | Engineering::Electrical and electronic engineering Hydrodynamic Focusing Sheath Flow |
| url | https://hdl.handle.net/10356/139927 |
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