Impact of inorganic salts on degradation of bisphenol A and diclofenac by crude extracellular enzyme from Pleurotus ostreatus
This study investigated the influence of inorganic salts on enzymatic activity and the removal of trace organic contaminants (TrOCs) by crude laccase from the white-rot fungus Pleurotus ostreatus. A systematic analysis of 15 cations and anions from common inorganic salts was presented. Laccase activ...
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| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/10356/140706 |
| _version_ | 1826112660281753600 |
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| author | Chapple, Alexander Nguyen, Luong N. Hai, Faisal I. Dosseto, Anthony Rashid, Md. Harun-Or Oh, Seungdae Price, William E. Nghiem, Long D. |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Chapple, Alexander Nguyen, Luong N. Hai, Faisal I. Dosseto, Anthony Rashid, Md. Harun-Or Oh, Seungdae Price, William E. Nghiem, Long D. |
| author_sort | Chapple, Alexander |
| collection | NTU |
| description | This study investigated the influence of inorganic salts on enzymatic activity and the removal of trace organic contaminants (TrOCs) by crude laccase from the white-rot fungus Pleurotus ostreatus. A systematic analysis of 15 cations and anions from common inorganic salts was presented. Laccase activity was not inhibited by monovalent cations (i.e. Na+, NH4+, K+), while the presence of divalent and trivalent cations showed variable impact–from negligible to complete inhibition–of both laccase activity and its TrOC removal performance. Of interest was the observation of discrepancy between residual laccase activity and TrOC removal in the presence of some ions. Mg 2+ had negligible impact on residual laccase activity but significant impact on TrOC removal. Conversely, F− showed greater impact on residual laccase activity than on TrOC removal. This observation indicated different impacts of the interfering ions on the interaction between laccase and TrOCs as compared to that between laccase and the reagent used to measure its activity, implicating that residual laccase activity may not always be an accurate indicator of TrOC removal. The degree of impact of halides was in the order of F−>I−>Br−>Cl−. Particularly, the tolerance of the tested laccase to Cl− has important implications for a range of industrial applications. |
| first_indexed | 2024-10-01T03:10:25Z |
| format | Journal Article |
| id | ntu-10356/140706 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:10:25Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1407062020-06-01T08:21:39Z Impact of inorganic salts on degradation of bisphenol A and diclofenac by crude extracellular enzyme from Pleurotus ostreatus Chapple, Alexander Nguyen, Luong N. Hai, Faisal I. Dosseto, Anthony Rashid, Md. Harun-Or Oh, Seungdae Price, William E. Nghiem, Long D. School of Civil and Environmental Engineering Engineering::Civil engineering Laccase Trace Organic Contaminants This study investigated the influence of inorganic salts on enzymatic activity and the removal of trace organic contaminants (TrOCs) by crude laccase from the white-rot fungus Pleurotus ostreatus. A systematic analysis of 15 cations and anions from common inorganic salts was presented. Laccase activity was not inhibited by monovalent cations (i.e. Na+, NH4+, K+), while the presence of divalent and trivalent cations showed variable impact–from negligible to complete inhibition–of both laccase activity and its TrOC removal performance. Of interest was the observation of discrepancy between residual laccase activity and TrOC removal in the presence of some ions. Mg 2+ had negligible impact on residual laccase activity but significant impact on TrOC removal. Conversely, F− showed greater impact on residual laccase activity than on TrOC removal. This observation indicated different impacts of the interfering ions on the interaction between laccase and TrOCs as compared to that between laccase and the reagent used to measure its activity, implicating that residual laccase activity may not always be an accurate indicator of TrOC removal. The degree of impact of halides was in the order of F−>I−>Br−>Cl−. Particularly, the tolerance of the tested laccase to Cl− has important implications for a range of industrial applications. 2020-06-01T08:21:39Z 2020-06-01T08:21:39Z 2017 Journal Article Chapple, A., Nguyen, L. N., Hai, F. I., Dosseto, A., Rashid, M. H.-O., Oh, S., . . . Nghiem, L. D. (2019). Impact of inorganic salts on degradation of bisphenol a and diclofenac by crude extracellular enzyme from Pleurotus ostreatus. Biocatalysis and Biotransformation, 37(1), 10-17. doi:10.1080/10242422.2017.141533 1024-2422 https://hdl.handle.net/10356/140706 10.1080/10242422.2017.1415332 2-s2.0-85038078437 1 37 10 17 en Biocatalysis and Biotransformation This is an Accepted Manuscript of an article published by Informa UK Limited, trading as Taylor and Francis in Biocatalysis and Biotransformation on 14 Dec 2017, available online: http://www.tandfonline.com/10.1080/10242422.2017.1415332 |
| spellingShingle | Engineering::Civil engineering Laccase Trace Organic Contaminants Chapple, Alexander Nguyen, Luong N. Hai, Faisal I. Dosseto, Anthony Rashid, Md. Harun-Or Oh, Seungdae Price, William E. Nghiem, Long D. Impact of inorganic salts on degradation of bisphenol A and diclofenac by crude extracellular enzyme from Pleurotus ostreatus |
| title | Impact of inorganic salts on degradation of bisphenol A and diclofenac by crude extracellular enzyme from Pleurotus ostreatus |
| title_full | Impact of inorganic salts on degradation of bisphenol A and diclofenac by crude extracellular enzyme from Pleurotus ostreatus |
| title_fullStr | Impact of inorganic salts on degradation of bisphenol A and diclofenac by crude extracellular enzyme from Pleurotus ostreatus |
| title_full_unstemmed | Impact of inorganic salts on degradation of bisphenol A and diclofenac by crude extracellular enzyme from Pleurotus ostreatus |
| title_short | Impact of inorganic salts on degradation of bisphenol A and diclofenac by crude extracellular enzyme from Pleurotus ostreatus |
| title_sort | impact of inorganic salts on degradation of bisphenol a and diclofenac by crude extracellular enzyme from pleurotus ostreatus |
| topic | Engineering::Civil engineering Laccase Trace Organic Contaminants |
| url | https://hdl.handle.net/10356/140706 |
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