Bioprospecting a medicinal plant for therapeutics
Eleutherococcus trifoliatus, a valuable medicinal plant, has been used to treat injuries and diseases for centuries. Its orally active decoction may have the presence of cysteine-rich peptides (CRPs), a 2-6 kDa, functionally diverse and hyperdisulfide mini-proteins that have therapeutic relevance. T...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2020
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| Online Access: | https://hdl.handle.net/10356/140947 |
| _version_ | 1826122468037754880 |
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| author | Ho, Malcolm Zheng Hao |
| author2 | James P Tam |
| author_facet | James P Tam Ho, Malcolm Zheng Hao |
| author_sort | Ho, Malcolm Zheng Hao |
| collection | NTU |
| description | Eleutherococcus trifoliatus, a valuable medicinal plant, has been used to treat injuries and diseases for centuries. Its orally active decoction may have the presence of cysteine-rich peptides (CRPs), a 2-6 kDa, functionally diverse and hyperdisulfide mini-proteins that have therapeutic relevance. This study reports the discovery, characterisation and therapeutic bioprospection of a CRP termed eleutherotide eT3670 extracted from E. trifoliatus. eT3670 consists of 35 amino acids and possesses an 8C-cysteine motif potentially forming six inter- cysteine loops via four intramolecular disulfide bonds that confer it resistance to thermal, acidic, enzymatic and serum-mediated degradation. Transcriptomic and bioinformatics analyses revealed that eT3670 is synthesised as a three-domain precursor, its disulfide connectivity as Cys I-IV, II-VI, III-VII, V-VIII, its inter-cysteine loops tolerance to amino acid variation and that it contains a variant LC3-interacting region motif related to selective autophagy. Cell-based assays showed that eT3670 is cytoprotective and a potential adaptogen that may induce autophagy to maintain cellular homeostasis, protecting cells from hypoxia stress-mediated damage and cell death. Together, the study bioprospects eT3670 as a potential candidate to be developed as a stable orally active drug for the treatment of autophagy-related diseases and as a grafting scaffold to enhance the stability of peptidyl therapeutics. |
| first_indexed | 2024-10-01T05:48:39Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/140947 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:48:39Z |
| publishDate | 2020 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1409472023-02-28T18:08:38Z Bioprospecting a medicinal plant for therapeutics Ho, Malcolm Zheng Hao James P Tam School of Biological Sciences JPTam@ntu.edu.sg Science::Biological sciences Eleutherococcus trifoliatus, a valuable medicinal plant, has been used to treat injuries and diseases for centuries. Its orally active decoction may have the presence of cysteine-rich peptides (CRPs), a 2-6 kDa, functionally diverse and hyperdisulfide mini-proteins that have therapeutic relevance. This study reports the discovery, characterisation and therapeutic bioprospection of a CRP termed eleutherotide eT3670 extracted from E. trifoliatus. eT3670 consists of 35 amino acids and possesses an 8C-cysteine motif potentially forming six inter- cysteine loops via four intramolecular disulfide bonds that confer it resistance to thermal, acidic, enzymatic and serum-mediated degradation. Transcriptomic and bioinformatics analyses revealed that eT3670 is synthesised as a three-domain precursor, its disulfide connectivity as Cys I-IV, II-VI, III-VII, V-VIII, its inter-cysteine loops tolerance to amino acid variation and that it contains a variant LC3-interacting region motif related to selective autophagy. Cell-based assays showed that eT3670 is cytoprotective and a potential adaptogen that may induce autophagy to maintain cellular homeostasis, protecting cells from hypoxia stress-mediated damage and cell death. Together, the study bioprospects eT3670 as a potential candidate to be developed as a stable orally active drug for the treatment of autophagy-related diseases and as a grafting scaffold to enhance the stability of peptidyl therapeutics. Bachelor of Science in Biological Sciences 2020-06-03T03:15:00Z 2020-06-03T03:15:00Z 2020 Final Year Project (FYP) https://hdl.handle.net/10356/140947 en application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Ho, Malcolm Zheng Hao Bioprospecting a medicinal plant for therapeutics |
| title | Bioprospecting a medicinal plant for therapeutics |
| title_full | Bioprospecting a medicinal plant for therapeutics |
| title_fullStr | Bioprospecting a medicinal plant for therapeutics |
| title_full_unstemmed | Bioprospecting a medicinal plant for therapeutics |
| title_short | Bioprospecting a medicinal plant for therapeutics |
| title_sort | bioprospecting a medicinal plant for therapeutics |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/140947 |
| work_keys_str_mv | AT homalcolmzhenghao bioprospectingamedicinalplantfortherapeutics |