Interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces
The pathway of vesicle adsorption onto a solid support depends on the material composition of the underlying support, and there is significant interest in developing material-independent strategies to modulate the spectrum of vesicle-substrate interactions on a particular surface. Herein, using the...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/141541 |
| _version_ | 1811695247604842496 |
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| author | Biswas, Kabir Hassan Jackman, Joshua Alexander Park, Jae Hyeon Groves, Jay T. Cho, Nam-Joon |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Biswas, Kabir Hassan Jackman, Joshua Alexander Park, Jae Hyeon Groves, Jay T. Cho, Nam-Joon |
| author_sort | Biswas, Kabir Hassan |
| collection | NTU |
| description | The pathway of vesicle adsorption onto a solid support depends on the material composition of the underlying support, and there is significant interest in developing material-independent strategies to modulate the spectrum of vesicle-substrate interactions on a particular surface. Herein, using the quartz crystal microbalance-dissipation (QCM-D) technique, we systematically investigated how solution pH and membrane surface charge affect vesicle adsorption onto a silicon dioxide surface. While vesicle adsorption and spontaneous rupture to form complete supported lipid bilayer (SLBs) occurred in acidic conditions, it was discovered that a wide range of adsorption pathways occurred in alkaline conditions, including (i) vesicle adsorption and spontaneous rupture to form complete SLBs, (ii) vesicle adsorption and spontaneous rupture to form incomplete SLBs, (iii) irreversible adsorption of intact vesicles, (iv) reversible adsorption of intact vesicles, and (v) negligible adsorption. In general, SLB formation became more favorable with increasingly positive membrane surface charge although there were certain conditions at which attractive electrostatic forces were insufficient to promote vesicle rupture. To rationalize these findings, we discuss how solution pH and membrane surface charge affect interfacial forces involved in vesicle-substrate interactions. Taken together, our findings present a comprehensive picture of how interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces and offer a broadly applicable framework to characterize the interactions between phospholipid vesicles and inorganic material surfaces. |
| first_indexed | 2024-10-01T07:20:26Z |
| format | Journal Article |
| id | ntu-10356/141541 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:20:26Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1415412020-06-09T03:40:45Z Interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces Biswas, Kabir Hassan Jackman, Joshua Alexander Park, Jae Hyeon Groves, Jay T. Cho, Nam-Joon School of Chemical and Biomedical Engineering School of Materials Science and Engineering Engineering::Materials Vesicles Lipids The pathway of vesicle adsorption onto a solid support depends on the material composition of the underlying support, and there is significant interest in developing material-independent strategies to modulate the spectrum of vesicle-substrate interactions on a particular surface. Herein, using the quartz crystal microbalance-dissipation (QCM-D) technique, we systematically investigated how solution pH and membrane surface charge affect vesicle adsorption onto a silicon dioxide surface. While vesicle adsorption and spontaneous rupture to form complete supported lipid bilayer (SLBs) occurred in acidic conditions, it was discovered that a wide range of adsorption pathways occurred in alkaline conditions, including (i) vesicle adsorption and spontaneous rupture to form complete SLBs, (ii) vesicle adsorption and spontaneous rupture to form incomplete SLBs, (iii) irreversible adsorption of intact vesicles, (iv) reversible adsorption of intact vesicles, and (v) negligible adsorption. In general, SLB formation became more favorable with increasingly positive membrane surface charge although there were certain conditions at which attractive electrostatic forces were insufficient to promote vesicle rupture. To rationalize these findings, we discuss how solution pH and membrane surface charge affect interfacial forces involved in vesicle-substrate interactions. Taken together, our findings present a comprehensive picture of how interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces and offer a broadly applicable framework to characterize the interactions between phospholipid vesicles and inorganic material surfaces. NRF (Natl Research Foundation, S’pore) 2020-06-09T03:40:45Z 2020-06-09T03:40:45Z 2017 Journal Article Biswas, K. H., Jackman, J. A., Park, J. H., Groves, J. T., & Cho, N.-J. (2018). Interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces. Langmuir, 34(4), 1775-1782. doi:10.1021/acs.langmuir.7b03799 0743-7463 https://hdl.handle.net/10356/141541 10.1021/acs.langmuir.7b03799 29281791 2-s2.0-85041444673 4 34 1775 1782 en Langmuir © 2017 American Chemical Society. All rights reserved. |
| spellingShingle | Engineering::Materials Vesicles Lipids Biswas, Kabir Hassan Jackman, Joshua Alexander Park, Jae Hyeon Groves, Jay T. Cho, Nam-Joon Interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces |
| title | Interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces |
| title_full | Interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces |
| title_fullStr | Interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces |
| title_full_unstemmed | Interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces |
| title_short | Interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces |
| title_sort | interfacial forces dictate the pathway of phospholipid vesicle adsorption onto silicon dioxide surfaces |
| topic | Engineering::Materials Vesicles Lipids |
| url | https://hdl.handle.net/10356/141541 |
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