Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations
Scope: The purpose of this study is to identify and visualize the spatial distribution of proteins present in amyloid corneal deposits of TGFBI‐CD patients using Mass Spectrometry Imaging (MSI) and compare it with healthy control cornea. Corneal Dystrophies (CD) constitute a group of genetically inh...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/10356/141816 |
| _version_ | 1826113194394910720 |
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| author | Venkatraman, Anandalakshmi Hochart, Guillaume Bonnel, David Stauber, Jonathan Shimmura, Shigeto Rajamani, Lakshminaryanan Pervushin, Konstantin Mehta, Jodhbir S. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Venkatraman, Anandalakshmi Hochart, Guillaume Bonnel, David Stauber, Jonathan Shimmura, Shigeto Rajamani, Lakshminaryanan Pervushin, Konstantin Mehta, Jodhbir S. |
| author_sort | Venkatraman, Anandalakshmi |
| collection | NTU |
| description | Scope: The purpose of this study is to identify and visualize the spatial distribution of proteins present in amyloid corneal deposits of TGFBI‐CD patients using Mass Spectrometry Imaging (MSI) and compare it with healthy control cornea. Corneal Dystrophies (CD) constitute a group of genetically inherited protein aggregation disorders that affects different layers of the cornea. With accumulated protein deposition, the cornea becomes opaque with decreased visual acuity. CD affecting the stroma and Bowman's membrane, is associated with mutations in transforming growth factor β‐induced (TGFBI ) gene. Methods: MALDI‐Mass Spectrometry Imaging (MSI) is performed on 2 patient corneas and is compared with 1 healthy control cornea using a 7T‐MALDI‐FTICR. Molecular images obtained are overlaid with congo‐red stained sections to visualize the proteins associated with the corneal amyloid aggregates. Results: MALDI‐MSI provides a relative abundance and two dimensional spatial protein signature of key proteins (TGFBIp, Apolipoprotein A‐I, Apolipoprotein A‐IV, Apolipoprotein E, Kaliocin‐1, Pyruvate Kinase and Ras related protein Rab‐10) in the patient deposits compared to the control. This is the first report of the anatomical localization of key proteins on corneal tissue section from CD patients. This may provide insight in understanding the mechanism of amyloid fibril formation in TGFBI ‐corneal dystrophy. |
| first_indexed | 2024-10-01T03:19:12Z |
| format | Journal Article |
| id | ntu-10356/141816 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:19:12Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1418162020-06-11T01:57:23Z Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations Venkatraman, Anandalakshmi Hochart, Guillaume Bonnel, David Stauber, Jonathan Shimmura, Shigeto Rajamani, Lakshminaryanan Pervushin, Konstantin Mehta, Jodhbir S. School of Biological Sciences Science::Biological sciences Corneal Dystrophy Matrix-assisted Laser Desorption/Ionization Scope: The purpose of this study is to identify and visualize the spatial distribution of proteins present in amyloid corneal deposits of TGFBI‐CD patients using Mass Spectrometry Imaging (MSI) and compare it with healthy control cornea. Corneal Dystrophies (CD) constitute a group of genetically inherited protein aggregation disorders that affects different layers of the cornea. With accumulated protein deposition, the cornea becomes opaque with decreased visual acuity. CD affecting the stroma and Bowman's membrane, is associated with mutations in transforming growth factor β‐induced (TGFBI ) gene. Methods: MALDI‐Mass Spectrometry Imaging (MSI) is performed on 2 patient corneas and is compared with 1 healthy control cornea using a 7T‐MALDI‐FTICR. Molecular images obtained are overlaid with congo‐red stained sections to visualize the proteins associated with the corneal amyloid aggregates. Results: MALDI‐MSI provides a relative abundance and two dimensional spatial protein signature of key proteins (TGFBIp, Apolipoprotein A‐I, Apolipoprotein A‐IV, Apolipoprotein E, Kaliocin‐1, Pyruvate Kinase and Ras related protein Rab‐10) in the patient deposits compared to the control. This is the first report of the anatomical localization of key proteins on corneal tissue section from CD patients. This may provide insight in understanding the mechanism of amyloid fibril formation in TGFBI ‐corneal dystrophy. 2020-06-11T01:57:23Z 2020-06-11T01:57:23Z 2018 Journal Article Venkatraman, A., Hochart, G., Bonnel, D., Stauber, J., Shimmura, S., Rajamani, L., . . . Mehta, J. S. (2019). Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations. PROTEOMICS – Clinical Applications, 13(1), 1800053-. doi:10.1002/prca.201800053 1862-8346 https://hdl.handle.net/10356/141816 10.1002/prca.201800053 30387319 2-s2.0-85056719525 1 13 en PROTEOMICS – Clinical Applications © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved. |
| spellingShingle | Science::Biological sciences Corneal Dystrophy Matrix-assisted Laser Desorption/Ionization Venkatraman, Anandalakshmi Hochart, Guillaume Bonnel, David Stauber, Jonathan Shimmura, Shigeto Rajamani, Lakshminaryanan Pervushin, Konstantin Mehta, Jodhbir S. Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations |
| title | Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations |
| title_full | Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations |
| title_fullStr | Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations |
| title_full_unstemmed | Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations |
| title_short | Matrix-assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with TGFBI-H626R and TGFBI-R124C mutations |
| title_sort | matrix assisted laser desorption ionization mass spectrometry imaging of key proteins in corneal samples from lattice dystrophy patients with tgfbi h626r and tgfbi r124c mutations |
| topic | Science::Biological sciences Corneal Dystrophy Matrix-assisted Laser Desorption/Ionization |
| url | https://hdl.handle.net/10356/141816 |
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