Fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes
Background: Preferential utilization of fatty acids for ATP production represents an advanced metabolic phenotype in developing cardiomyocytes. We investigated whether this phenotype could be attained in human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) and assessed its influenc...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/10356/142041 |
| _version_ | 1811686854248890368 |
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| author | Ramachandra, Chrishan J. A. Mehta, Ashish Wong, Philip Ja, K. P. Myu Mai Fritsche-Danielson, Regina Bhat, Ratan V. Hausenloy, Derek J. Kovalik, Jean-Paul Shim, Winston |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Ramachandra, Chrishan J. A. Mehta, Ashish Wong, Philip Ja, K. P. Myu Mai Fritsche-Danielson, Regina Bhat, Ratan V. Hausenloy, Derek J. Kovalik, Jean-Paul Shim, Winston |
| author_sort | Ramachandra, Chrishan J. A. |
| collection | NTU |
| description | Background: Preferential utilization of fatty acids for ATP production represents an advanced metabolic phenotype in developing cardiomyocytes. We investigated whether this phenotype could be attained in human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) and assessed its influence on mitochondrial morphology, bioenergetics, respiratory capacity and ultra-structural architecture. Methods and results: Whole-cell proteome analysis of day 14 and day 30-CMs maintained in glucose media revealed a positive influence of extended culture on mitochondria-related processes that primed the day 30-CMs for fatty acid metabolism. Supplementing the day 30-CMs with palmitate/oleate (fatty acids) significantly enhanced mitochondrial remodeling, oxygen consumption rates and ATP production. Metabolomic analysis upon fatty acid supplementation revealed a β-oxidation fueled ATP elevation that coincided with presence of junctional complexes, intercalated discs, t-tubule-like structures and adult isoform of cardiac troponin T. In contrast, glucose-maintained day 30-CMs continued to harbor underdeveloped ultra-structural architecture and more subdued bioenergetics, constrained by suboptimal mitochondria development. Conclusion: The advanced metabolic phenotype of preferential fatty acid utilization was attained in hiPSC-CMs, whereby fatty acid driven β-oxidation sustained cardiac bioenergetics and respiratory capacity resulting in ultra-structural and functional characteristics similar to those of developmentally advanced cardiomyocytes. Better understanding of mitochondrial bioenergetics and ultra-structural adaptation associated with fatty acid metabolism has important implications in the study of cardiac physiology that are associated with late-onset mitochondrial and metabolic adaptations. |
| first_indexed | 2024-10-01T05:07:02Z |
| format | Journal Article |
| id | ntu-10356/142041 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:07:02Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1420412020-06-15T04:32:00Z Fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes Ramachandra, Chrishan J. A. Mehta, Ashish Wong, Philip Ja, K. P. Myu Mai Fritsche-Danielson, Regina Bhat, Ratan V. Hausenloy, Derek J. Kovalik, Jean-Paul Shim, Winston School of Materials Science and Engineering Engineering::Materials Mitochondria Metabolism Background: Preferential utilization of fatty acids for ATP production represents an advanced metabolic phenotype in developing cardiomyocytes. We investigated whether this phenotype could be attained in human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) and assessed its influence on mitochondrial morphology, bioenergetics, respiratory capacity and ultra-structural architecture. Methods and results: Whole-cell proteome analysis of day 14 and day 30-CMs maintained in glucose media revealed a positive influence of extended culture on mitochondria-related processes that primed the day 30-CMs for fatty acid metabolism. Supplementing the day 30-CMs with palmitate/oleate (fatty acids) significantly enhanced mitochondrial remodeling, oxygen consumption rates and ATP production. Metabolomic analysis upon fatty acid supplementation revealed a β-oxidation fueled ATP elevation that coincided with presence of junctional complexes, intercalated discs, t-tubule-like structures and adult isoform of cardiac troponin T. In contrast, glucose-maintained day 30-CMs continued to harbor underdeveloped ultra-structural architecture and more subdued bioenergetics, constrained by suboptimal mitochondria development. Conclusion: The advanced metabolic phenotype of preferential fatty acid utilization was attained in hiPSC-CMs, whereby fatty acid driven β-oxidation sustained cardiac bioenergetics and respiratory capacity resulting in ultra-structural and functional characteristics similar to those of developmentally advanced cardiomyocytes. Better understanding of mitochondrial bioenergetics and ultra-structural adaptation associated with fatty acid metabolism has important implications in the study of cardiac physiology that are associated with late-onset mitochondrial and metabolic adaptations. 2020-06-15T04:32:00Z 2020-06-15T04:32:00Z 2018 Journal Article Ramachandra, C. J. A., Mehta, A., Wong, P., Ja, K. P. M. M., Fritsche-Danielson, R., Bhat, R. V., . . . Shim, W. (2018). Fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes. International journal of cardiology, 272, 288-297. doi:10.1016/j.ijcard.2018.08.069 0167-5273 https://hdl.handle.net/10356/142041 10.1016/j.ijcard.2018.08.069 30177232 2-s2.0-85052753562 272 288 297 en International journal of cardiology © 2018 Elsevier B.V. All rights reserved. |
| spellingShingle | Engineering::Materials Mitochondria Metabolism Ramachandra, Chrishan J. A. Mehta, Ashish Wong, Philip Ja, K. P. Myu Mai Fritsche-Danielson, Regina Bhat, Ratan V. Hausenloy, Derek J. Kovalik, Jean-Paul Shim, Winston Fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes |
| title | Fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes |
| title_full | Fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes |
| title_fullStr | Fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes |
| title_full_unstemmed | Fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes |
| title_short | Fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes |
| title_sort | fatty acid metabolism driven mitochondrial bioenergetics promotes advanced developmental phenotypes in human induced pluripotent stem cell derived cardiomyocytes |
| topic | Engineering::Materials Mitochondria Metabolism |
| url | https://hdl.handle.net/10356/142041 |
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