Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules
Two separate structural elements of a G-quadruplex (G4), a vacant site and a flanking single-strand, provide an opportunity for specific targeting of a particular G4 structure via dual recognition. Here, we show that a short peptide nucleic acid (PNA) can specifically recognize and bind to a G4 at s...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/142097 |
| _version_ | 1826117968844554240 |
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| author | Tan, Derrick Jing Yang Das, Poulomi Winnerdy, Fernaldo Richtia Lim, Kah Wai Phan, Anh Tuân |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Tan, Derrick Jing Yang Das, Poulomi Winnerdy, Fernaldo Richtia Lim, Kah Wai Phan, Anh Tuân |
| author_sort | Tan, Derrick Jing Yang |
| collection | NTU |
| description | Two separate structural elements of a G-quadruplex (G4), a vacant site and a flanking single-strand, provide an opportunity for specific targeting of a particular G4 structure via dual recognition. Here, we show that a short peptide nucleic acid (PNA) can specifically recognize and bind to a G4 at sub-micromolar affinity based on both G-tetrad vacant site filling and complementary duplex formation. This sequence-guided guanine-anchoring strategy can be further developed for specific targeting of G4 structures using short DNA, LNA and PNA strands. |
| first_indexed | 2024-10-01T04:36:03Z |
| format | Journal Article |
| id | ntu-10356/142097 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:36:03Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1420972023-02-28T19:48:20Z Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules Tan, Derrick Jing Yang Das, Poulomi Winnerdy, Fernaldo Richtia Lim, Kah Wai Phan, Anh Tuân School of Physical and Mathematical Sciences NTU Institute of Structural Biology Science::Chemistry Science::Biological sciences PNA DNA Two separate structural elements of a G-quadruplex (G4), a vacant site and a flanking single-strand, provide an opportunity for specific targeting of a particular G4 structure via dual recognition. Here, we show that a short peptide nucleic acid (PNA) can specifically recognize and bind to a G4 at sub-micromolar affinity based on both G-tetrad vacant site filling and complementary duplex formation. This sequence-guided guanine-anchoring strategy can be further developed for specific targeting of G4 structures using short DNA, LNA and PNA strands. NRF (Natl Research Foundation, S’pore) Accepted version 2020-06-16T00:45:01Z 2020-06-16T00:45:01Z 2020 Journal Article Tan, D. J. Y., Das, P., Winnerdy, F. R., Lim, K. W., & Phan, A. T. (2020). Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules. Chemical Communications, 56(44), 5897-5900. doi:10.1039/d0cc01778g 1359-7345 https://hdl.handle.net/10356/142097 10.1039/d0cc01778g 32338660 44 56 5897 5900 en NRF-NRFI2017-09 Chemical Communications © 2020 The Royal Society of Chemistry. All rights reserved. This paper was published in Chemical Communications and is made available with permission of The Royal Society of Chemistry. application/pdf |
| spellingShingle | Science::Chemistry Science::Biological sciences PNA DNA Tan, Derrick Jing Yang Das, Poulomi Winnerdy, Fernaldo Richtia Lim, Kah Wai Phan, Anh Tuân Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules |
| title | Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules |
| title_full | Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules |
| title_fullStr | Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules |
| title_full_unstemmed | Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules |
| title_short | Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules |
| title_sort | guanine anchoring a strategy for specific targeting of a g quadruplex using short pna lna and dna molecules |
| topic | Science::Chemistry Science::Biological sciences PNA DNA |
| url | https://hdl.handle.net/10356/142097 |
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