Depth sensitive Raman spectroscopy for skin wounds in rodents
Raman spectroscopy has demonstrated its great potential in skin wound assessment. Given that biochemical changes in skin wound healing is a layer dependent process, depth sensitive Raman spectroscopy could enhance the power of Raman spectroscopy in this application. Considering the critical importan...
| Κύριοι συγγραφείς: | , , , , , , |
|---|---|
| Άλλοι συγγραφείς: | |
| Μορφή: | Conference Paper |
| Γλώσσα: | English |
| Έκδοση: |
2020
|
| Θέματα: | |
| Διαθέσιμο Online: | https://hdl.handle.net/10356/142375 |
| _version_ | 1826126053040455680 |
|---|---|
| author | Su, Joshua Weiming Wang, Qiang Tian, Yao Madden, Leigh Teo, Erica Mei Ling Becker, David Laurence Liu, Quan |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Su, Joshua Weiming Wang, Qiang Tian, Yao Madden, Leigh Teo, Erica Mei Ling Becker, David Laurence Liu, Quan |
| author_sort | Su, Joshua Weiming |
| collection | NTU |
| description | Raman spectroscopy has demonstrated its great potential in skin wound assessment. Given that biochemical changes in skin wound healing is a layer dependent process, depth sensitive Raman spectroscopy could enhance the power of Raman spectroscopy in this application. Considering the critical importance of rodent studies in the field of skin wound assessment, it is necessary to develop and validate a system that can perform depth sensitive measurements in rat skin with a proper target depth range. In this manuscript, we report the design, optimization and evaluation of a new snapshot depth-sensitive Raman instrument for rat skin measurements. The optical design and optimization process are presented first. The depth sensitive measurement performance is characterized on ex vivo rat skin samples with wounds. Raman signal emitted by the ex vivo rat skin from different target depths were simultaneously acquired. The feasibility of using the measured Raman spectra to differentiate between the wound edge and healthy skin was validated using PLS-LDA with leave-one-out. The accuracy of the classification improves monotonically as more data from new depths are used, which implies that each depth offers additional information useful for classification. This instrument demonstrates the ability to perform snapshot depth sensitive Raman measurements from rat skin, which paves the way towards in vivo preclinical studies of rat skin wounds. |
| first_indexed | 2024-10-01T06:46:25Z |
| format | Conference Paper |
| id | ntu-10356/142375 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:46:25Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1423752023-12-29T06:44:12Z Depth sensitive Raman spectroscopy for skin wounds in rodents Su, Joshua Weiming Wang, Qiang Tian, Yao Madden, Leigh Teo, Erica Mei Ling Becker, David Laurence Liu, Quan School of Chemical and Biomedical Engineering Interdisciplinary Graduate School (IGS) Lee Kong Chian School of Medicine (LKCMedicine) European Conferences on Biomedical Optics 2019 NTU Institute for Health Technologies, Science::Medicine Raman Spectroscopy Depth Sensitive Raman spectroscopy has demonstrated its great potential in skin wound assessment. Given that biochemical changes in skin wound healing is a layer dependent process, depth sensitive Raman spectroscopy could enhance the power of Raman spectroscopy in this application. Considering the critical importance of rodent studies in the field of skin wound assessment, it is necessary to develop and validate a system that can perform depth sensitive measurements in rat skin with a proper target depth range. In this manuscript, we report the design, optimization and evaluation of a new snapshot depth-sensitive Raman instrument for rat skin measurements. The optical design and optimization process are presented first. The depth sensitive measurement performance is characterized on ex vivo rat skin samples with wounds. Raman signal emitted by the ex vivo rat skin from different target depths were simultaneously acquired. The feasibility of using the measured Raman spectra to differentiate between the wound edge and healthy skin was validated using PLS-LDA with leave-one-out. The accuracy of the classification improves monotonically as more data from new depths are used, which implies that each depth offers additional information useful for classification. This instrument demonstrates the ability to perform snapshot depth sensitive Raman measurements from rat skin, which paves the way towards in vivo preclinical studies of rat skin wounds. Published version 2020-06-19T07:55:53Z 2020-06-19T07:55:53Z 2019 Conference Paper Su, J. W., Wang, Q., Tian, Y., Madden, L., Teo, E. M. L., Becker, D. L., & Liu, Q. (2019). Depth sensitive Raman spectroscopy for skin wounds in rodents. Proceedings of SPIE - Clinical and Preclinical Optical Diagnostics II, 11073, 110731C -. doi:10.1117/12.2526556 9781557528209 https://hdl.handle.net/10356/142375 10.1117/12.2526556 2-s2.0-85084416246 11073 en Copyright 2019 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. application/pdf |
| spellingShingle | Science::Medicine Raman Spectroscopy Depth Sensitive Su, Joshua Weiming Wang, Qiang Tian, Yao Madden, Leigh Teo, Erica Mei Ling Becker, David Laurence Liu, Quan Depth sensitive Raman spectroscopy for skin wounds in rodents |
| title | Depth sensitive Raman spectroscopy for skin wounds in rodents |
| title_full | Depth sensitive Raman spectroscopy for skin wounds in rodents |
| title_fullStr | Depth sensitive Raman spectroscopy for skin wounds in rodents |
| title_full_unstemmed | Depth sensitive Raman spectroscopy for skin wounds in rodents |
| title_short | Depth sensitive Raman spectroscopy for skin wounds in rodents |
| title_sort | depth sensitive raman spectroscopy for skin wounds in rodents |
| topic | Science::Medicine Raman Spectroscopy Depth Sensitive |
| url | https://hdl.handle.net/10356/142375 |
| work_keys_str_mv | AT sujoshuaweiming depthsensitiveramanspectroscopyforskinwoundsinrodents AT wangqiang depthsensitiveramanspectroscopyforskinwoundsinrodents AT tianyao depthsensitiveramanspectroscopyforskinwoundsinrodents AT maddenleigh depthsensitiveramanspectroscopyforskinwoundsinrodents AT teoericameiling depthsensitiveramanspectroscopyforskinwoundsinrodents AT beckerdavidlaurence depthsensitiveramanspectroscopyforskinwoundsinrodents AT liuquan depthsensitiveramanspectroscopyforskinwoundsinrodents |