Rapid microevolution of biofilm cells in response to antibiotics
Infections caused by Acinetobacter baumannii are increasingly antibiotic resistant, generating a significant public health problem. Like many bacteria, A. baumannii adopts a biofilm lifestyle that enhances its antibiotic resistance and environmental resilience. Biofilms represent the predominant mod...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/142631 |
| _version_ | 1811683698054004736 |
|---|---|
| author | Penesyan, Anahit Nagy, Stephanie S. Kjelleberg, Staffan Gillings, Michael R. Paulsen, Ian T. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Penesyan, Anahit Nagy, Stephanie S. Kjelleberg, Staffan Gillings, Michael R. Paulsen, Ian T. |
| author_sort | Penesyan, Anahit |
| collection | NTU |
| description | Infections caused by Acinetobacter baumannii are increasingly antibiotic resistant, generating a significant public health problem. Like many bacteria, A. baumannii adopts a biofilm lifestyle that enhances its antibiotic resistance and environmental resilience. Biofilms represent the predominant mode of microbial life, but research into antibiotic resistance has mainly focused on planktonic cells. We investigated the dynamics of A. baumannii biofilms in the presence of antibiotics. A 3-day exposure of A. baumannii biofilms to sub-inhibitory concentrations of antibiotics had a profound effect, increasing biofilm formation and antibiotic resistance in the majority of biofilm dispersal isolates. Cells dispersing from biofilms were genome sequenced to identify mutations accumulating in their genomes, and network analysis linked these mutations to their phenotypes. Transcriptomics of biofilms confirmed the network analysis results, revealing novel gene functions of relevance to both resistance and biofilm formation. This approach is a rapid and objective tool for investigating resistance dynamics of biofilms. |
| first_indexed | 2024-10-01T04:16:52Z |
| format | Journal Article |
| id | ntu-10356/142631 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:16:52Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1426312023-02-28T17:07:56Z Rapid microevolution of biofilm cells in response to antibiotics Penesyan, Anahit Nagy, Stephanie S. Kjelleberg, Staffan Gillings, Michael R. Paulsen, Ian T. School of Biological Sciences Singapore Centre for Environmental Life Sciences and Engineering Science::Biological sciences Antibiotic Biofilm Infections caused by Acinetobacter baumannii are increasingly antibiotic resistant, generating a significant public health problem. Like many bacteria, A. baumannii adopts a biofilm lifestyle that enhances its antibiotic resistance and environmental resilience. Biofilms represent the predominant mode of microbial life, but research into antibiotic resistance has mainly focused on planktonic cells. We investigated the dynamics of A. baumannii biofilms in the presence of antibiotics. A 3-day exposure of A. baumannii biofilms to sub-inhibitory concentrations of antibiotics had a profound effect, increasing biofilm formation and antibiotic resistance in the majority of biofilm dispersal isolates. Cells dispersing from biofilms were genome sequenced to identify mutations accumulating in their genomes, and network analysis linked these mutations to their phenotypes. Transcriptomics of biofilms confirmed the network analysis results, revealing novel gene functions of relevance to both resistance and biofilm formation. This approach is a rapid and objective tool for investigating resistance dynamics of biofilms. Published version 2020-06-26T00:49:49Z 2020-06-26T00:49:49Z 2019 Journal Article Penesyan, A., Nagy, S. S., Kjelleberg, S., Gillings, M. R., & Paulsen, I. T. (2019). Rapid microevolution of biofilm cells in response to antibiotics. npj Biofilms and Microbiomes, 5(1), 34-. doi:10.1038/s41522-019-0108-3 2055-5008 https://hdl.handle.net/10356/142631 10.1038/s41522-019-0108-3 31728201 2-s2.0-85074878164 1 5 en npj Biofilms and Microbiomes © 2019 The Author(s). Published in partnership with Nanyang Technological University. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. application/pdf |
| spellingShingle | Science::Biological sciences Antibiotic Biofilm Penesyan, Anahit Nagy, Stephanie S. Kjelleberg, Staffan Gillings, Michael R. Paulsen, Ian T. Rapid microevolution of biofilm cells in response to antibiotics |
| title | Rapid microevolution of biofilm cells in response to antibiotics |
| title_full | Rapid microevolution of biofilm cells in response to antibiotics |
| title_fullStr | Rapid microevolution of biofilm cells in response to antibiotics |
| title_full_unstemmed | Rapid microevolution of biofilm cells in response to antibiotics |
| title_short | Rapid microevolution of biofilm cells in response to antibiotics |
| title_sort | rapid microevolution of biofilm cells in response to antibiotics |
| topic | Science::Biological sciences Antibiotic Biofilm |
| url | https://hdl.handle.net/10356/142631 |
| work_keys_str_mv | AT penesyananahit rapidmicroevolutionofbiofilmcellsinresponsetoantibiotics AT nagystephanies rapidmicroevolutionofbiofilmcellsinresponsetoantibiotics AT kjellebergstaffan rapidmicroevolutionofbiofilmcellsinresponsetoantibiotics AT gillingsmichaelr rapidmicroevolutionofbiofilmcellsinresponsetoantibiotics AT paulseniant rapidmicroevolutionofbiofilmcellsinresponsetoantibiotics |