Stapling a G-quadruplex specific peptide
G-quadruplex (G4) is a non-canonical four-stranded nucleic acid structure and the RHAU helicase has been identified to have high specificity for recognition of parallel-stranded G4s. We have designed and synthesized two stapled peptide analogues of the G4-specfic motif of RHAU, which preserve the G4...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/10356/143224 |
| _version_ | 1826127299189145600 |
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| author | Yaneva, Militsa Yavorova Cheong, Vee Vee Cheng, Jun Kee Lim, Kah Wai Phan, Anh Tuân |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Yaneva, Militsa Yavorova Cheong, Vee Vee Cheng, Jun Kee Lim, Kah Wai Phan, Anh Tuân |
| author_sort | Yaneva, Militsa Yavorova |
| collection | NTU |
| description | G-quadruplex (G4) is a non-canonical four-stranded nucleic acid structure and the RHAU helicase has been identified to have high specificity for recognition of parallel-stranded G4s. We have designed and synthesized two stapled peptide analogues of the G4-specfic motif of RHAU, which preserve the G4 binding ability. Characterization of these peptides identified the stapled variants to exhibit higher helical formation propensity in aqueous buffer in comparison to the native RHAU sequence. Moreover, the stapled peptides exhibit superior enzymatic stability towards α-chymotrypsin. Our stapled RHAU peptides can serve as a new tool for targeting G4 nucleic acid structures. |
| first_indexed | 2024-10-01T07:06:38Z |
| format | Journal Article |
| id | ntu-10356/143224 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:06:38Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1432242023-02-28T19:47:12Z Stapling a G-quadruplex specific peptide Yaneva, Militsa Yavorova Cheong, Vee Vee Cheng, Jun Kee Lim, Kah Wai Phan, Anh Tuân School of Physical and Mathematical Sciences NTU Institute of Structural Biology Science::Biological sciences Science::Chemistry G-quadruplex G-quadruplex-binding Protein G-quadruplex (G4) is a non-canonical four-stranded nucleic acid structure and the RHAU helicase has been identified to have high specificity for recognition of parallel-stranded G4s. We have designed and synthesized two stapled peptide analogues of the G4-specfic motif of RHAU, which preserve the G4 binding ability. Characterization of these peptides identified the stapled variants to exhibit higher helical formation propensity in aqueous buffer in comparison to the native RHAU sequence. Moreover, the stapled peptides exhibit superior enzymatic stability towards α-chymotrypsin. Our stapled RHAU peptides can serve as a new tool for targeting G4 nucleic acid structures. Ministry of Education (MOE) National Research Foundation (NRF) Accepted version This work was supported by the Singapore National Research Foundation Investigatorship (NRF-NRFI2017-09) and Singapore Ministry of Education Academic Research Fund Tier 2 (MOE2018-T2-2-029). No competing financial interests have been declared. 2020-08-14T01:55:29Z 2020-08-14T01:55:29Z 2020 Journal Article Yaneva, M. Y., Cheong, V. V., Cheng, J. K., Lim, K. W., & Phan, A. T. (2020). Stapling a G-quadruplex specific peptide. Biochemical and Biophysical Research Communications. doi:10.1016/j.bbrc.2020.02.144 0006-291X https://hdl.handle.net/10356/143224 10.1016/j.bbrc.2020.02.144 32220493 2-s2.0-85082867503 en NRF-NRFI2017-09 MOE2018- T2-2-029 Biochemical and Biophysical Research Communications © 2020 Elsevier Inc. All rights reserved. This paper was published in Biochemical and Biophysical Research Communications and is made available with permission of Elsevier Inc. application/pdf |
| spellingShingle | Science::Biological sciences Science::Chemistry G-quadruplex G-quadruplex-binding Protein Yaneva, Militsa Yavorova Cheong, Vee Vee Cheng, Jun Kee Lim, Kah Wai Phan, Anh Tuân Stapling a G-quadruplex specific peptide |
| title | Stapling a G-quadruplex specific peptide |
| title_full | Stapling a G-quadruplex specific peptide |
| title_fullStr | Stapling a G-quadruplex specific peptide |
| title_full_unstemmed | Stapling a G-quadruplex specific peptide |
| title_short | Stapling a G-quadruplex specific peptide |
| title_sort | stapling a g quadruplex specific peptide |
| topic | Science::Biological sciences Science::Chemistry G-quadruplex G-quadruplex-binding Protein |
| url | https://hdl.handle.net/10356/143224 |
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