Characterization of the refractory dissolved organic matters (rDOM) in sludge alkaline fermentation liquid driven denitrification : effect of HRT on their fate and transformation
Enhanced biological denitrification for nitrogen removal using sludge alkaline fermentation liquid (SAFL) as an alternative carbon source has been widely reported in previous studies, while limited studies focused on the degradation of the organics presented in SAFL. In this study, an SAFL driven an...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | English |
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2020
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| Online Access: | https://hdl.handle.net/10356/143247 |
| _version_ | 1811693418085089280 |
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| author | Cao, Shenbin Sun, Faqian Lu, Dan Zhou, Yan |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Cao, Shenbin Sun, Faqian Lu, Dan Zhou, Yan |
| author_sort | Cao, Shenbin |
| collection | NTU |
| description | Enhanced biological denitrification for nitrogen removal using sludge alkaline fermentation liquid (SAFL) as an alternative carbon source has been widely reported in previous studies, while limited studies focused on the degradation of the organics presented in SAFL. In this study, an SAFL driven anoxic denitrification sequencing batch reactor (SBR) was established, the mechanism of organics utilization was characterized and the refractory dissolved organic matters (rDOM) was identified. Denitrification could rapidly proceed with the presence of volatile fatty acids (VFAs) initially, while the denitrification rate largely decreased after the VFAs depleted. A great deal of rDOM, which was hard to be utilized by denitrifying microorganism, was found in the effluent. A prolonged hydraulic retention time (HRT) led to the further transformation of particles and colloids to smaller colloids and soluble organics. Extended HRT promoted the degradation of soluble microbial by-product (SMP), but had minor effect on the removal of humic-like, and fulvic acid-like substances. The characterization of the effluent demonstrated the building blocks, were dominated in the rDOM (43.79%–48.78%), followed by high molecular weight protein (HMW-PN) (13.37%–17.39%), HMW polysaccharide (HMW-PS) (12.84%–15.9%), low molecular weight (LMW) neutrals (11.28%–13.65%), and hydrophobic dissolved organic carbon (HO-DOC) (8.0%–12.62%). Moreover, it was found that the building blocks were relatively easy to be degraded with the extension of HRTs, followed by LMW-PS, LMW-PN, LMW neutrals, HMW-PN, and HMW-PS. However, further extended HRT >24 h could not improve the removal of building blocks, LMW-PS and LMW neutrals. This study, for the first time, provided insights into the transformation of organic matters produced by SAFL in a denitrification system and acted as a guide for the subsequent advanced treatment. |
| first_indexed | 2024-10-01T06:51:22Z |
| format | Journal Article |
| id | ntu-10356/143247 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:51:22Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1432472021-02-15T05:24:59Z Characterization of the refractory dissolved organic matters (rDOM) in sludge alkaline fermentation liquid driven denitrification : effect of HRT on their fate and transformation Cao, Shenbin Sun, Faqian Lu, Dan Zhou, Yan School of Civil and Environmental Engineering Advanced Environmental Biotechnology Centre Nanyang Environment and Water Research Institute Engineering::Civil engineering Denitrification Sludge Alkaline Fermentation Liquid (SAFL) Enhanced biological denitrification for nitrogen removal using sludge alkaline fermentation liquid (SAFL) as an alternative carbon source has been widely reported in previous studies, while limited studies focused on the degradation of the organics presented in SAFL. In this study, an SAFL driven anoxic denitrification sequencing batch reactor (SBR) was established, the mechanism of organics utilization was characterized and the refractory dissolved organic matters (rDOM) was identified. Denitrification could rapidly proceed with the presence of volatile fatty acids (VFAs) initially, while the denitrification rate largely decreased after the VFAs depleted. A great deal of rDOM, which was hard to be utilized by denitrifying microorganism, was found in the effluent. A prolonged hydraulic retention time (HRT) led to the further transformation of particles and colloids to smaller colloids and soluble organics. Extended HRT promoted the degradation of soluble microbial by-product (SMP), but had minor effect on the removal of humic-like, and fulvic acid-like substances. The characterization of the effluent demonstrated the building blocks, were dominated in the rDOM (43.79%–48.78%), followed by high molecular weight protein (HMW-PN) (13.37%–17.39%), HMW polysaccharide (HMW-PS) (12.84%–15.9%), low molecular weight (LMW) neutrals (11.28%–13.65%), and hydrophobic dissolved organic carbon (HO-DOC) (8.0%–12.62%). Moreover, it was found that the building blocks were relatively easy to be degraded with the extension of HRTs, followed by LMW-PS, LMW-PN, LMW neutrals, HMW-PN, and HMW-PS. However, further extended HRT >24 h could not improve the removal of building blocks, LMW-PS and LMW neutrals. This study, for the first time, provided insights into the transformation of organic matters produced by SAFL in a denitrification system and acted as a guide for the subsequent advanced treatment. Nanyang Technological University Accepted version The authors would like to thank the support of Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute (NEWRI), Nanyang Technological University. The authors would also like to thank Dr. Yuan Han from NEWRI for her help in the analysis of LC-OCD-OND results. 2020-08-17T01:13:32Z 2020-08-17T01:13:32Z 2019 Journal Article Cao, S., Sun, F., Lu, D., & Zhou, Y. (2019). Characterization of the refractory dissolved organic matters (rDOM) in sludge alkaline fermentation liquid driven denitrification : effect of HRT on their fate and transformation. Water Research, 159, 135-144. doi:10.1016/j.watres.2019.04.063 0043-1354 https://hdl.handle.net/10356/143247 10.1016/j.watres.2019.04.063 31085388 2-s2.0-85065411977 159 135 144 en Water Research © 2019 Elsevier Ltd. All rights reserved. This paper was published in Water Research and is made available with permission of Elsevier Ltd. application/pdf |
| spellingShingle | Engineering::Civil engineering Denitrification Sludge Alkaline Fermentation Liquid (SAFL) Cao, Shenbin Sun, Faqian Lu, Dan Zhou, Yan Characterization of the refractory dissolved organic matters (rDOM) in sludge alkaline fermentation liquid driven denitrification : effect of HRT on their fate and transformation |
| title | Characterization of the refractory dissolved organic matters (rDOM) in sludge alkaline fermentation liquid driven denitrification : effect of HRT on their fate and transformation |
| title_full | Characterization of the refractory dissolved organic matters (rDOM) in sludge alkaline fermentation liquid driven denitrification : effect of HRT on their fate and transformation |
| title_fullStr | Characterization of the refractory dissolved organic matters (rDOM) in sludge alkaline fermentation liquid driven denitrification : effect of HRT on their fate and transformation |
| title_full_unstemmed | Characterization of the refractory dissolved organic matters (rDOM) in sludge alkaline fermentation liquid driven denitrification : effect of HRT on their fate and transformation |
| title_short | Characterization of the refractory dissolved organic matters (rDOM) in sludge alkaline fermentation liquid driven denitrification : effect of HRT on their fate and transformation |
| title_sort | characterization of the refractory dissolved organic matters rdom in sludge alkaline fermentation liquid driven denitrification effect of hrt on their fate and transformation |
| topic | Engineering::Civil engineering Denitrification Sludge Alkaline Fermentation Liquid (SAFL) |
| url | https://hdl.handle.net/10356/143247 |
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