Developing an orally active insulin from Astragalus membranaceus
With global diabetes rates reaching epidemic levels, reliance on antidiabetic treatment will only increase. Current antidiabetic treatments lack oral delivery methods due to the peptidic nature of insulin. Digestive acids and enzymes degrade insulin before it can be absorbed into the bloodstream. Hu...
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| Format: | Final Year Project (FYP) |
| Language: | English |
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Nanyang Technological University
2020
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| Online Access: | https://hdl.handle.net/10356/143318 |
| _version_ | 1826128930856239104 |
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| author | Soo, Dylan |
| author2 | James P Tam |
| author_facet | James P Tam Soo, Dylan |
| author_sort | Soo, Dylan |
| collection | NTU |
| description | With global diabetes rates reaching epidemic levels, reliance on antidiabetic treatment will only increase. Current antidiabetic treatments lack oral delivery methods due to the peptidic nature of insulin. Digestive acids and enzymes degrade insulin before it can be absorbed into the bloodstream. Huang qi (A. Membranaceus) is listed as one of 50 fundamental herbs used in traditional Chinese medicine (TCM) and has been used in diabetic treatment. The Huang qi-derived α-astratide (aM1) is a 37 amino acid long hyper-disulfided PA1b-like peptide with three interlocking disulfide linkages granting stability against thermal, acidic and enzymatic degradation. However, very little is known about the recently discovered aM1. Here we report on the functional characterization of aM1. Toxicity studies showed that aM1 is non-toxic to cells. Cell-based glucose uptake assays showed that aM1 is an adaptogen, able to maintain cellular glucose homeostasis through increased glucose uptake in mouse muscle cell-lines. Discovery of aM1 might open a new window into developing safe orally active insulin. |
| first_indexed | 2024-10-01T07:32:36Z |
| format | Final Year Project (FYP) |
| id | ntu-10356/143318 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:32:36Z |
| publishDate | 2020 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1433182023-02-28T18:07:46Z Developing an orally active insulin from Astragalus membranaceus Soo, Dylan James P Tam School of Biological Sciences Bamaprasad Dutta JPTam@ntu.edu.sg Science::Biological sciences With global diabetes rates reaching epidemic levels, reliance on antidiabetic treatment will only increase. Current antidiabetic treatments lack oral delivery methods due to the peptidic nature of insulin. Digestive acids and enzymes degrade insulin before it can be absorbed into the bloodstream. Huang qi (A. Membranaceus) is listed as one of 50 fundamental herbs used in traditional Chinese medicine (TCM) and has been used in diabetic treatment. The Huang qi-derived α-astratide (aM1) is a 37 amino acid long hyper-disulfided PA1b-like peptide with three interlocking disulfide linkages granting stability against thermal, acidic and enzymatic degradation. However, very little is known about the recently discovered aM1. Here we report on the functional characterization of aM1. Toxicity studies showed that aM1 is non-toxic to cells. Cell-based glucose uptake assays showed that aM1 is an adaptogen, able to maintain cellular glucose homeostasis through increased glucose uptake in mouse muscle cell-lines. Discovery of aM1 might open a new window into developing safe orally active insulin. Bachelor of Science in Biomedical Sciences and BioBusiness 2020-08-24T01:21:51Z 2020-08-24T01:21:51Z 2020 Final Year Project (FYP) https://hdl.handle.net/10356/143318 en application/pdf Nanyang Technological University |
| spellingShingle | Science::Biological sciences Soo, Dylan Developing an orally active insulin from Astragalus membranaceus |
| title | Developing an orally active insulin from Astragalus membranaceus |
| title_full | Developing an orally active insulin from Astragalus membranaceus |
| title_fullStr | Developing an orally active insulin from Astragalus membranaceus |
| title_full_unstemmed | Developing an orally active insulin from Astragalus membranaceus |
| title_short | Developing an orally active insulin from Astragalus membranaceus |
| title_sort | developing an orally active insulin from astragalus membranaceus |
| topic | Science::Biological sciences |
| url | https://hdl.handle.net/10356/143318 |
| work_keys_str_mv | AT soodylan developinganorallyactiveinsulinfromastragalusmembranaceus |