Bessel-Gauss beam light sheet assisted fluorescence imaging of Trabecular meshwork in the Iridocorneal region using long working distance objectives
Glaucoma is one of the leading cause of blindness characterized by increased intra ocular pressure (IOP), visual field defects and irreversible loss of vision. Remedial intervention of glaucoma primarily aims at the reduction of IOP and subsequent examination concerning the related anomalies in the...
| المؤلفون الرئيسيون: | , , , , , |
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| مؤلفون آخرون: | |
| التنسيق: | Conference Paper |
| اللغة: | English |
| منشور في: |
2020
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://hdl.handle.net/10356/143469 |
| _version_ | 1826179033691324416 |
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| author | Sandeep, C. S. Suchand Sreelatha, Sarangapani Baskaran, Mani Hong, Jeesmond Xun Jie Aung, Tin Murukeshan, Vadakke Matham |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Sandeep, C. S. Suchand Sreelatha, Sarangapani Baskaran, Mani Hong, Jeesmond Xun Jie Aung, Tin Murukeshan, Vadakke Matham |
| author_sort | Sandeep, C. S. Suchand |
| collection | NTU |
| description | Glaucoma is one of the leading cause of blindness characterized by increased intra ocular pressure (IOP), visual field defects and irreversible loss of vision. Remedial intervention of glaucoma primarily aims at the reduction of IOP and subsequent examination concerning the related anomalies in the aqueous outflow system (AOS) especially with newer angle procedures. Thus, high resolution imaging of the iridocorneal angle (ICA) region comprising trabecular meshwork (TM) is extremely valuable to clinicians and vision analysts in comprehending the disease state for the efficacious analysis and treatment of glaucoma. Imaging of the AOS inside the eye using the digitally scanned Bessel-Gauss beam light sheet microscopy has been used in this study to obtain high resolution optical sections with minimal phototoxicity and photobleaching. This paper investigates the effect of long working distance objectives in obtaining high resolution TM images while offering non-contact and non-invasive approach in imaging. A series of experiments were conducted to optimize various imaging parameters using porcine eyes as test samples. Investigations carried out by illuminating both the anterior segment region and limbal region resulted in promising results. A delineated network of collagen fibers in a meshwork fashion can be clearly seen in the obtained images of the TM. The optical sectioning capability of this technique is demonstrated and the structural features match well with previous literature reports. |
| first_indexed | 2024-10-01T03:31:13Z |
| format | Conference Paper |
| id | ntu-10356/143469 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:31:13Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1434692023-03-04T17:07:15Z Bessel-Gauss beam light sheet assisted fluorescence imaging of Trabecular meshwork in the Iridocorneal region using long working distance objectives Sandeep, C. S. Suchand Sreelatha, Sarangapani Baskaran, Mani Hong, Jeesmond Xun Jie Aung, Tin Murukeshan, Vadakke Matham School of Mechanical and Aerospace Engineering 2019 9th International Conference on Bioscience, Biochemistry and Bioinformatics (ICBBB 2019) Centre for Optical & Laser Engineering Singapore Centre for 3D Printing Engineering::Mechanical engineering Bio Optics Bessel–Gauss Beam Glaucoma is one of the leading cause of blindness characterized by increased intra ocular pressure (IOP), visual field defects and irreversible loss of vision. Remedial intervention of glaucoma primarily aims at the reduction of IOP and subsequent examination concerning the related anomalies in the aqueous outflow system (AOS) especially with newer angle procedures. Thus, high resolution imaging of the iridocorneal angle (ICA) region comprising trabecular meshwork (TM) is extremely valuable to clinicians and vision analysts in comprehending the disease state for the efficacious analysis and treatment of glaucoma. Imaging of the AOS inside the eye using the digitally scanned Bessel-Gauss beam light sheet microscopy has been used in this study to obtain high resolution optical sections with minimal phototoxicity and photobleaching. This paper investigates the effect of long working distance objectives in obtaining high resolution TM images while offering non-contact and non-invasive approach in imaging. A series of experiments were conducted to optimize various imaging parameters using porcine eyes as test samples. Investigations carried out by illuminating both the anterior segment region and limbal region resulted in promising results. A delineated network of collagen fibers in a meshwork fashion can be clearly seen in the obtained images of the TM. The optical sectioning capability of this technique is demonstrated and the structural features match well with previous literature reports. Agency for Science, Technology and Research (A*STAR) Accepted version The authors acknowledge the financial support received through A*STAR-MIG project (BMRC1619077002) and the facilities and research manpower provided through COLE-EDB funding. 2020-09-03T05:11:07Z 2020-09-03T05:11:07Z 2019 Conference Paper Sandeep, C. S. S., Sreelatha, S., Baskaran, M., Hong, J. X. J., Aung, T., & Murukeshan, V. M. (2019). Bessel-Gauss beam light sheet assisted fluorescence imaging of Trabecular meshwork in the Iridocorneal region using long working distance objectives. Proceedings of the 2019 9th International Conference on Bioscience, Biochemistry and Bioinformatics (ICBBB 2019), 8-11. doi:10.1145/3314367.3314380 978-1-4503-6654-0 https://hdl.handle.net/10356/143469 10.1145/3314367.3314380 2-s2.0-85065257019 8 11 en © 2019 Association for Computing Machinery. All rights reserved. This paper was published in Proceedings of the 2019 9th International Conference on Bioscience, Biochemistry and Bioinformatics (ICBBB 2019) and is made available with permission of Association for Computing Machinery. application/pdf |
| spellingShingle | Engineering::Mechanical engineering Bio Optics Bessel–Gauss Beam Sandeep, C. S. Suchand Sreelatha, Sarangapani Baskaran, Mani Hong, Jeesmond Xun Jie Aung, Tin Murukeshan, Vadakke Matham Bessel-Gauss beam light sheet assisted fluorescence imaging of Trabecular meshwork in the Iridocorneal region using long working distance objectives |
| title | Bessel-Gauss beam light sheet assisted fluorescence imaging of Trabecular meshwork in the Iridocorneal region using long working distance objectives |
| title_full | Bessel-Gauss beam light sheet assisted fluorescence imaging of Trabecular meshwork in the Iridocorneal region using long working distance objectives |
| title_fullStr | Bessel-Gauss beam light sheet assisted fluorescence imaging of Trabecular meshwork in the Iridocorneal region using long working distance objectives |
| title_full_unstemmed | Bessel-Gauss beam light sheet assisted fluorescence imaging of Trabecular meshwork in the Iridocorneal region using long working distance objectives |
| title_short | Bessel-Gauss beam light sheet assisted fluorescence imaging of Trabecular meshwork in the Iridocorneal region using long working distance objectives |
| title_sort | bessel gauss beam light sheet assisted fluorescence imaging of trabecular meshwork in the iridocorneal region using long working distance objectives |
| topic | Engineering::Mechanical engineering Bio Optics Bessel–Gauss Beam |
| url | https://hdl.handle.net/10356/143469 |
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