Multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions
Cells sense biochemical as well as mechanical signals from their microenvironment by engaging multiple receptors. In many cases, multiple receptors operate in concert, and it can be misleading to attempt to isolate a single ligand–receptor interaction. Supported lipid membranes are employed to recon...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/143720 |
| _version_ | 1826125654343548928 |
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| author | Biswas, Kabir H. Chen, Zhongwen Dubey, Alok Kumar Oh, Dongmyung Groves, Jay T. |
| author2 | School of Materials Science and Engineering |
| author_facet | School of Materials Science and Engineering Biswas, Kabir H. Chen, Zhongwen Dubey, Alok Kumar Oh, Dongmyung Groves, Jay T. |
| author_sort | Biswas, Kabir H. |
| collection | NTU |
| description | Cells sense biochemical as well as mechanical signals from their microenvironment by engaging multiple receptors. In many cases, multiple receptors operate in concert, and it can be misleading to attempt to isolate a single ligand–receptor interaction. Supported lipid membranes are employed to reconstitute a number of cell receptor systems. Efforts are also made to fabricate membrane microarrays presenting multiple ligands in a spatially segregated manner. However, such membrane multiplexing methods are generally limited by complex instrumentation and scalability. Here, a straightforward method is presented to produce centimeter‐scale membrane microarrays displaying multiple, spatially segregated membrane‐anchored protein ligands suitable for live single‐cell studies. The method is based on stochastic membrane fusion and subsequent diffusion‐mediated mixing of their lipid content. The result is the delivery of membrane contents into spatially segregated membrane corrals. Utilizing the technology developed here, this work probes the recruitment of an adaptor protein, Shc1, to epidermal growth factor receptor (EGFR) and EphA2 receptors and shows that activation of EGFR results in a decrease in the recruitment of protein to activate EphA2 same cell. |
| first_indexed | 2024-10-01T06:39:52Z |
| format | Journal Article |
| id | ntu-10356/143720 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:39:52Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1437202020-09-18T08:09:41Z Multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions Biswas, Kabir H. Chen, Zhongwen Dubey, Alok Kumar Oh, Dongmyung Groves, Jay T. School of Materials Science and Engineering Engineering::Materials Cell Signaling Lipid Exchange Cells sense biochemical as well as mechanical signals from their microenvironment by engaging multiple receptors. In many cases, multiple receptors operate in concert, and it can be misleading to attempt to isolate a single ligand–receptor interaction. Supported lipid membranes are employed to reconstitute a number of cell receptor systems. Efforts are also made to fabricate membrane microarrays presenting multiple ligands in a spatially segregated manner. However, such membrane multiplexing methods are generally limited by complex instrumentation and scalability. Here, a straightforward method is presented to produce centimeter‐scale membrane microarrays displaying multiple, spatially segregated membrane‐anchored protein ligands suitable for live single‐cell studies. The method is based on stochastic membrane fusion and subsequent diffusion‐mediated mixing of their lipid content. The result is the delivery of membrane contents into spatially segregated membrane corrals. Utilizing the technology developed here, this work probes the recruitment of an adaptor protein, Shc1, to epidermal growth factor receptor (EGFR) and EphA2 receptors and shows that activation of EGFR results in a decrease in the recruitment of protein to activate EphA2 same cell. National Research Foundation (NRF) 2020-09-18T08:09:41Z 2020-09-18T08:09:41Z 2018 Journal Article Biswas, K. H., Chen, Z., Dubey, A. K., Oh, D., & Groves, J. T. (2018). Multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions. Advanced Biosystems, 2(4), 1800015-. doi:10.1002/adbi.201800015 2366-7478 https://hdl.handle.net/10356/143720 10.1002/adbi.201800015 4 2 1800015 en Advanced Biosystems © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved. |
| spellingShingle | Engineering::Materials Cell Signaling Lipid Exchange Biswas, Kabir H. Chen, Zhongwen Dubey, Alok Kumar Oh, Dongmyung Groves, Jay T. Multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions |
| title | Multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions |
| title_full | Multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions |
| title_fullStr | Multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions |
| title_full_unstemmed | Multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions |
| title_short | Multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions |
| title_sort | multicomponent supported membrane microarray for monitoring spatially resolved cellular signaling reactions |
| topic | Engineering::Materials Cell Signaling Lipid Exchange |
| url | https://hdl.handle.net/10356/143720 |
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