A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state
The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols re...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/143817 |
| _version_ | 1811692654820327424 |
|---|---|
| author | Szczerbinska, Iwona Gonzales, Kevin Andrew Uy Cukuroglu, Engin Muhammad Nadzim Bin Ramli Lee, Bertha Pei Ge Tan, Cheng Peow Wong, Cheng Kit Rancati, Giulia Irene Liang, Hongqing Göke, Jonathan Ng, Huck-Hui Chan, Yun-Shen |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Szczerbinska, Iwona Gonzales, Kevin Andrew Uy Cukuroglu, Engin Muhammad Nadzim Bin Ramli Lee, Bertha Pei Ge Tan, Cheng Peow Wong, Cheng Kit Rancati, Giulia Irene Liang, Hongqing Göke, Jonathan Ng, Huck-Hui Chan, Yun-Shen |
| author_sort | Szczerbinska, Iwona |
| collection | NTU |
| description | The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols relying on extraneous undefined factors such as feeders. Here we performed a small-molecule screen to identify compounds that facilitate chemically defined establishment and maintenance of human feeder-independent naive embryonic (FINE) stem cells. The expression profile in genic and repetitive elements of FINE cells resembles the 8-cell-to-morula stage in vivo, and only differs from feeder-dependent naive cells in genes involved in cell-cell/cell-matrix interactions. FINE cells offer several technical advantages, such as increased amenability to transfection and a longer period of genomic stability, compared with feeder-dependent cells. Thus, FINE cells will serve as an accessible and useful system for scientific and translational applications of naïve pluripotent stem cells. |
| first_indexed | 2024-10-01T06:39:14Z |
| format | Journal Article |
| id | ntu-10356/143817 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:39:14Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1438172023-02-28T17:09:25Z A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state Szczerbinska, Iwona Gonzales, Kevin Andrew Uy Cukuroglu, Engin Muhammad Nadzim Bin Ramli Lee, Bertha Pei Ge Tan, Cheng Peow Wong, Cheng Kit Rancati, Giulia Irene Liang, Hongqing Göke, Jonathan Ng, Huck-Hui Chan, Yun-Shen School of Biological Sciences Science::Biological sciences Feeder-free System Naive Pluripotent State The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols relying on extraneous undefined factors such as feeders. Here we performed a small-molecule screen to identify compounds that facilitate chemically defined establishment and maintenance of human feeder-independent naive embryonic (FINE) stem cells. The expression profile in genic and repetitive elements of FINE cells resembles the 8-cell-to-morula stage in vivo, and only differs from feeder-dependent naive cells in genes involved in cell-cell/cell-matrix interactions. FINE cells offer several technical advantages, such as increased amenability to transfection and a longer period of genomic stability, compared with feeder-dependent cells. Thus, FINE cells will serve as an accessible and useful system for scientific and translational applications of naïve pluripotent stem cells. Agency for Science, Technology and Research (A*STAR) Ministry of Health (MOH) National Medical Research Council (NMRC) National Research Foundation (NRF) Published version 2020-09-25T01:53:01Z 2020-09-25T01:53:01Z 2019 Journal Article Szczerbinska, I., Gonzales, K. A. U., Cukuroglu, E., Muhammad Nadzim Bin Ramli, Lee, B. P. G., Tan, C. P., ... Chan, Y.-S. (2019). A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state. Stem Cell Reports, 13(4), 612-626. doi:10.1016/j.stemcr.2019.08.005 2213-6711 https://hdl.handle.net/10356/143817 10.1016/j.stemcr.2019.08.005 31522974 4 13 612 626 en Stem Cell Reports © 2019 The Author(s).This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). application/pdf |
| spellingShingle | Science::Biological sciences Feeder-free System Naive Pluripotent State Szczerbinska, Iwona Gonzales, Kevin Andrew Uy Cukuroglu, Engin Muhammad Nadzim Bin Ramli Lee, Bertha Pei Ge Tan, Cheng Peow Wong, Cheng Kit Rancati, Giulia Irene Liang, Hongqing Göke, Jonathan Ng, Huck-Hui Chan, Yun-Shen A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state |
| title | A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state |
| title_full | A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state |
| title_fullStr | A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state |
| title_full_unstemmed | A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state |
| title_short | A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state |
| title_sort | chemically defined feeder free system for the establishment and maintenance of the human naive pluripotent state |
| topic | Science::Biological sciences Feeder-free System Naive Pluripotent State |
| url | https://hdl.handle.net/10356/143817 |
| work_keys_str_mv | AT szczerbinskaiwona achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT gonzaleskevinandrewuy achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT cukurogluengin achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT muhammadnadzimbinramli achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT leeberthapeige achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT tanchengpeow achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT wongchengkit achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT rancatigiuliairene achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT lianghongqing achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT gokejonathan achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT nghuckhui achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT chanyunshen achemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT szczerbinskaiwona chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT gonzaleskevinandrewuy chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT cukurogluengin chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT muhammadnadzimbinramli chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT leeberthapeige chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT tanchengpeow chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT wongchengkit chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT rancatigiuliairene chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT lianghongqing chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT gokejonathan chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT nghuckhui chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate AT chanyunshen chemicallydefinedfeederfreesystemfortheestablishmentandmaintenanceofthehumannaivepluripotentstate |