Extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events
A larger number of human diseases are related to dysregulation or loss of cellular functions. Effective restoration of the missing or defective cellular functions is highly desirable for fundamental research and therapeutic applications. Inspired by the fantastic feature of cell-derived extracellula...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/143960 |
| _version_ | 1826116101605425152 |
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| author | Lyu, Linna Hu, Ming Fu, Afu Xing, Bengang |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Lyu, Linna Hu, Ming Fu, Afu Xing, Bengang |
| author_sort | Lyu, Linna |
| collection | NTU |
| description | A larger number of human diseases are related to dysregulation or loss of cellular functions. Effective restoration of the missing or defective cellular functions is highly desirable for fundamental research and therapeutic applications. Inspired by the fantastic feature of cell-derived extracellular vesicles (EVs) that can transport various bioactive molecules between cells, herein, we developed a simple and efficient strategy based on EVs for transferring ion channels to recipient cells, thereby conferring specific biological function to the target cells and regulating the biological events. The constructed channel rhodopsin 2 (ChR2)-loaded EV (EV-ChR2) system can mediate the anchor of light-responsive ion channel ChR2 on the plasma membrane of recipient cells through membrane fusion. Upon blue light irradiation, the ion channel ChR2 was activated and opened, thus permitting the rapid flux of cation ions (e.g., calcium ion) across the plasma membrane of recipient cells. Moreover, the increased Ca2+ in the cytosol could effectively activate Ca2+-dependent transcription factors, further triggering the calcium signaling pathway. This strategy can be extended to modulate other cellular processes and provides a novel insight on the manipulation of biological events. |
| first_indexed | 2024-10-01T04:05:53Z |
| format | Journal Article |
| id | ntu-10356/143960 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:05:53Z |
| publishDate | 2020 |
| record_format | dspace |
| spelling | ntu-10356/1439602020-10-05T04:07:01Z Extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events Lyu, Linna Hu, Ming Fu, Afu Xing, Bengang School of Chemical and Biomedical Engineering School of Physical and Mathematical Sciences Science::Chemistry::Biochemistry Peptides and Proteins Plasma Membrane A larger number of human diseases are related to dysregulation or loss of cellular functions. Effective restoration of the missing or defective cellular functions is highly desirable for fundamental research and therapeutic applications. Inspired by the fantastic feature of cell-derived extracellular vesicles (EVs) that can transport various bioactive molecules between cells, herein, we developed a simple and efficient strategy based on EVs for transferring ion channels to recipient cells, thereby conferring specific biological function to the target cells and regulating the biological events. The constructed channel rhodopsin 2 (ChR2)-loaded EV (EV-ChR2) system can mediate the anchor of light-responsive ion channel ChR2 on the plasma membrane of recipient cells through membrane fusion. Upon blue light irradiation, the ion channel ChR2 was activated and opened, thus permitting the rapid flux of cation ions (e.g., calcium ion) across the plasma membrane of recipient cells. Moreover, the increased Ca2+ in the cytosol could effectively activate Ca2+-dependent transcription factors, further triggering the calcium signaling pathway. This strategy can be extended to modulate other cellular processes and provides a novel insight on the manipulation of biological events. 2020-10-05T04:07:01Z 2020-10-05T04:07:01Z 2018 Journal Article Lyu, L., Hu, M., Fu, A., & Xing, B. (2018). Extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events. Bioconjugate Chemistry, 29(8), 2715-2722. doi:10.1021/acs.bioconjchem.8b00377 1520-4812 https://hdl.handle.net/10356/143960 10.1021/acs.bioconjchem.8b00377 29952546 8 29 2715 2722 en Bioconjugate chemistry © 2018 American Chemical Society. All rights reserved. |
| spellingShingle | Science::Chemistry::Biochemistry Peptides and Proteins Plasma Membrane Lyu, Linna Hu, Ming Fu, Afu Xing, Bengang Extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events |
| title | Extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events |
| title_full | Extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events |
| title_fullStr | Extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events |
| title_full_unstemmed | Extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events |
| title_short | Extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events |
| title_sort | extracellular vesicle directed exogenous ion channel transport for precise manipulation of biological events |
| topic | Science::Chemistry::Biochemistry Peptides and Proteins Plasma Membrane |
| url | https://hdl.handle.net/10356/143960 |
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