Investigation of the microbial community structure in enhanced anaerobic sludge digestion
Sludge is an inevitable by-product in the current wastewater treatment process utilizing activated sludge technology, and its treatment and disposal could account up to 50% of WWTP’s operating costs. Optimization of the anaerobic sludge digestion to enhance volatile solids (VS) reduction and energy...
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| Format: | Thesis-Doctor of Philosophy |
| Language: | English |
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Nanyang Technological University
2020
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| Online Access: | https://hdl.handle.net/10356/144046 |
| _version_ | 1826113075796770816 |
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| author | Maspolim, Yogananda |
| author2 | Ng Wun Jern |
| author_facet | Ng Wun Jern Maspolim, Yogananda |
| author_sort | Maspolim, Yogananda |
| collection | NTU |
| description | Sludge is an inevitable by-product in the current wastewater treatment process utilizing activated sludge technology, and its treatment and disposal could account up to 50% of WWTP’s operating costs. Optimization of the anaerobic sludge digestion to enhance volatile solids (VS) reduction and energy recovery (via methane production) is critical in the face of global challenges today. Process improvement employing biological methods (two-stage system) is preferred due to its high digestion efficiency and relatively lower operating cost and energy requirement compared to other methods. However, the underlying microbial diversity, its interactions, functions and responses to operational or environmental conditions are not clearly understood. In this study, sludge digestion enhancement efforts are complemented with microbiological molecular tools including denaturing gradient gel electrophoresis (DGGE), quantitative polymerase chain reaction (qPCR) and high throughput 454 pyrosequencing to address this research gap. |
| first_indexed | 2024-10-01T03:17:34Z |
| format | Thesis-Doctor of Philosophy |
| id | ntu-10356/144046 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:17:34Z |
| publishDate | 2020 |
| publisher | Nanyang Technological University |
| record_format | dspace |
| spelling | ntu-10356/1440462021-01-07T00:56:27Z Investigation of the microbial community structure in enhanced anaerobic sludge digestion Maspolim, Yogananda Ng Wun Jern School of Civil and Environmental Engineering WJNg@ntu.edu.sg Engineering::Environmental engineering Sludge is an inevitable by-product in the current wastewater treatment process utilizing activated sludge technology, and its treatment and disposal could account up to 50% of WWTP’s operating costs. Optimization of the anaerobic sludge digestion to enhance volatile solids (VS) reduction and energy recovery (via methane production) is critical in the face of global challenges today. Process improvement employing biological methods (two-stage system) is preferred due to its high digestion efficiency and relatively lower operating cost and energy requirement compared to other methods. However, the underlying microbial diversity, its interactions, functions and responses to operational or environmental conditions are not clearly understood. In this study, sludge digestion enhancement efforts are complemented with microbiological molecular tools including denaturing gradient gel electrophoresis (DGGE), quantitative polymerase chain reaction (qPCR) and high throughput 454 pyrosequencing to address this research gap. Doctor of Philosophy 2020-10-09T07:19:10Z 2020-10-09T07:19:10Z 2016 Thesis-Doctor of Philosophy Maspolim, Y. (2016). Investigation of the microbial community structure in enhanced anaerobic sludge digestion. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/144046 10.32657/10356/144046 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University |
| spellingShingle | Engineering::Environmental engineering Maspolim, Yogananda Investigation of the microbial community structure in enhanced anaerobic sludge digestion |
| title | Investigation of the microbial community structure in enhanced anaerobic sludge digestion |
| title_full | Investigation of the microbial community structure in enhanced anaerobic sludge digestion |
| title_fullStr | Investigation of the microbial community structure in enhanced anaerobic sludge digestion |
| title_full_unstemmed | Investigation of the microbial community structure in enhanced anaerobic sludge digestion |
| title_short | Investigation of the microbial community structure in enhanced anaerobic sludge digestion |
| title_sort | investigation of the microbial community structure in enhanced anaerobic sludge digestion |
| topic | Engineering::Environmental engineering |
| url | https://hdl.handle.net/10356/144046 |
| work_keys_str_mv | AT maspolimyogananda investigationofthemicrobialcommunitystructureinenhancedanaerobicsludgedigestion |